Antonella Antonelli1, Patryk Szwargulski2,3, Emanuele-Salvatore Scarpa1, Florian Thieben2,3, Grüttner Cordula4, Gianluca Ambrosi1, Loretta Guidi1, Peter Ludewig5, Tobias Knopp2,3, Mauro Magnani1. 1. Department of Biomolecular Sciences, University of Urbino Carlo Bo, Via Saffi 2, 61029, Urbino (PU), Italy. 2. Section for Biomedical Imaging, University Medical Center Hamburg-Eppendorf, Lottestr. 55, 22529, Hamburg, Germany. 3. Institute for Biomedical Imaging, Hamburg University of Technology, Am Schwarzenberg-Campus 3, 21073, Hamburg, Germany. 4. Micromod Partikeltechnologie GmbH, Friedrich-Barnewitz-Str. 4 D-18119, Rostock, Germany. 5. Department of Neurology, University Medical Center Hamburg-Eppendorf, 20251, Hamburg, Germany.
Abstract
Aim: Magnetic particle imaging (MPI) is highly promising for biomedical applications, but optimal tracers for MPI, namely superparamagnetic iron oxide-based contrast agents, are still lacking. Materials & methods: The encapsulation of commercially available nanoparticles, specifically synomag®-D and perimag®, into human red blood cells (RBCs) was performed by a hypotonic dialysis and isotonic resealing procedure. The amounts of superparamagnetic iron oxide incorporated into RBCs were determined by Fe quantification using nuclear magnetic resonance and magnetic particle spectroscopy. Results: Perimag-COOH nanoparticles were identified as the best nanomaterial for encapsulation in RBCs. Perimag-COOH-loaded RBCs proved to be viable cells showing a good magnetic particle spectroscopy performance, while the magnetic signal of synomag-D-COOH-loaded RBCs dropped sharply. Conclusion: Perimag-COOH-loaded RBCs could be a potential tool for MPI diagnostic applications.
Aim: Magnetic particle imaging (MPI) is highly promising for biomedical applications, but optimal tracers for MPI, namely superparamagnetic iron oxide-based contrast agents, are still lacking. Materials & methods: The encapsulation of commercially available nanoparticles, specifically synomag®-D and perimag®, into human red blood cells (RBCs) was performed by a hypotonic dialysis and isotonic resealing procedure. The amounts of superparamagnetic iron oxide incorporated into RBCs were determined by Fe quantification using nuclear magnetic resonance and magnetic particle spectroscopy. Results: Perimag-COOH nanoparticles were identified as the best nanomaterial for encapsulation in RBCs. Perimag-COOH-loaded RBCs proved to be viable cells showing a good magnetic particle spectroscopy performance, while the magnetic signal of synomag-D-COOH-loaded RBCs dropped sharply. Conclusion: Perimag-COOH-loaded RBCs could be a potential tool for MPI diagnostic applications.
Authors: Patrick M Glassman; Elizabeth D Hood; Laura T Ferguson; Zongmin Zhao; Don L Siegel; Samir Mitragotri; Jacob S Brenner; Vladimir R Muzykantov Journal: Adv Drug Deliv Rev Date: 2021-09-29 Impact factor: 15.470