| Literature DB >> 3221039 |
T A Bacon1, F Morvan, B Rayner, J L Imbach, E Wickstrom.
Abstract
Degradation of a synthetic alpha-oligodeoxynucleotide was studied in order to compare its survival with naturally occurring beta-oligodeoxynucleotides in five systems used for antisense hybridization arrest experiments. In contrast to beta-oligodeoxynucleotides, alpha-oligodeoxynucleotides were not detectably degraded over 24 h at 37 degrees C in HeLa cell postmitochondrial cytoplasmic extract or RPMI 1640 with 10% fetal bovine serum, and showed significant survival after 24 h at 37 degrees C in rabbit reticulocyte lysate, fetal bovine serum and human serum.Entities:
Mesh：
Substances：
Year: 1988 PMID： 3221039 DOI： 10.1016/0165-022x(88)90065-6
Source DB: PubMed Journal: J Biochem Biophys Methods ISSN： 0165-022X