Elena A Dolgikh1, Pyotr G Kusakin1, Anna B Kitaeva1, Anna V Tsyganova1, Anna N Kirienko1, Irina V Leppyanen1, Aleksandra V Dolgikh1,2, Elena L Ilina3, Kirill N Demchenko1,3, Igor A Tikhonovich1,2, Viktor E Tsyganov1,4. 1. All-Russia Research Institute for Agricultural Microbiology, Laboratory of Molecular and Cellular Biology, Saint Petersburg, Russia. 2. Saint Petersburg State University, Department of Genetics and Biotechnology, Universitetskaya embankment 7-9, Saint Petersburg, Russia. 3. Komarov Botanical Institute, Russian Academy of Sciences, Laboratory of Cellular and Molecular Mechanisms of Plant Development, Saint Petersburg, Russia. 4. Saint Petersburg Scientific Center Russian Academy of Sciences, Universitetskaya embankment 5, Saint Petersburg, Russia.
Abstract
BACKGROUND AND AIMS: Recent findings indicate that Nod factor signalling is tightly interconnected with phytohormonal regulation that affects the development of nodules. Since the mechanisms of this interaction are still far from understood, here the distribution of cytokinin and auxin in pea (Pisum sativum) nodules was investigated. In addition, the effect of certain mutations blocking rhizobial infection and subsequent plant cell and bacteroid differentiation on cytokinin distribution in nodules was analysed. METHODS: Patterns of cytokinin and auxin in pea nodules were profiled using both responsive genetic constructs and antibodies. KEY RESULTS: In wild-type nodules, cytokinins were found in the meristem, infection zone and apical part of the nitrogen fixation zone, whereas auxin localization was restricted to the meristem and peripheral tissues. We found significantly altered cytokinin distribution in sym33 and sym40 pea mutants defective in IPD3/CYCLOPS and EFD transcription factors, respectively. In the sym33 mutants impaired in bacterial accommodation and subsequent nodule differentiation, cytokinin localization was mostly limited to the meristem. In addition, we found significantly decreased expression of LOG1 and A-type RR11 as well as KNOX3 and NIN genes in the sym33 mutants, which correlated with low cellular cytokinin levels. In the sym40 mutant, cytokinins were detected in the nodule infection zone but, in contrast to the wild type, they were absent in infection droplets. CONCLUSIONS: In conclusion, our findings suggest that enhanced cytokinin accumulation during the late stages of symbiosis development may be associated with bacterial penetration into the plant cells and subsequent plant cell and bacteroid differentiation.
BACKGROUND AND AIMS: Recent findings indicate that Nod factor signalling is tightly interconnected with phytohormonal regulation that affects the development of nodules. Since the mechanisms of this interaction are still far from understood, here the distribution of cytokinin and auxin in pea (Pisum sativum) nodules was investigated. In addition, the effect of certain mutations blocking rhizobial infection and subsequent plant cell and bacteroid differentiation on cytokinin distribution in nodules was analysed. METHODS: Patterns of cytokinin and auxin in pea nodules were profiled using both responsive genetic constructs and antibodies. KEY RESULTS: In wild-type nodules, cytokinins were found in the meristem, infection zone and apical part of the nitrogen fixation zone, whereas auxin localization was restricted to the meristem and peripheral tissues. We found significantly altered cytokinin distribution in sym33 and sym40 pea mutants defective in IPD3/CYCLOPS and EFD transcription factors, respectively. In the sym33 mutants impaired in bacterial accommodation and subsequent nodule differentiation, cytokinin localization was mostly limited to the meristem. In addition, we found significantly decreased expression of LOG1 and A-type RR11 as well as KNOX3 and NIN genes in the sym33 mutants, which correlated with low cellular cytokinin levels. In the sym40 mutant, cytokinins were detected in the nodule infection zone but, in contrast to the wild type, they were absent in infection droplets. CONCLUSIONS: In conclusion, our findings suggest that enhanced cytokinin accumulation during the late stages of symbiosis development may be associated with bacterial penetration into the plant cells and subsequent plant cell and bacteroid differentiation.
Authors: Anna B Kitaeva; Kirill N Demchenko; Igor A Tikhonovich; Antonius C J Timmers; Viktor E Tsyganov Journal: New Phytol Date: 2015-12-18 Impact factor: 10.151
Authors: Irina V Demina; Pooja Jha Maity; Anurupa Nagchowdhury; Jason L P Ng; Eric van der Graaff; Kirill N Demchenko; Thomas Roitsch; Ulrike Mathesius; Katharina Pawlowski Journal: Front Plant Sci Date: 2019-09-24 Impact factor: 5.753
Authors: Anna B Kitaeva; Artemii P Gorshkov; Evgenii A Kirichek; Pyotr G Kusakin; Anna V Tsyganova; Viktor E Tsyganov Journal: Cells Date: 2021-04-25 Impact factor: 6.600
Authors: Elizaveta S Rudaya; Polina Yu Kozyulina; Olga A Pavlova; Alexandra V Dolgikh; Alexandra N Ivanova; Elena A Dolgikh Journal: Plants (Basel) Date: 2021-12-25