Literature DB >> 32216463

Validation and identification of reference genes in Chinese hamster ovary cells for Fc-fusion protein production.

Xiaonan Ma1, Ling Zhang1, Luming Zhang1, Chenglong Wang1, Xiaorui Guo1, Yu Yang1, Lin Wang1, Xiangru Li1, Ningning Ma1.   

Abstract

IMPACT STATEMENT: In order to reveal potential genotype-phenotype relationship, RT-qPCR reactions are frequently applied which require validated and reliable reference genes. With the investigation on long-term passage and fed-batch cultivation of CHO cells producing an Fc-fusion protein, four new reference genes-Akr1a1, Gpx1, Aprt, and Rps16, were identified from 20 candidates with the aid of geNorm, NormFinder, BestKeeper, and ΔCt programs and methods. This article provided more verified options in reference gene selection in related research on CHO cells.

Entities:  

Keywords:  Chinese hamster ovary cell; RNA-seq; real-time quantitative PCR; reference gene

Mesh：

Substances：

Year:  2020        PMID： 32216463      PMCID： PMC7372735          DOI： 10.1177/1535370220914058

Source DB:  PubMed          Journal:  Exp Biol Med (Maywood)        ISSN： 1535-3699


  60 in total

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Authors:  T Suzuki; P J Higgins; D R Crawford
Journal:  Biotechniques       Date:  2000-08       Impact factor: 1.993

Review 5.  An 'omics approach towards CHO cell engineering.

Authors:  Payel Datta; Robert J Linhardt; Susan T Sharfstein
Journal:  Biotechnol Bioeng       Date:  2013-02-04       Impact factor: 4.530

6.  Validation of endogenous controls for gene expression studies in human adipocytes and preadipocytes.

Authors:  K Gorzelniak; J Janke; S Engeli; A M Sharma
Journal:  Horm Metab Res       Date:  2001-10       Impact factor: 2.936

7.  Ablation of aldehyde reductase aggravates carbon tetrachloride-induced acute hepatic injury involving oxidative stress and endoplasmic reticulum stress.

Authors:  Ryusuke Akihara; Takujiro Homma; Jaeyong Lee; Ken-Ichi Yamada; Satoshi Miyata; Junichi Fujii
Journal:  Biochem Biophys Res Commun       Date:  2016-08-05       Impact factor: 3.575

8.  Using microarray technology to select housekeeping genes in Chinese hamster ovary cells.

Authors:  Scott M Bahr; Trissa Borgschulte; Kevin J Kayser; Nan Lin
Journal:  Biotechnol Bioeng       Date:  2009-12-01       Impact factor: 4.530

9.  Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction.

Authors:  Jian Ye; George Coulouris; Irena Zaretskaya; Ioana Cutcutache; Steve Rozen; Thomas L Madden
Journal:  BMC Bioinformatics       Date:  2012-06-18       Impact factor: 3.169

10.  Selection of suitable reference genes for quantitative real-time PCR gene expression analysis in Salix matsudana under different abiotic stresses.

Authors:  Yunxing Zhang; Xiaojiao Han; Shuangshuang Chen; Liu Zheng; Xuelian He; Mingying Liu; Guirong Qiao; Yang Wang; Renying Zhuo
Journal:  Sci Rep       Date:  2017-01-25       Impact factor: 4.379
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