Chen Wang1, Hong Zhang2, Haijun Liu3, Hongwei Zhang1, Yuqian Bao4, Jianzhong Di5, Cheng Hu6. 1. Department of Bariatric and Metabolic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China. 2. Shanghai Diabetes Institute, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China. 3. Department of General Surgery, Shanghai Fengxian Central Hospital, Affiliated to Southern Medical University, Shanghai, China. 4. Department of Endocrinology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China. 5. Department of Bariatric and Metabolic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China. Electronic address: dijianzhong@sjtu.edu.cn. 6. Shanghai Diabetes Institute, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China. Electronic address: alfredhc@sjtu.edu.cn.
Abstract
BACKGROUND: Alterations in gut microbiota have been associated with improvements in blood glucose due to bariatric surgery. OBJECTIVES: Our aim is to identify specific gut microbiota that contribute to the remission of T2D after RYGB and SG. METHODS: Rats fed a high-fat diet (HFD) were administered a low dose of streptozotocin as T2D models; next, their caecum content was collected 8 weeks after RYGB and SG. We also used case-control of gut microbial profiles of T2D patients and healthy people by collecting the mucosal-luminal interface from the ascending colon. Samples were profiled by 16S rRNA gene sequencing. RESULTS: RYGB and SG reduced weight and improved glucose and insulin tolerance. Principal coordinate analysis showed that SG and, especially, RYGB cause changes in the composition of gut microbiota. We found that family Alcaligenaceae (genus Sutterella) was significantly decreased in the ascending colon of patients with T2D and increased after RYGB surgery in the caecum of T2D rats as shown by a linear discriminant analysis effect size (LEfSe) analysis, with no significant changes after SG. This result might benefit the improvement of glycometabolism. CONCLUSIONS: RYGB can significantly reduce weight, improve glycometabolism and change the composition of the gut microbiota. Sutterella may have beneficial effects on glycometabolism in T2D patients after RYGB.
BACKGROUND: Alterations in gut microbiota have been associated with improvements in blood glucose due to bariatric surgery. OBJECTIVES: Our aim is to identify specific gut microbiota that contribute to the remission of T2D after RYGB and SG. METHODS:Rats fed a high-fat diet (HFD) were administered a low dose of streptozotocin as T2D models; next, their caecum content was collected 8 weeks after RYGB and SG. We also used case-control of gut microbial profiles of T2D patients and healthy people by collecting the mucosal-luminal interface from the ascending colon. Samples were profiled by 16S rRNA gene sequencing. RESULTS: RYGB and SG reduced weight and improved glucose and insulin tolerance. Principal coordinate analysis showed that SG and, especially, RYGB cause changes in the composition of gut microbiota. We found that family Alcaligenaceae (genus Sutterella) was significantly decreased in the ascending colon of patients with T2D and increased after RYGB surgery in the caecum of T2D rats as shown by a linear discriminant analysis effect size (LEfSe) analysis, with no significant changes after SG. This result might benefit the improvement of glycometabolism. CONCLUSIONS: RYGB can significantly reduce weight, improve glycometabolism and change the composition of the gut microbiota. Sutterella may have beneficial effects on glycometabolism in T2D patients after RYGB.
Authors: Anthony A Xu; Luke K Kennedy; Kristi Hoffman; Donna L White; Fasiha Kanwal; Hashem B El-Serag; Joseph F Petrosino; Li Jiao Journal: Nutrients Date: 2022-06-29 Impact factor: 6.706
Authors: Dermot P B McGovern; Jonathan Braun; Jonathan P Jacobs; Maryam Goudarzi; Venu Lagishetty; Dalin Li; Tytus Mak; Maomeng Tong; Paul Ruegger; Talin Haritunians; Carol Landers; Philip Fleshner; Eric Vasiliauskas; Andrew Ippoliti; Gil Melmed; David Shih; Stephan Targan; James Borneman; Albert J Fornace Journal: Genome Med Date: 2022-08-15 Impact factor: 15.266