Jing Luo1, Chengfei Yang1, Xing Luo1, Yang Yang1, Jia Li1, Bo Song1, Jiang Zhao1, Longkun Li2. 1. Department of Urology, Second Affiliated Hospital, Army Military Medical University, Chongqing, People's Republic of China. 2. Department of Urology, Second Affiliated Hospital, Army Military Medical University, Chongqing, People's Republic of China. Electronic address: lilongk@hotmail.com.
Abstract
AIMS: This study aimed to investigate the therapeutic effect and molecular mechanism of chlorogenic acid (CGA) on cyclophosphamide (CYP)-induced rat interstitial cystitis (IC). MATERIALS AND METHODS: An animal model of IC was established by intraperitoneal injection of CYP in female Sprague-Dawley rats. Eighty rats were randomly assigned to four groups: negative control (NC), NC treated with CGA (NC + CGA), IC, and IC treated with CGA (IC + CGA). Bladder urination function was assessed by analyzing urodynamic parameters. The expression of apoptosis-related proteins and inflammatory biomarkers in bladder specimens was detected using western blot and immunohistochemistry analysis. KEY FINDINGS: Compared with the IC group, bladder urinary function was significantly improved in the IC + CGA group. CGA treatment reduced inflammatory damage in the bladder tissue of IC rats. Caspase3 and Bax expression was higher while Bcl-2 expression was lower in the IC group compared to the IC + CGA group. In addition, there were significant differences between the groups in the expression levels of inflammatory biomarkers in the bladder tissue. Furthermore, CGA could inhibit CYP-induced MAPK/NF-κB phosphorylation in the rat bladder tissue. SIGNIFICANCE: In a CYP-induced rat model of IC, CGA could reduce inflammation and apoptosis, thus partially restoring bladder function, and the MAPK/NF-κB pathway was probably involved in it.
AIMS: This study aimed to investigate the therapeutic effect and molecular mechanism of chlorogenic acid (CGA) on cyclophosphamide (CYP)-induced ratinterstitial cystitis (IC). MATERIALS AND METHODS: An animal model of IC was established by intraperitoneal injection of CYP in female Sprague-Dawley rats. Eighty rats were randomly assigned to four groups: negative control (NC), NC treated with CGA (NC + CGA), IC, and IC treated with CGA (IC + CGA). Bladder urination function was assessed by analyzing urodynamic parameters. The expression of apoptosis-related proteins and inflammatory biomarkers in bladder specimens was detected using western blot and immunohistochemistry analysis. KEY FINDINGS: Compared with the IC group, bladder urinary function was significantly improved in the IC + CGA group. CGA treatment reduced inflammatory damage in the bladder tissue of ICrats. Caspase3 and Bax expression was higher while Bcl-2 expression was lower in the IC group compared to the IC + CGA group. In addition, there were significant differences between the groups in the expression levels of inflammatory biomarkers in the bladder tissue. Furthermore, CGA could inhibit CYP-induced MAPK/NF-κB phosphorylation in the rat bladder tissue. SIGNIFICANCE: In a CYP-induced rat model of IC, CGA could reduce inflammation and apoptosis, thus partially restoring bladder function, and the MAPK/NF-κB pathway was probably involved in it.
Authors: Lucas Solyano Almeida de Oliveira; Sara Raquel de Moura Bandeira; Rodrigo Lopes Gomes Gonçalves; Benedito Pereira de Sousa Neto; Diana Carvalho de Rezende; Antonio Carlos Dos Reis-Filho; Ian Jhemes Oliveira Sousa; Flaviano Ribeiro Pinheiro-Neto; Boris Timah Acha; Gabriela do Nascimento Caldas Trindade; Lázaro Gomes do Nascimento; Damião Pergentino de Sousa; Fernanda Regina de Castro Almeida; Massimo Lucarini; Alessandra Durazzo; Daniel Dias Rufino Arcanjo; Francisco de Assis Oliveira Journal: Biology (Basel) Date: 2022-05-09
Authors: Il-Gyu Ko; Jun-Jang Jin; Lakkyong Hwang; Sang-Hoon Kim; Chang-Ju Kim; Kyu Yeoun Won; Yong Gil Na; Khae Hawn Kim; Su Jin Kim Journal: J Inflamm Res Date: 2021-02-15