| Literature DB >> 32218683 |
Jeesoo Kim1, Thi Thanh Hanh Nguyen1, Juhui Jin1, Iis Septiana1, Gyu-Min Son1, Gang-Hee Lee1, You-Jin Jung1, Dilshad Qureshi2, Il Kyoon Mok1, Kunal Pal2, Soo-Yeon Yang1, Seong-Bo Kim3, Doman Kim1.
Abstract
Streptococcus mutans plays an important role in the development of dental caries in humans by synthesizing adhesive insoluble glucans from sucrose by mutansucrase activity. To explore the anti-cariogenic characteristics of rubusoside (Ru), a natural sweetener component in Rubus suavissimus S. Lee (Rosaceae), we investigated the inhibitory effect of Ru against the activity of mutansucrase and the growth of Streptococcus mutans. Ru (50 mM) showed 97% inhibitory activity against 0.1 U/mL mutansucrase of S. mutans with 500 mM sucrose. It showed competitive inhibition with a K i value of 1.1 ± 0.2 mM and IC50 of 2.3 mM. Its inhibition activity was due to hydrophobic and hydrogen bonding interactions based on molecular docking analysis. Ru inhibited the growth of S. mutans as a bacteriostatic agent, with MIC and MBC values of 6 mM and 8 mM, respectively. In addition, Ru showed synergistic anti-bacterial activity when it was combined with curcumin. Therefore, Ru is a natural anti-cariogenic agent with anti-mutansucrase activity and antimicrobial activity against S. mutans. ELECTRONIC SUPPLEMENTARY MATERIAL ESM: The online version of this article (doi: 10.1007/s12257-018-0408-0) contains supplementary material, which is available to authorized users. © The Korean Society for Biotechnology and Bioengineering and Springer 2019.Entities:
Keywords: Anti-cariogenicity; Streptococcus mutans; mutansucrase; natural sweetener; rubusoside
Year: 2019 PMID： 32218683 PMCID： PMC7090897 DOI： 10.1007/s12257-018-0408-0
Source DB: PubMed Journal: Biotechnol Bioprocess Eng ISSN： 1226-8372 Impact factor: 2.836