Liya Li1, Xiaoxia Zuo2, Yizhi Xiao3, Di Liu4, Hui Luo5, Honglin Zhu6. 1. Department of Rheumatology and Immunology, Xiangya Hospital, Central South University, Changsha, China; The Institute of Rheumatology and Immunology, Central South University, Changsha, China. Electronic address: liliyacsu@sina.com. 2. Department of Rheumatology and Immunology, Xiangya Hospital, Central South University, Changsha, China; The Institute of Rheumatology and Immunology, Central South University, Changsha, China. Electronic address: susanzuo@csu.edu.cn. 3. Department of Rheumatology and Immunology, Xiangya Hospital, Central South University, Changsha, China; The Institute of Rheumatology and Immunology, Central South University, Changsha, China. Electronic address: yizhixiao0501@outlook.com. 4. Department of Rheumatology and Immunology, Xiangya Hospital, Central South University, Changsha, China; The Institute of Rheumatology and Immunology, Central South University, Changsha, China. Electronic address: liudixy@csu.edu.cn. 5. Department of Rheumatology and Immunology, Xiangya Hospital, Central South University, Changsha, China; The Institute of Rheumatology and Immunology, Central South University, Changsha, China. Electronic address: luohui@csu.edu.cn. 6. Department of Rheumatology and Immunology, Xiangya Hospital, Central South University, Changsha, China; The Institute of Rheumatology and Immunology, Central South University, Changsha, China. Electronic address: honglinzhu@csu.edu.cn.
Abstract
OBJECTIVES: Systemic sclerosis (SSc) is an autoimmune disease characterized by vasculopathy, inflammation, and extensive fibrosis in multiple organs. Exosomes (EXOs) are cell-derived vesicles contained various DNAs, RNAs and proteins, and play important roles in various diseases. Here, we aimed to investigate the roles of SSc EXOs in angiogenesis related mechanisms. METHODS: EXOs were isolated from plasma, cultured peripheral blood mononuclear cells (PBMCs)/neutrophil supernatants, and identified by transmission electron microscopy. The expression of S100A8/A9 was measured by real-time PCR and ELISA. Proliferation, migration and scratch assays in human dermal microvascular endothelial cells (HDMECs) were used to study the EXOs influence. RESULTS: Plasma and neutrophil EXOs from SSc patients can suppress the proliferation and migration of HDMECs. High levels of S100A8/A9 were found in SSc EXOs which derived from plasma, PBMCs and neutrophils. The expression of S100A8/A9 in neutrophil EXOs was higher than that in PBMC EXOs in SSc patients. The proliferation and migration of HDMECs were possibly inhibited by S100A8/A9 of neutrophil EXOs. CONCLUSIONS: Neutrophil EXOs from SSc patients inhibits the proliferation and migration of HDMECs, S100A8/A9 might play an important role in this process.
OBJECTIVES:Systemic sclerosis (SSc) is an autoimmune disease characterized by vasculopathy, inflammation, and extensive fibrosis in multiple organs. Exosomes (EXOs) are cell-derived vesicles contained various DNAs, RNAs and proteins, and play important roles in various diseases. Here, we aimed to investigate the roles of SSc EXOs in angiogenesis related mechanisms. METHODS: EXOs were isolated from plasma, cultured peripheral blood mononuclear cells (PBMCs)/neutrophil supernatants, and identified by transmission electron microscopy. The expression of S100A8/A9 was measured by real-time PCR and ELISA. Proliferation, migration and scratch assays in human dermal microvascular endothelial cells (HDMECs) were used to study the EXOs influence. RESULTS: Plasma and neutrophil EXOs from SSc patients can suppress the proliferation and migration of HDMECs. High levels of S100A8/A9 were found in SSc EXOs which derived from plasma, PBMCs and neutrophils. The expression of S100A8/A9 in neutrophil EXOs was higher than that in PBMC EXOs in SSc patients. The proliferation and migration of HDMECs were possibly inhibited by S100A8/A9 of neutrophil EXOs. CONCLUSIONS: Neutrophil EXOs from SSc patients inhibits the proliferation and migration of HDMECs, S100A8/A9 might play an important role in this process.
Authors: Ali Hazrati; Sara Soudi; Kosar Malekpour; Mohammad Mahmoudi; Arezou Rahimi; Seyed Mahmoud Hashemi; Rajender S Varma Journal: Biomark Res Date: 2022-05-12