Ali Eskandari 1 , Aziz Mahmoudzadeh 2 , Alireza Shirazi 3 , Farid Esmaely 3 , Carla Carnovale 4 , Mohsen Cheki 5,6 Show Affiliations »
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BACKGROUND AND OBJECTIVE: Cancer incidence is 24% higher in children and young adults exposed to Computed Tomography (CT) scans than those unexposed. Non-repairing of ionizing radiation-induced DNA Double-Strand Breaks (DSBs ) can initiate carcinogenesis. In the present study, we aimed to investigate the radioprotective potential of melatonin against DSBs in peripheral blood lymphocytes of patients undergoing abdomen-pelvis CT examinations . METHODS: This double-blind, placebo -controlled clinical trial was conducted on thirty patients . These patients were divided into two groups; group one (control) patients who have undergone the CT examination received a single oral dose of placebo , while in group two, patients received a single oral dose of 100mg melatonin . In both the groups, blood samples were collected 5-10min before and 30 minutes after the CT examination. The lymphocytes from these samples were isolated and DSBs were analyzed using γH2AX immunofluorescence microscopy. RESULTS: Compared to the control group, the use of melatonin 1h before the CT examination caused a significant reduction in γH2AX-foci , indicating a reduction in DSBs . In addition, no side effect was observed in patients following 100mg melatonin administration. CONCLUSION: For the first time, this study has shown that melatonin has protective effects against radiationinduced genotoxicity in peripheral blood lymphocytes of patients undergoing abdomen-pelvis CT examinations . Therefore, melatonin can be considered as a promising candidate for reducing DSBs in patients undergoing abdomen-pelvis CT examinations . Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.
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BACKGROUND AND OBJECTIVE: Cancer incidence is 24% higher in children and young adults exposed to Computed Tomography (CT) scans than those unexposed. Non-repairing of ionizing radiation-induced DNA Double-Strand Breaks (DSBs ) can initiate carcinogenesis . In the present study, we aimed to investigate the radioprotective potential of melatonin against DSBs in peripheral blood lymphocytes of patients undergoing abdomen-pelvis CT examinations. METHODS: This double-blind, placebo-controlled clinical trial was conducted on thirty patients . These patients were divided into two groups; group one (control) patients who have undergone the CT examination received a single oral dose of placebo, while in group two, patients received a single oral dose of 100mg melatonin . In both the groups, blood samples were collected 5-10min before and 30 minutes after the CT examination. The lymphocytes from these samples were isolated and DSBs were analyzed using γH2AX immunofluorescence microscopy. RESULTS: Compared to the control group, the use of melatonin 1h before the CT examination caused a significant reduction in γH2AX-foci, indicating a reduction in DSBs . In addition, no side effect was observed in patients following 100mg melatonin administration. CONCLUSION: For the first time, this study has shown that melatonin has protective effects against radiationinduced genotoxicity in peripheral blood lymphocytes of patients undergoing abdomen-pelvis CT examinations. Therefore, melatonin can be considered as a promising candidate for reducing DSBs in patients undergoing abdomen-pelvis CT examinations. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.
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Keywords:
DSB; Melatonin; computed tomography; lymphocytes; radiation; γH2AX
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Year: 2020
PMID： 32208125 DOI： 10.2174/1871521409666200324101701
Source DB: PubMed Journal: Anticancer Agents Med Chem ISSN： 1871-5206 Impact factor: 2.505