Literature DB >> 32206431

Towards real-time wide-field fluorescence lifetime imaging of 5-ALA labeled brain tumors with multi-tap CMOS cameras.

David Reichert1,2,3, Mikael T Erkkilä1,3, Gerhard Holst4, Nancy Hecker-Denschlag5, Marco Wilzbach5, Christoph Hauger5, Wolfgang Drexler1, Johanna Gesperger1,6, Barbara Kiesel7, Thomas Roetzer6, Angelika Unterhuber1, Georg Widhalm7, Rainer A Leitgeb1,2, Marco Andreana1.   

Abstract

Fluorescence guided neurosurgery based on 5-aminolevulinic acid (5-ALA) has significantly increased maximal safe resections. Fluorescence lifetime imaging (FLIM) of 5-ALA could further boost this development by its increased sensitivity. However, neurosurgeons require real-time visual feedback which was so far limited in dual-tap CMOS camera based FLIM. By optimizing the number of phase frames required for reconstruction, we here demonstrate real-time 5-ALA FLIM of human high- and low-grade glioma with up to 12 Hz imaging rate over a wide field of view (11.0 x 11.0 mm). Compared to conventional fluorescence imaging, real-time FLIM offers enhanced contrast of weakly fluorescent tissue.
© 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.

Entities:  

Year:  2020        PMID： 32206431      PMCID： PMC7075617          DOI： 10.1364/BOE.382817

Source DB:  PubMed          Journal:  Biomed Opt Express        ISSN： 2156-7085            Impact factor:   3.732


  29 in total

Review 1.  Fluorescence lifetime imaging--techniques and applications.

Authors:  W Becker
Journal:  J Microsc       Date:  2012-05-24       Impact factor: 1.758

2.  Fluorescence-guided surgery with 5-aminolevulinic acid for resection of malignant glioma: a randomised controlled multicentre phase III trial.

Authors:  Walter Stummer; Uwe Pichlmeier; Thomas Meinel; Otmar Dieter Wiestler; Friedhelm Zanella; Hans-Jürgen Reulen
Journal:  Lancet Oncol       Date:  2006-05       Impact factor: 41.316

3.  Quantitative fluorescence using 5-aminolevulinic acid-induced protoporphyrin IX biomarker as a surgical adjunct in low-grade glioma surgery.

Authors:  Pablo A Valdés; Valerie Jacobs; Brent T Harris; Brian C Wilson; Frederic Leblond; Keith D Paulsen; David W Roberts
Journal:  J Neurosurg       Date:  2015-07-03       Impact factor: 5.115

4.  Intraoperative confocal microscopy in the visualization of 5-aminolevulinic acid fluorescence in low-grade gliomas.

Authors:  Nader Sanai; Laura A Snyder; Norissa J Honea; Stephen W Coons; Jennifer M Eschbacher; Kris A Smith; Robert F Spetzler
Journal:  J Neurosurg       Date:  2011-07-15       Impact factor: 5.115

5.  Multiphoton excitation fluorescence microscopy of 5-aminolevulinic acid induced fluorescence in experimental gliomas.

Authors:  Sven Rainer Kantelhardt; Heike Diddens; Jan Leppert; Veit Rohde; Gereon Hüttmann; Alf Giese
Journal:  Lasers Surg Med       Date:  2008-04       Impact factor: 4.025

6.  Modulated CMOS camera for fluorescence lifetime microscopy.

Authors:  Hongtao Chen; Gerhard Holst; Enrico Gratton
Journal:  Microsc Res Tech       Date:  2015-10-24       Impact factor: 2.769

7.  In vivo multiphoton tomography and fluorescence lifetime imaging of human brain tumor tissue.

Authors:  Sven R Kantelhardt; Darius Kalasauskas; Karsten König; Ella Kim; Martin Weinigel; Aisada Uchugonova; Alf Giese
Journal:  J Neurooncol       Date:  2016-01-30       Impact factor: 4.130

8.  Simultaneous, label-free, multispectral fluorescence lifetime imaging and optical coherence tomography using a double-clad fiber.

Authors:  Benjamin E Sherlock; Jennifer E Phipps; Julien Bec; Laura Marcu
Journal:  Opt Lett       Date:  2017-10-01       Impact factor: 3.776

9.  Wide-field fluorescence lifetime imaging of cancer.

Authors:  James McGinty; Neil P Galletly; Chris Dunsby; Ian Munro; Daniel S Elson; Jose Requejo-Isidro; Patrizia Cohen; Raida Ahmad; Amanda Forsyth; Andrew V Thillainayagam; Mark A A Neil; Paul M W French; Gordon W Stamp
Journal:  Biomed Opt Express       Date:  2010-08-19       Impact factor: 3.732

10.  Hyperspectral data processing improves PpIX contrast during fluorescence guided surgery of human brain tumors.

Authors:  J J Bravo; J D Olson; S C Davis; D W Roberts; K D Paulsen; S C Kanick
Journal:  Sci Rep       Date:  2017-08-25       Impact factor: 4.379
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  1 in total

Review 1.  5-Aminolevulinic Acid-Induced Protoporphyrin IX Fluorescence Imaging for Tumor Detection: Recent Advances and Challenges.

Authors:  Yoshinori Harada; Yasutoshi Murayama; Tetsuro Takamatsu; Eigo Otsuji; Hideo Tanaka
Journal:  Int J Mol Sci       Date:  2022-06-09       Impact factor: 6.208
  1 in total

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