Wenxin Dai1, Huiying Feng2, Dongwon Lee3. 1. Department of BIN Convergence Technology and Polymer Nano Science and Technology, Chonbuk National University, 664-14, Dukjin, Jeonju 561-756, Republic of Korea; Fourth Ward of Medical Care Center, Hainan Provincial People's Hospital, Haikou 570311, Hainan Province, China. Electronic address: easw212@163.com. 2. Department of BIN Convergence Technology and Polymer Nano Science and Technology, Chonbuk National University, 664-14, Dukjin, Jeonju 561-756, Republic of Korea. 3. Department of BIN Convergence Technology and Polymer Nano Science and Technology, Chonbuk National University, 664-14, Dukjin, Jeonju 561-756, Republic of Korea. Electronic address: leedongwon1022@163.com.
Abstract
PURPOSES: Recently, Methylcrotonoyl-CoA carboxylase 2 (MCCC2) is reported to be involved in tumor formation and progression. However, MCCC2 has nerve been reported in colorectal cancer. In this study, we aimed to investigate the role of MCCC2 in colorectal cancer. METHODS: 118 colorectal cancer and matched adjacent normal tissues were enrolled in this study. The expression level of MCCC2 was measured by quantificational real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC). The clinical significance of MCCC2 and its influence on cell proliferation was further analyzed. RESULTS: Results shown that the mRNA levels of MCCC2 in colorectal cancer tissues were significantly increased compared with those in normal tissues (P < .0001). MCCC2 high-expression was observed in 56.8% colorectal cancer tissues, which was significantly higher than those in normal controls (9.3%, P < .0001). MCCC2 high-expression correlated with tumor size, T stage, lymph node metastasis, distant metastasis, clinical stage and differentiation in colorectal cancer (P < .05). Moreover, MCCC2 high-expression predicted poorer prognosis and could be as an independent prognostic factor. In addition, MCCC2 knockdown significantly inhibited cell proliferation compared with these controls, while MCCC2 overexpression could reverse the effect. CONCLUSION: These data indicate MCCC2 overexpression promotes cell proliferation and predicts poorer prognosis in colorectal cancer.
PURPOSES: Recently, Methylcrotonoyl-CoA carboxylase 2 (MCCC2) is reported to be involved in tumor formation and progression. However, MCCC2 has nerve been reported in colorectal cancer. In this study, we aimed to investigate the role of MCCC2 in colorectal cancer. METHODS: 118 colorectal cancer and matched adjacent normal tissues were enrolled in this study. The expression level of MCCC2 was measured by quantificational real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC). The clinical significance of MCCC2 and its influence on cell proliferation was further analyzed. RESULTS: Results shown that the mRNA levels of MCCC2 in colorectal cancer tissues were significantly increased compared with those in normal tissues (P < .0001). MCCC2 high-expression was observed in 56.8% colorectal cancer tissues, which was significantly higher than those in normal controls (9.3%, P < .0001). MCCC2 high-expression correlated with tumor size, T stage, lymph node metastasis, distant metastasis, clinical stage and differentiation in colorectal cancer (P < .05). Moreover, MCCC2 high-expression predicted poorer prognosis and could be as an independent prognostic factor. In addition, MCCC2 knockdown significantly inhibited cell proliferation compared with these controls, while MCCC2 overexpression could reverse the effect. CONCLUSION: These data indicate MCCC2 overexpression promotes cell proliferation and predicts poorer prognosis in colorectal cancer.
Authors: Eduard Gondáš; Alžbeta Kráľová Trančíková; Eva Baranovičová; Jakub Šofranko; Jozef Hatok; Bhavani S Kowtharapu; Tomáš Galanda; Dušan Dobrota; Peter Kubatka; Dietrich Busselberg; Radovan Murín Journal: Cancers (Basel) Date: 2022-01-24 Impact factor: 6.639