Acute mesenteric lymphadenitis in children: findings related to differential diagnosis and hospitalization.

INTRODUCTION: Diagnostic discrimination between acute appendicitis (AA) and mesenteric lymphadenitis (AML) may require more diagnostic tests or great skill after excluding other diagnoses. This study aimed to make a differential diagnosis between AA and AML patients with previous and new parameters and to examine which parameters should be taken into account regarding whether the AML patient should be hospitalized or discharged.
MATERIAL AND METHODS: One hundred and twenty-three AML and 134 AA patients, randomly selected, were included in the study. Demographic, clinical, and laboratory data of all subjects were analyzed. Ultrasonographic and rarely computed tomography examinations evaluating for the enlarged lymph nodes with the shortest diameter in the right lower quadrant of the AML patients were performed. Also, the erect abdominal radiographs (EAR) of AML and AA patients were evaluated.
RESULTS: While there was no statistically significant difference in age or gender, C-reactive protein, white blood cell count, monocyte percentage as well as symptoms between the AA and AML groups (p > 0.05), neutrophil and lymphocyte percentage, appearance of EAR and L/M ratio were significantly different between the two groups (p < 0.05). There was no correlation between the short-axis diameter of the mesenteric lymph node and clinical and laboratory findings in the AML group (p > 0.05).
CONCLUSIONS: Based on the results with ROC curve analysis, we propose to take into account the L/M ratio and the EAR from the parameters in the differential diagnosis between AA and AML in addition to previously reported parameters. Also, we recommend that the same features may be used to decide whether AML patients under observation are hospitalized or not. Copyright:

Introduction

The most common surgical disease in children with acute abdominal pain admitted to the emergency department is acute appendicitis (AA). Diagnostic discrimination between AA and acute mesenteric lymphadenitis (AML) may sometimes be difficult as a result of the physical examination and laboratory studies after excluding other diagnoses such as diarrhea, urinary infection, intussusception, and gastroenteritis [1, 2]. Acute mesenteric lymphadenitis causes right lower quadrant (RLQ) pain as in AA and its etiology may be due to primary (idiopathic) or secondary (infection, malignancy, etc.) reasons [3].

Although AML brings about abdominal pain, it does not cause any abnormalities in many healthy children with enlarged mesenteric lymph nodes. Wang et al. asserted in an ultrasound-guided clinical study that the mesenteric lymph nodes increase with age until 6 years and then decrease. Moreover, they supposed that AML with a short axis diameter larger than 8 mm could be related to abdominal pain [4]. Also, in another study, the short axis diameter of larger than 8 mm and even 10 mm was suggested for the definition of the pathologic mesenteric lymph node in children [5].

The consensus regarding the radiological and laboratory parameters which may be consistent with AML in the literature is still controversial [6, 7]. The most common gastrointestinal complaints in AML are abdominal pain, nausea-vomiting, fever, and loose stools, respectively. The clinical and laboratory results of AA and AML may create uncertainty about what differential diagnosis will be made for most practitioners. While establishing a relationship between the clinic picture and mesenteric lymphadenitis (ML), patients with the short axis diameter of ML greater than 10 mm were excluded from the study in some studies; the others accepted that ML with a short-axis diameter of 8 mm or more constituted AML [5, 8, 9]. Erect abdominal radiographs (EAR) can provide supportive findings in the differential diagnosis of intestinal obstruction or ileus in children [10]. However, no study has made a differential diagnosis by establishing a relationship between EAR and the AML or AA patients’ clinical characteristics.

This study aimed to evaluate the clinical and laboratory parameters of patients with symptomatic AML and to compare them with those of AA patients, and also to further investigate those parameters based on lymph node size. Also, we examined which parameters should be taken into account regarding whether the AML patient should be hospitalized or discharged.

Material and methods

Data of all patients aged 5–16 years who consecutively presented in the Emergency Department and the outpatient clinic of Pediatric Surgery between the dates July 2015 and May 2018 and were diagnosed with AML and AA were retrospectively analyzed. The study was approved by the Institutional Ethics Review Board for Clinical Research of Erzincan Binali Yildirim University (2018, 24/5). Patients with missing data were removed from the study. Demographic, clinical, and laboratory data of all subjects were analyzed. The abdominal ultrasound (US) was performed by a radiologist using the HI VISION Preirus scanner with a curved-array (2–5 MHz) and a linear-array transducer (6–13 MHz; Hitachi Medical Corporation, Japan). Ultrasonographic and rarely computed tomography (CT) examinations evaluating for the enlarged lymph nodes with the shortest diameter in the RLQ of the AML patients were performed. Also, the erect abdominal radiographs of AML and AA patients were evaluated. The US finding of AA was the aperistaltic, incompressible, dilated appendix with > 6 mm diameter. AA was precisely identified according to pathological specimen results after surgery. Symptomatic AML and AA patients were detected according to the discharge summary in which there was found right lower quadrant (RLQ) pain, rebound or tenderness findings of the patients.

Statistical analysis

We performed statistical analysis to for evaluate our results using SPSS Statistics for Windows version 18.0 software (SPSS Inc., USA). When the sample size was less than 50, the Shapiro-Wilk test was used for normality of distribution concerning the data evaluation. Otherwise, the Kolmogorov-Smirnov test was used. For two independent groups, nonparametric tests, such as the Mann Whitney-U test, were used in the case of rejection; otherwise, a t-test was used. The Kruskal-Wallis test was used when the number of groups for the nonparametric data was low. Correlation between categorical variables was analyzed by the χ2 test. Correlation between numeric variables was examined by the Pearson test. We used Levene’s test to assess the equality of variances and a two-sided 95% confidence interval for all measures. Statistical significance was defined at p < 0.05. For the statistically significant result between the groups in the laboratory test used in differential diagnosis, the sensitivity, specificity, and cut-off value were calculated using the receiver operating characteristic (ROC) curve. The area under the curve of 0.50 represented a variable with no differential capacity, and the area of 1.00 indicated a perfect discriminator.

Results

A total of 2680 (100%) patients with abdominal pain were determined. The AA in 134 (5%), AML in 123 (4.5%), constipation in 450 (16.7%), diarrhea in 650 (24.2%), and urinary tract infection in 442 (16.5%) patients were reasons for abdominal pain. Also, incarcerated inguinal hernia, ovarian torsion, testicular torsion, invagination, and diabetic ketoacidosis, etc. in 77 (3.1%) cases, and upper/lower respiratory tract infection in 804 (30%) patients were other reasons for abdominal pain.

One hundred and twenty-three AML patients and 134 AA patients without loss of data were consecutively detected. In 24 (9.7%) out of 247 patients with intensive intestinal gas or obesity, CT was used instead of USG because the diagnosis and differential diagnosis could not be reliably performed. The final diagnosis of AML or AA was made with the US and CT examination. Also, AA diagnosis was confirmed by the patient’s pathology and operative report, and the postoperative diagnosis of all preoperative AA patients was the same. There was no significant difference in age or gender between the two groups or within each group (p > 0.05; Table I).

Table I

Comparison of age and gender between AA and AML groups and within each group

Parameter	N (%)	Age, mean ± SD	P-value (age)	P-value (gender)
AA:			0.72	0.65
Male	64 (52)	9.71 ±2.54
Female	59 (48)	9.87 ± 2.22
Total	123 (100)	9.76 ± 2.43
AML:			0.50	0.73
Male	69 (52)	9.80 ±2.81
Female	65 (48)	9.48 ±2.08
Total	134 (100)	9.70 ±2.58
AA vs. AML	257 (100)	9.73 ±2.51	0.84	0.86

AA – group of acute appendicitis, AML – group of acute mesenteric lymphadenitis.

RLQ tenderness, abdominal guarding, and rebound tenderness, which were the findings of RLQ pain in patients, were present in the examination of all patients excluding five AA and nine AML patients who were admitted for only vomiting and AA and AML were detected on ultrasound examination, respectively (Table II).

Table II

Comparison of AA and AML groups’ data including erect abdominal radiography, symptoms and complete blood count results

Parameter	AA (n = 123)	AML (n = 134)	P-value
CRP (mean ± SD) [mg/dl]	10.56 ±4.21	10.21 ±4.56	0.53
WBC (mean ± SD) [× 103/µl]	10.80 ±2.66	10.28 ±2.23	0.18
Neutrophils (N) %	76.13 ±3.66	58.15 ±10.89	0.00
Lymphocytes (L) %	10.76 ±3.66	32.27 ±11.58	0.00
Monocytes (M) %	5.70 ±1.80	6.64 ±5.07	0.15
L/M ratio	2.06 ±0.86	5.52 ±1.63	0.00
Symptoms, n (%):	123 (100)	134 (100)	0.66
1	69 (56)	63 (47)
2	49 (40)	62 (46)
3	5 (4)	9 (7)
EAR, n (%):	123 (100)	134 (100)	0.02
A	76 (62)	52 (39)
B	42 (34)	51 (38)
C	5 (4)	31 (23)

AA – group of acute appendicitis, AML– group of acute mesenteric lymphadenitis. Symptoms: 1 – only RLQ pain, 2 – RLQ pain and vomiting, 3 – only vomiting. EAR – Erect abdominal radiograph: A – gas-fluid level present, B – gas-fluid level is absent but abundant gas exists, C – normal appearance.

None of the AML patients were detected to have wall thickening of any bowel segment. According to retrospective data in both groups, symptoms were divided into three variables: 1, only RLQ pain; 2, RLQ pain and vomiting; 3, only vomiting. From the complete blood count results, white blood cells (WBC; reference < 12.5 × 103/µl), neutrophil (N), lymphocyte (L) and monocyte (M) percentage, as well as L/M ratio were obtained. C-reactive protein (CRP; reference < 10 mg/dl) records of all patients were collected. Erect abdominal radiographs (EAR) were taken from the radiological records, and they were separated into three groups: A – gas-fluid level present; B – the gas-fluid level is absent, but abundant gas exists; C – normal appearance. Patients who did not meet these parameters were not included in the study. As shown in Table II, there was no statistically significant difference between the two groups except for N and L percentage, EAR, and L/M ratio during the first evaluation (Table II, p < 0.05). When the ROC curve for parameters in the discrimination of groups was plotted, the parameters with the largest area under the curve (AUC) were N and L percentage, L/M ratio, and EAR (Figure 1). Cut-off value, sensitivity and specificity for N, L percentage and L/M ratio were 80.9, 10% and 89%; 48.7, 20% and 94%; as well as 8.95, 89% and 97%, respectively.

Figure 1

ROC curve for parameters in the discrimination of groups (AA and AML)

Observation status was conferred for AML patients considered not ill enough to be fully admitted but not well enough to go home especially due to vomiting and pain. To decide which patient should be admitted to the hospital the same parameters as above were used. AML patients under observation were reevaluated after about 8 h (range: 4–8 h) of symptomatic treatment was applied especially for pain and nausea-vomiting. US findings of the hospitalized AML patients were usually a peristaltic, compressible appendix with 6–8 mm diameter. As the cause of large diameter of the appendix together with some AML patients could be a doubtful case of appendicitis, other findings were evaluated. We reevaluated the new EAR and current symptoms of them. As shown in Table III, there was no statistically significant difference between the hospitalized and discharged AML patients except for EAR (Figure 2), symptoms and L/M ratio (p < 0.05). When the ROC curve for parameters in the discrimination of groups was plotted, the parameter with the largest area under the curve was only L/M ratio (Figure 3). Cut-off value, sensitivity and specificity for L/M ratio were 8.95, 89% and 97%, respectively.

Table III

Comparison of hospitalization and discharge characteristics in AML patients under observation

Parameter	Hospitalized AML*	Discharged AML	P-value
WBC (mean ± SD) [×103/µl]	10.42 ±1.51	10.26 ±2.33	0.29
Neutrophils (N) (%)	58.21 ±14.23	58.09 ±10.28	0.78
Lymphocytes (L) (%)	38.38 ±7.23	11.13 ±1.04	0.62
Monocytes (M) (%)	7.39 ±2.37	7.39 ±1.95	0.46
L/M ratio	6.48 ±1.90	5.09 ±1.55	0.02
Symptoms, n (%):	20 (100)	114 (100)	0.00
1	0 (0)	99 (87)
2	12 (60)	0 (0)
3	8 (40)	15 (13)
EAR, n (%):	20 (100)	114 (100)	0.004
A	18 (90)	34 (30)
B	2 (10)	49 (43)
C	0 (0)	31 (27)

AA – group of acute appendicitis, AML – group of acute mesenteric lymphadenitis. Symptoms: 1 – Only RLQ pain, 2 – RLQ pain and vomiting, 3 – only vomiting. EAR – erect abdominal radiograph; A – gas-fluid level present, B – gas-fluid level is absent but abundant gas exists, C – normal.

AML patients not fully recovering from nausea-vomiting and pain after 8 hours of symptomatic treatment.

Figure 2

Abdominal erect radiographs of three distinct AML patients. A – Normal X-ray of a discharged patient. B – Gas-fluid levels present in the X-ray of a hospitalized patient. C – A hospitalized patient with small gas pockets and small gas-fluid levels (A, B and C patients with a normal appendix on the US examination)

Figure 3

ROC curves for indicating whether patients with AML should be hospitalized or discharged

On the ultrasound examination, we detected a cluster of three or more enlarged lymph nodes in the RLQ and mesentery of the ileocaecal region or the anterior to the psoas, and also measured the short-axis diameter of the largest lymph node in all patients with AML. We found that there was no significant correlation between all parameters of AML patients and the short axis diameter of the largest lymph node (p > 0.05); Table IV). As shown in Figure 4 and Table III, the number of patients with a short axis diameter of the largest lymph node less than 8 mm was 118 (88%). These results indicated that the parameters constituting the clinical condition were not affected by the lymph node size.

Table IV

Correlation of short-axis diameter of mesenteric lymph node with clinical and laboratory findings in the AML group

A			B
AML group Parameters		A&B correlation P-value	Short axis diameter of the largest lymph node [mm]
WBC (mean ± SD) [× 103/µl]	10.28 ±2.23	0.08	(mean ± SD; 5.62 ± 2.26)(rank: 3–12 mm)
CRP (mean ± SD) [mg/dl]	10.21 ±4.56	0.16	Diameter	N	Percent
Lymphocytes (L) (%)	32.27 ±11.58	0.42	3.00	18	13.4
Monocytes (M) (%)	6.64 ±5.07	0.40	4.00	30	22.4
L/M ratio	5.52 ±1.63	0.90	5.00	39	29.1
Symptoms, n (%):	134 (100)	0.44	6.00	12	9.0
1	63 (47)		7.00	6	4.5
2	62 (46)		8.00	13	9.7
3	9 (7)		9.00	3	2.2
EAR, n (%):	134 (100)	0.48	10.00	7	5.2
A	52 (39)		11.00	3	2.2
B	51 (38)		12.00	3	2.2
C	31 (23)		Total	134	100.0

AML – acute mesenteric lymphadenitis. Symptoms: 1 – Only RLQ pain, 2 – RLQ pain and vomiting, 3 – only vomiting. EAR – erect abdominal radiograph: A – gas-fluid level present, B – gas-fluid level is absent but abundant gas exists, C – normal appearance.

Figure 4

Histogram showing distribution of a short axis diameter measurement of the largest lymph node according to AML patient numbers

Discussion

When looking at the literature, there was generally no significant difference in age (range: 9–13) or gender in studies comparing AML and AA patients [8, 11]. Conversely, there was a study that found significant differences in age between AA and AML patients (male vs. female: 10.3 vs. 7.9 years) [7]. This difference may be due to the fact that the regions where these studies were carried out are different (Israel, Latvia and India). In our study, there was no statistically significant difference in age (mean: 9.73 ±2.51 years) or gender between the AA and AML groups. Also, there was no significant difference regarding age or gender among the patients of the AML group. The same characterization was also present in the AA group (Table I, p > 0.05).

The AML has a clinical presentation that can mimic AA, and this dilemma may lead to a negative exploration in the operations performed with AA pre-diagnosis [6, 12]. Various clinical and laboratory parameters have been used in studies to cope with this difficulty. N to L count ratio in the diagnosis of AA was established and this parameter was accepted as a positive predictor in favor of AA. Moreover, Gross et al. suggested that lymphocytosis is a positive predictor in favor of AML regarding the differential diagnosis between AML and AA [6, 13]. Similarly, in our study, N percentage was higher in AA and L percentage was higher in AML patients. Differently, in our study, the L/M ratio was significantly higher in the AML group than that of the AA group (Table II, p < 0.001). The percentage of M alone was not different between the two groups. In a study performed for AA and AML differential diagnosis, the monocyte chemoattractant protein-1 (MCP 1) ratio was found not to be different between these two groups. In this study, although the MCP 1 ratio was high in AA, there was no statistically significant difference between the two groups [11]. This result was compatible with our work regarding the M percentage in the discrimination of both groups (p = 0.15). The CRP and WBC rates were higher in favor of AA in a recent study and this result was statistically significant [6]. There was, however, no significant difference in WBC and CRP between the two groups in our study (p > 0.05). As shown in Table II, when L and N are within normal limits, the L/M ratio seems to be more reliable when making a differential diagnosis between AA and AML. In a recent study, higher values of N/L and M/L ratio demonstrated greater possibilities for bacterial infection and low probabilities for viral infection [14]. Rothrock et al. found that initial misdiagnosis in childhood appendicitis included gastroenteritis and upper respiratory tract infection in the percentage of 60% [8, 15]. M/L and N/L ratio can help the practitioner to differentiate these diseases since AML has the same confusion and condition in the differential diagnosis of AA, and because the viral agents are often considered in the etiology of these involved diseases regarding discrimination of AA. When considering the above pieces of evidence, we tried to make a differential diagnosis between AA and AML, taking into account the L/M ratio instead of the M/L ratio, and to facilitate the diagnosis of AML, which is more likely to be viral by etiology.

Even though the Alvarado score and the pediatric appendicitis score described by Samuel were efficiently used for the diagnosis of AA [7, 16, 17], some authors in their prospective studies reported that both scores could be useful parameters for the diagnosis of AA; however, they concluded that these scores were insufficient to determine whether surgical intervention is required or not [18-20]. In other words, it has been revealed that differential diagnosis cannot be made with current symptoms. According to the parameters we collected from the archive records, abdominal pain percentage was higher in the AA group, while nausea-vomiting was higher in the AML group, but these were statistically insignificant in the differential diagnosis (p = 0.66). In this context, complementary radiological examinations were needed for differential diagnosis and correct treatment. Because of this, CT examination was recommended in cases where the differential diagnosis between AA and AML was not possible with the US examination [7].

We traditionally used EARs [21] in patients in whom we could not make a differential diagnosis with US. Thus, with the CT examination, patients were not exposed to more radiation in the first assessment. When the appendix could not be visualized due to abundant intestinal gas on the US examination, or it could not be determined in any area of the RLQ, we first looked at the patient’s L/M ratio. We observed the patients with an L/M ratio of 5 and above. We then used the EAR to assess intestinal stability. We used CT examination (about 9.7%, 24 out of 257 patients) in patients with an L/M ratio less than 5. Although there was no diarrhea in the AML patients under observation (in all of the AML patients hospitalized and in some of the subjects discharged) their EARs had gas-liquid levels or small gas pockets (Figures 2 B, C). AML patients not fully recovering from nausea-vomiting and abdominal pain after 8 h of symptomatic treatment were hospitalized by looking at their new EARs and L/M ratio. While vomiting was more frequent in the hospitalized AML patients, abdominal pain was more frequent in discharged patients, and this clinical difference was statistically significant (p < 0.001). Moreover, while 90% of the hospitalized AML patients had EARs with gas-fluid levels, 70% of the discharged AML patients had EARs with normal gas distribution and small air pockets, which was statistically significant as well (p = 0.004).

As shown in Table II, there was no significant correlation between the short-axis diameter of the mesenteric lymph node and clinical or laboratory findings in the AML group (p > 0.05). However, it has been stated in the literature that especially abdominal pain occurred in AML patients with the short-axis diameter of the mesenteric lymph node over 8 or 10 mm. Also, it has been supposed that a cluster of three or more lymph nodes measuring more than 5 mm along their short axis in AML was the pathologic condition [3, 4, 7, 22]. In our study, we found that abdominal pain and nausea-vomiting occurred at different rates independently of the short-axis diameter of the mesenteric lymph node (Figure 4, Table IV). In a recent study, there were no significant differences between laboratory and clinical parameters and lymph node size [6]. We have also shown that there is no correlation between EAR results and lymph node size.

In the ROC curve analysis, the L/M ratio was the most specific and the most sensitive parameter we used when making a differential diagnosis between AA and AML groups (Figure 1). We found that the previously reported percentage of L [6] was less sensitive and specific. We detected that the same result presented in the ROC curves for indicating whether patients with AML could need to be hospitalized or discharged (Figure 2).

Since our study was retrospective, we could not obtain the body temperature precisely from the records. However, it was previously reported that high fever did not show any significant difference in the differential diagnosis [7]. Unlike adults, discriminating between children with and without appendicitis usually requires more effort. In other words, a 100% reliable laboratory test and the clinical feature have not yet been reported in the differential diagnosis. There is a need for more specific studies regarding the inflammatory process for AA and AML. For example, cytokines such as IL-1β, IL-1ra, and IL-6 used for the clinical approach to ulcerative colitis relapse can be investigated in AA and AML [12, 23, 24]. Colonoscopy may be used for higher diagnostic reliability of AML in children as in a recent study in which esophagogastroduodenoscopy was performed in children with chronic abdominal pain [25]. Thus, in an acute stage of AML, colonoscopy may detect the intestinal changes which could be associated with AML by the same logical approach. In differential diagnosis, anamnestic factors are also important; for example, when the abdominal pain of a patient with chronic renal failure is questioned, it is malpractice to try to make the AA or AML discrimination instead of performing dialysis-related tests immediately [26].

Another limitation is that, as in Gross’s study [6], the analysis of results based on three categories may not be as realistic as a prospective study for the parameter under the heading “symptoms” associated with the physical examination. Although the EAR is used less frequently in clinical practice, it is still used, for example, in the diagnosis of intestinal perforation [27]. In addition to the approaches mentioned above, we made a differential diagnosis of diseases such as intestinal perforation or intussusception via EAR.

In conclusion, when a child with abdominal pain is admitted to the emergency department, a complete blood count should be performed after the physical examination. Even if the WBC is within standard limits, the L/M ratio seems to be reliable for initial differential diagnosis between AA and AML. If it is not found that there is no appendicitis sign in the US, after this stage, it would be diagnosed in some diseases other than AA and AML using EAR, and thus patients will not be exposed to more radiation with CT. Computed tomography examination, which is one of the best technological facilities, is recommended in cases where the differential diagnosis between AA and AML is not possible with the USG examination or EAR made after USG examination.

Based on these results, we propose to take into account the L/M ratio and EAR from the parameters in the differential diagnosis between AA and AML in addition to previously reported parameters. Also, we recommend that the same features be used to determine whether AML patients under observation are hospitalized or not. But there is a need for prospective studies on the accuracy and effectiveness of all the parameters we used.

Conflict of interest

The authors declare no conflict of interest.