Louis B Polish1, Bobbi Pritt2, Thomas F E Barth3, Bruno Gottstein4, Elise M O'Connell5, Pamela C Gibson6. 1. Division of Infectious Diseases, University of Vermont Larner College of Medicine, Burlington, Vermont, USA. 2. Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA. 3. Institute of Pathology, University of Ulm, Ulm, Germany. 4. Institute of Infectious Diseases, University of Bern, Bern, Switzerland. 5. Helminth Immunology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA. 6. Department of Pathology and Laboratory Medicine, University of Vermont Larner College of Medicine, Burlington, Vermont, USA.
Abstract
BACKGROUND: Echinococcus multilocularis is one of the most severe and lethal parasitic diseases of humans, most often reported in Europe and Asia. Only 1 previous case has been documented in the contiguous United States from Minnesota in 1977. European haplotypes have been identified in carnivores and domestic dogs as well as recently in patients in western and central Canada. METHODS: We used immunohistochemical testing with the monoclonal antibody Em2G11 and a species-specific enzyme-linked immunosorbent assay affinity-purified antigen Em2, as well as COX1 gene sequencing. RESULTS: Using pathology, immunohistochemical staining, specific immunodiagnostic testing, and COX1 gene sequencing, we were able to definitively identify E. multilocularis as the causative agent of our patient's liver and lung lesions, which clustered most closely with the European haplotype. CONCLUSIONS: We have identified the first case of a European haplotype E. multilocularis in the United States and the first case of this parasitic infection east of the Mississippi River. Given the identification of this haplotype in Canada, this appears to be an emerging infectious disease in North America.
BACKGROUND:Echinococcus multilocularis is one of the most severe and lethal parasitic diseases of humans, most often reported in Europe and Asia. Only 1 previous case has been documented in the contiguous United States from Minnesota in 1977. European haplotypes have been identified in carnivores and domestic dogs as well as recently in patients in western and central Canada. METHODS: We used immunohistochemical testing with the monoclonal antibody Em2G11 and a species-specific enzyme-linked immunosorbent assay affinity-purified antigen Em2, as well as COX1 gene sequencing. RESULTS: Using pathology, immunohistochemical staining, specific immunodiagnostic testing, and COX1 gene sequencing, we were able to definitively identify E. multilocularis as the causative agent of our patient's liver and lung lesions, which clustered most closely with the European haplotype. CONCLUSIONS: We have identified the first case of a European haplotype E. multilocularis in the United States and the first case of this parasitic infection east of the Mississippi River. Given the identification of this haplotype in Canada, this appears to be an emerging infectious disease in North America.
Authors: P Deplazes; L Rinaldi; C A Alvarez Rojas; P R Torgerson; M F Harandi; T Romig; D Antolova; J M Schurer; S Lahmar; G Cringoli; J Magambo; R C A Thompson; E J Jenkins Journal: Adv Parasitol Date: 2017-01-20 Impact factor: 3.870
Authors: Thomas F E Barth; Tobias S Herrmann; Dennis Tappe; Lorenz Stark; Beate Grüner; Klaus Buttenschoen; Andreas Hillenbrand; Markus Juchems; Doris Henne-Bruns; Petra Kern; Hanns M Seitz; Peter Möller; Robert L Rausch; Peter Kern; Peter Deplazes Journal: PLoS Negl Trop Dis Date: 2012-10-25