A MADS-box transcription factor FoRlm1 regulates aerial hyphal growth, oxidative stress, cell wall biosynthesis and virulence in Fusarium oxysporum f. sp. cubense.

The fungal pathogen Fusarium oxysporum f. sp. cubense (Foc) causes Fusarium wilt that affects banana plants. However, the detailed molecular mechanisms of Foc virulence determinants have not been elucidated. In this study, we identified the MADS-box transcription factor FoRlm1 that is conserved among mitogen-activated protein kinases. Our data revealed that FoRlm1 is essential for aerial hyphal growth and virulence. Transcriptional analysis revealed that FoRlm1 deletion altered the expression of anti-oxidant enzymes, chitin synthases, fusaric acid (FA), and beauvericin biosynthesis genes. Furthermore, FoRlm1 deletion promoted tolerance to Congo red and increased sensitivity to hydrogen peroxide. Transcriptome analysis of ΔFoRlm1 mutant and wild-type strain indicated that the expression of many genes associated with fungal physiology and virulence was up- or down-regulated. Overall, these results suggested that FoRlm1 plays a critical role in the regulation of hyphal growth, anti-oxidation mechanisms, cell wall biosynthesis, transcription of mycotoxin biosynthetic genes encoding FA and beauvericin, and virulence in Foc.