Hailin Zheng1,2, Oliver Blechert1, Huan Mei1, Liyu Ge1, Jia Liu1, Ye Tao3, Dongmei Li4, G Sybren de Hoog5, Weida Liu1,2,6. 1. Department of Medical Mycology, Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College, Nanjing, China. 2. Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Nanjing, China. 3. Shanghai Biozeron Biotechnology Co., Ltd, Shanghai, China. 4. Department of Microbiology & Immunology, Georgetown University Medical Center, Washington, DC, USA. 5. Center of Expertise in Mycology, Radboud University Medical Center/Canisius Wilhelmina Hospital, Nijmegen, The Netherlands. 6. Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, China.
Abstract
BACKGROUND: Arthroderma uncinatum is a geophilic dermatophyte that occasionally causes superficial infections in humans leading to skin diseases. OBJECTIVES: To better understand the ecology and potential pathogenicity of A uncinatum, we analysed its whole genome. We compared A uncinatum with the genome of the zoophilic dermatophyte Microsporum canis and with the anthropophilic species Trichophyton rubrum. The compared species differ significantly in the frequency of human infection. METHODS: We reported the genome sequence of strain T10 of A uncinatum based on SMRT (single-molecule real-time) technology (PacBio). RESULTS: We obtained a near-complete 23.56 Mb genome, with 7153 predicted gene models and ~20% repetitive sequences. We subsequently determined the specific genetic differences between A uncinatum, M canis and T rubrum. The functional enrichment analysis suggests that A uncinatum is particularly enriched in specific virulence genes. This suggests that the ancestral condition in dermatophytes is with high virulence, which has decreased in the course of evolution to enhance coexistence with animal or human hosts.
BACKGROUND:Arthroderma uncinatum is a geophilic dermatophyte that occasionally causes superficial infections in humans leading to skin diseases. OBJECTIVES: To better understand the ecology and potential pathogenicity of A uncinatum, we analysed its whole genome. We compared A uncinatum with the genome of the zoophilic dermatophyte Microsporum canis and with the anthropophilic species Trichophyton rubrum. The compared species differ significantly in the frequency of humaninfection. METHODS: We reported the genome sequence of strain T10 of A uncinatum based on SMRT (single-molecule real-time) technology (PacBio). RESULTS: We obtained a near-complete 23.56 Mb genome, with 7153 predicted gene models and ~20% repetitive sequences. We subsequently determined the specific genetic differences between A uncinatum, M canis and T rubrum. The functional enrichment analysis suggests that A uncinatum is particularly enriched in specific virulence genes. This suggests that the ancestral condition in dermatophytes is with high virulence, which has decreased in the course of evolution to enhance coexistence with animal or human hosts.
Authors: Alexandra N Myers; Sara D Lawhon; Alison B Diesel; Charles W Bradley; Aline Rodrigues Hoffmann; William J Murphy Journal: PLoS Genet Date: 2022-02-14 Impact factor: 5.917