Sawai Boukaew1, Poonsuk Prasertsan2. 1. College of Innovation and Management, Songkhla Rajabhat University, Songkhla, 90000, Thailand. 2. Research and Development Office, Prince of Songkla University, Hat-Yai, Songkhla, 90112, Thailand.
Abstract
AIMS: This study aimed to apply the volatile organic compounds from S. philanthi RL-1-178 (VOCs RL-1-178) as a fumigant to protect soybean seeds against the two aflatoxin-producing fungi in stored soybean seeds. METHODS AND RESULTS: The antifungal bioassay tests on PDA dishes showed that 30 g L-1 wheat seed inoculum of S. philanthi RL-1-178 exhibited total (100 %) inhibition on A. parasiticus TISTR 3276 and A. flavus PSRDC-4. Identification of the VOCs RL-1-178 using GC-MS revealed 39 compounds with the most abundant substances being geosmin (13.75 %) followed by L-linalool (13.55 %), 2-mercaptoethanol (9.71 %) and heneicosane (5.96 %). Comparison on the efficacy of the VOCs RL-1-178 (at 30 g L-1 wheat seed culture) and their four major components (100 µL L-1 each) on the suppression of the two aflatoxin-producing fungi on PDA plates revealed that the VOCs RL-1-178 as well as geosmin, L-linalool, and 2-mercaptoethanol completely inhibited (100 %) mycelial growth while heneicosane showed only 70.7 % inhibition. Use of the VOCs RL-1-178 (30 g L-1 ) as a biofumigant on stored soybean seeds resulted in completely protection (100 %) against the infection as well as completely inhibition on production of aflatoxin (B1 , B2 , and G2 ) (analyzed by HPLC) by the two aflatoxin-producing fungi. CONCLUSIONS: The VOCs RL-1-178 displayed strong inhibitory effects on A. parasiticus TISTR 3276 and A. flavus PSRDC-4 as well as inhibited aflatoxin (B1 , B2 and G2 ) production. SIGNIFICANCE AND IMPACT OF THE STUDY: These findings suggest that the VOCs RL-1-178 can be applied as a biofumigant to control the two aflatoxin-producing fungi on stored seeds products. This article is protected by copyright. All rights reserved.
AIMS: This study aimed to apply the volatile organic compounds from S. philanthi RL-1-178 (VOCs RL-1-178) as a fumigant to protect soybean seeds against the two aflatoxin-producing fungi in stored soybean seeds. METHODS AND RESULTS: The antifungal bioassay tests on PDA dishes showed that 30 g L-1wheat seed inoculum of S. philanthi RL-1-178 exhibited total (100 %) inhibition on A. parasiticus TISTR 3276 and A. flavus PSRDC-4. Identification of the VOCs RL-1-178 using GC-MS revealed 39 compounds with the most abundant substances being geosmin (13.75 %) followed by L-linalool (13.55 %), 2-mercaptoethanol (9.71 %) and heneicosane (5.96 %). Comparison on the efficacy of the VOCs RL-1-178 (at 30 g L-1wheat seed culture) and their four major components (100 µL L-1 each) on the suppression of the two aflatoxin-producing fungi on PDA plates revealed that the VOCs RL-1-178 as well as geosmin, L-linalool, and 2-mercaptoethanol completely inhibited (100 %) mycelial growth while heneicosane showed only 70.7 % inhibition. Use of the VOCs RL-1-178 (30 g L-1 ) as a biofumigant on stored soybean seeds resulted in completely protection (100 %) against the infection as well as completely inhibition on production of aflatoxin (B1 , B2 , and G2 ) (analyzed by HPLC) by the two aflatoxin-producing fungi. CONCLUSIONS: The VOCs RL-1-178 displayed strong inhibitory effects on A. parasiticus TISTR 3276 and A. flavus PSRDC-4 as well as inhibited aflatoxin (B1 , B2 and G2 ) production. SIGNIFICANCE AND IMPACT OF THE STUDY: These findings suggest that the VOCs RL-1-178 can be applied as a biofumigant to control the two aflatoxin-producing fungi on stored seeds products. This article is protected by copyright. All rights reserved.