UNLABELLED: Fluid administered during resuscitation translocates beneath the burn wound and is considered inert "third space" loss. This study was done to determine whether subeschar tissue fluid (STF) functions as an immunobiologic reservoir. Seven patients with a mean BSA burn of 55% underwent isotonic volume resuscitation and burn treatment with low penetration topical agents. STF was collected at the time of fascial excision. Chemical analysis of STF was similar to serum; bacterial cultures grew no organisms. Ten percent dilutions of STF and burn serum significantly blunted mitogen-induced blastogenic response compared to control serum. STF was significantly more suppressive than burn serum (P less than .03). CONCLUSIONS: 1. STF may act as both an immunologic barrier to microbial clearance in otherwise viable subcutaneous tissue and a reservoir for systemically reabsorbed immunosuppressive factors. 2. In addition to removing dead tissue, fascial excision may prove beneficial because it removes large amounts of immunosuppressive STF.
UNLABELLED: Fluid administered during resuscitation translocates beneath the burn wound and is considered inert "third space" loss. This study was done to determine whether subeschar tissue fluid (STF) functions as an immunobiologic reservoir. Seven patients with a mean BSA burn of 55% underwent isotonic volume resuscitation and burn treatment with low penetration topical agents. STF was collected at the time of fascial excision. Chemical analysis of STF was similar to serum; bacterial cultures grew no organisms. Ten percent dilutions of STF and burn serum significantly blunted mitogen-induced blastogenic response compared to control serum. STF was significantly more suppressive than burn serum (P less than .03). CONCLUSIONS: 1. STF may act as both an immunologic barrier to microbial clearance in otherwise viable subcutaneous tissue and a reservoir for systemically reabsorbed immunosuppressive factors. 2. In addition to removing dead tissue, fascial excision may prove beneficial because it removes large amounts of immunosuppressive STF.
Authors: Jerod Brammer; Gideon Wolf; Scott M Baliban; Jessica C Allen; Myeongjin Choi; Adrienne R Kambouris; Raphael Simon; Gary Fiskum; Wei Chao; Kerri Lopez; Catriona Miller; Nevil J Singh; Alan S Cross Journal: J Burn Care Res Date: 2022-07-01 Impact factor: 1.819