Tao Wu1, Yifei Li1, Baodong Chen1. 1. Department of Neurosurgery, Peking University Shenzhen Hospital, Shenzhen, Guangdong, P.R. China.
Abstract
Background: β-1, 4-galactosyltransferase III (B4GALT3) plays an important role in development tumourigenesis. But its role in human glioblastoma (GBM) is still unknown.Materials and methods: To perform gain-of-function and loss-of-function experiment, we transfected siRNA or vector into GBM cells. CCK-8, EdU staining, transwell, western blot, immunofluorescence staining were applied to perform phenotype and mechanism experiment. GlioVis platform was used to analyze B4GALT3 expression from TCGA datasets. Results: B4GALT3 is upregulated in glioblastoma and predicts poor prognosis. Upregulation of B4GALT3 promotes GBM cell proliferation and invasion while the downregulation of B4GALT3 reduces GBM cell proliferation and invasion. B4GALT3 regulates cell proliferation and invasion via β-catenin and vimentin. B4GALT3 correlates with cell invasion markers in clinical samples. Conclusion: B4GALT3 might be a potential molecular therapeutic target of glioblastoma.
Background: β-1, 4-galactosyltransferase III (B4GALT3) plays an important role in development tumourigenesis. But its role in humanglioblastoma (GBM) is still unknown.Materials and methods: To perform gain-of-function and loss-of-function experiment, we transfected siRNA or vector into GBM cells. CCK-8, EdU staining, transwell, western blot, immunofluorescence staining were applied to perform phenotype and mechanism experiment. GlioVis platform was used to analyze B4GALT3 expression from TCGA datasets. Results:B4GALT3 is upregulated in glioblastoma and predicts poor prognosis. Upregulation of B4GALT3 promotes GBM cell proliferation and invasion while the downregulation of B4GALT3 reduces GBM cell proliferation and invasion. B4GALT3 regulates cell proliferation and invasion via β-catenin and vimentin. B4GALT3 correlates with cell invasion markers in clinical samples. Conclusion:B4GALT3 might be a potential molecular therapeutic target of glioblastoma.