| Literature DB >> 32206418 |
Panpan Rao1,2,3,4, Long Wang2,3,5,4, Yue Cheng1,2,3, Xi Wang1,2,3,6,7, Haitao Li8, Guoxing Zheng9,6,10, Zile Li9, Chan Jiang1,2,3, Qing Zhou2,3,11, Congxin Huang1,2,3.
Abstract
This study determines whether near-infrared (NIR) light can drive tissue-penetrating cardiac optical control with upconversion luminescent materials. Adeno-associated virus (AAV) encoding channelrhodopsin-2 (ChR2) was injected intravenously to rats to achieve ChR2 expression in the heart. The upconversion nanoparticles (UCNP) NaYF4:Yb/Tm or upconversion microparticles (UCMP) NaYF4 to upconvert blue light were selected to fabricate freestanding polydimethylsiloxane films. These were attached on the ventricle and covered with muscle tissue. Additionally, a 980-nm NIR laser was programmed and illuminated on the film or the tissue. The NIR laser successfully captured ectopic paced rhythm in the heart, which displays similar manipulation characteristics to those triggered by blue light. Our results highlight the feasibility of tissue-penetration cardiac optogenetics by NIR and demonstrate the potential to use external optical manipulation for non-invasive or weakly invasive applications in cardiovascular diseases.Entities:
Year: 2020 PMID： 32206418 PMCID： PMC7075614 DOI： 10.1364/BOE.381480
Source DB: PubMed Journal: Biomed Opt Express ISSN： 2156-7085 Impact factor: 3.732