| Literature DB >> 32214723 |
Haixue Zheng1,2, Xiangtao Liu1,2, Youjun Shang1, Jinyan Wu1, Xingwen Bai1, Ye Jin1, Shiqi Sun1,2, Huichen Guo1, Hong Tian1, Xia Feng1, Shuanghui Yin1, Jianhong Guo1,2, Guozheng Cong1,2, Zaixin Liu1,2, Huiyun Chang1,2, Junwu Ma1,2, Qingge Xie1,2.
Abstract
The full-length cDNA clone of swine vesicular disease virus HK/70 strain named pSVOK12 was constructed in order to study the antigenicity, replication, maturation and pathogenicity of swine vesicular disease virus. In vitro transcription RNA from pSVOK12 transfected IBRS-2 cells and the recovered virus RNA were isolated and sequenced, then indirect hemagglutination test, indirect immunofluorescence assays, eleectron microscope test, 50% tissue culture infecting dose (TCID50) assays and mouse virulence studies were performed to study the antigenicity and virulence of the recovered virus. The result showed that the infectious clones we obtained and the virus derived from pSVOK12 had the same biological properties as the parental strain HK/70. The full-length infectious cDNA clone, pSVOK12, will be very useful in studies of the antigenicity, virulence, pathogenesis, maturation and replication of SVDV. © Science in China Press 2006.Entities:
Keywords: full-length cDNA; infectious clone; pSVOK12; reverse genetics; swine vesicular disease virus
Year: 2006 PMID： 32214723 PMCID： PMC7089143 DOI： 10.1007/s11434-006-2095-z
Source DB: PubMed Journal: Chin Sci Bull ISSN： 1001-6538