Literature DB >> 3220254

The use of a chromosome integration vector to map erythromycin resistance and production genes in Saccharopolyspora erythraea (Streptomyces erythraeus).

J M Weber1, R Losick.   

Abstract

The thiostrepton-resistance-conferring plasmid pIJ702 was integrated into the ermE region of the chromosome of erythromycin (Er)-producing bacterium Saccharopolyspora erythraea (Streptomyces erythraeus) by single, reciprocal (Campbell) recombination between DNA cloned in the vector and homologous nucleotide sequences in the chromosome. Genetic mapping experiments by conjugational transfer were used to establish that the ErR gene, ermE, was located close to the Er-production loci eryA34 and eryB25.

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Year:  1988        PMID： 3220254     DOI： 10.1016/0378-1119(88)90019-4

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  12 in total

Review 1.  Analysis of an 8.1-kb DNA fragment contiguous with the erythromycin gene cluster of Saccharopolyspora erythraea in the eryCI-flanking region.

Authors:  Andrew R Reeves; Gerhard Weber; William H Cernota; J Mark Weber
Journal:  Antimicrob Agents Chemother       Date:  2002-12       Impact factor: 5.191

2.  Random transposon mutagenesis of the Saccharopolyspora erythraea genome reveals additional genes influencing erythromycin biosynthesis.

Authors:  Andrij Fedashchin; William H Cernota; Melissa C Gonzalez; Benjamin I Leach; Noelle Kwan; Roy K Wesley; J Mark Weber
Journal:  FEMS Microbiol Lett       Date:  2015-10-13       Impact factor: 2.742

3.  Disruption of a rhodaneselike gene results in cysteine auxotrophy in Saccharopolyspora erythraea.

Authors:  S Donadio; A Shafiee; C R Hutchinson
Journal:  J Bacteriol       Date:  1990-01       Impact factor: 3.490

4.  Cloning, characterization, and heterologous expression of the Saccharopolyspora erythraea (Streptomyces erythraeus) gene encoding an EF-hand calcium-binding protein.

Authors:  D G Swan; J Cortes; R S Hale; P F Leadlay
Journal:  J Bacteriol       Date:  1989-10       Impact factor: 3.490

5.  Cloning and sequence analysis of genes involved in erythromycin biosynthesis in Saccharopolyspora erythraea: sequence similarities between EryG and a family of S-adenosylmethionine-dependent methyltransferases.

Authors:  S F Haydock; J A Dowson; N Dhillon; G A Roberts; J Cortes; P F Leadlay
Journal:  Mol Gen Genet       Date:  1991-11

6.  Inhibition of erythromycin synthesis by disruption of malonyl-coenzyme A decarboxylase gene eryM in Saccharopolyspora erythraea.

Authors:  Y J Hsieh; P E Kolattukudy
Journal:  J Bacteriol       Date:  1994-02       Impact factor: 3.490

7.  Cloning vectors, mutagenesis, and gene disruption (ermR) for the erythromycin-producing bacterium Aeromicrobium erythreum.

Authors:  E S Miller
Journal:  Appl Environ Microbiol       Date:  1991-09       Impact factor: 4.792

8.  An erythromycin analog produced by reprogramming of polyketide synthesis.

Authors:  S Donadio; J B McAlpine; P J Sheldon; M Jackson; L Katz
Journal:  Proc Natl Acad Sci U S A       Date:  1993-08-01       Impact factor: 11.205

9.  Identification of a Saccharopolyspora erythraea gene required for the final hydroxylation step in erythromycin biosynthesis.

Authors:  D Stassi; S Donadio; M J Staver; L Katz
Journal:  J Bacteriol       Date:  1993-01       Impact factor: 3.490

10.  Cloning of genes governing the deoxysugar portion of the erythromycin biosynthesis pathway in Saccharopolyspora erythraea (Streptomyces erythreus).

Authors:  J Vara; M Lewandowska-Skarbek; Y G Wang; S Donadio; C R Hutchinson
Journal:  J Bacteriol       Date:  1989-11       Impact factor: 3.490
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