Celso-Afonso Klein-Junior1, Roberto Zimmer2, Diana-Lina-Bronca Borghetti2, Fernando-Freitas Portella3, Flávia-Carolina Abich1, Daniel-Rodrigo Marinowic4, Keiichi Hosaka5, Eduardo-Galia Reston2. 1. Department of Operative Dentistry, School of Dentistry, Lutheran University of Brazil (ULBRA), Cachoeira do Sul, Rio Grande do Sul, Brazil. 2. Department of Operative Dentistry, School of Dentistry, Lutheran University of Brazil (ULBRA), Canoas, Rio Grande do Sul, Brazil. 3. Institute of Health Sciences, Universidade Feevale, ERS-239 2755, Novo Hamburgo, Rio Grande do Sul, Brazil. 4. Neuroscience Department, Brain Institute, Neuroscience Laboratory, Pontifical Catholic University of Rio Grande do Sul, Rio Grande do Sul, Brazil. 5. Department of Oral Health Science, School of Medical and Dental Science, Tokyo Medical and Dental University, Tokyo, Japan.
Abstract
BACKGROUND: The light-cured calcium hydroxide based cements have incomplete polymerization and unconverted monomers can cause pulp cell damage. The aim of this study was to evaluate the influence of a warm and hot air stream on the cytotoxicity of light-cured calcium hydroxide based cements. MATERIAL AND METHODS: The materials Dycal (conventional cement), Biocal, Hidrox-Cal, and Ultra-Blend Plus (light-cured calcium hydroxide cements) were submitted to cytotoxicity analysis after polymerization, without vs. with previous heat treatment with a warm (37°C) and a hot (60°C) air stream. Following polymerization, cements were maintained in culture medium for 24 hours and 7 days, and subjected to the MTT test. Data were analyzed using analysis of variance (ANOVA) followed by post-hoc Student-Newman-Keuls (<0.05). RESULTS: The results indicated significant differences between the materials according to their composition, i.e., light-cured cements treated with a jet of warm air showed similar cytotoxicity levels to those observed for conventional cement, suggesting that they may be considered alternatives in cases requiring pulp-capping treatment. CONCLUSIONS: Application of a hot air stream reduced cytotoxicity of materials tested. Key words:Dental pulp capping, dental cements, calcium hydroxide, cell survival. Copyright:
BACKGROUND: The light-cured calcium hydroxide based cements have incomplete polymerization and unconverted monomers can cause pulp cell damage. The aim of this study was to evaluate the influence of a warm and hot air stream on the cytotoxicity of light-cured calcium hydroxide based cements. MATERIAL AND METHODS: The materials Dycal (conventional cement), Biocal, Hidrox-Cal, and Ultra-Blend Plus (light-cured calcium hydroxide cements) were submitted to cytotoxicity analysis after polymerization, without vs. with previous heat treatment with a warm (37°C) and a hot (60°C) air stream. Following polymerization, cements were maintained in culture medium for 24 hours and 7 days, and subjected to the MTT test. Data were analyzed using analysis of variance (ANOVA) followed by post-hoc Student-Newman-Keuls (<0.05). RESULTS: The results indicated significant differences between the materials according to their composition, i.e., light-cured cements treated with a jet of warm air showed similar cytotoxicity levels to those observed for conventional cement, suggesting that they may be considered alternatives in cases requiring pulp-capping treatment. CONCLUSIONS: Application of a hot air stream reduced cytotoxicity of materials tested. Key words:Dental pulp capping, dental cements, calcium hydroxide, cell survival. Copyright:
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