Efficacy of a Single Intra-Articular HYMOVIS ONE Injection for Managing Symptomatic Hip Osteoarthritis: A 12-Month Follow-Up Retrospective Analysis of the ANTIAGE Register Data.

PURPOSE: The use of ultrasound (US) guidance has allowed hip osteoarthritis to be treated with intra-articular (IA) injections. HYMOVIS ONE (HYADD4-G) is a new hyaluronic acid (HA) derivative product with unusual characteristics, and it has been used with good results in knee osteoarthritis (OA). This study assessed the efficacy and safety of a single HYMOVIS ONE injection in patients affected by symptomatic hip OA. PATIENTS AND METHODS: This post-marketing cohort study assessed data from the ANTIAGE Register. Inclusion criteria were age ≥40 years, symptomatic hip OA (Kellgren-Lawrence grade I-III) of ≥1-year duration, and ≥12 months follow-up. All patients received a single HYMOVIS ONE (32 mg/4 mL) injection at baseline. Values for 10-cm visual analogue scale (VAS) pain scores, the Lequesne index, and nonsteroidal anti-inflammatory drug (NSAID) consumption were evaluated at 6 and 12 months. Adverse events were also recorded.
RESULTS: The included patients (n = 198) consisted of 42.5% women, with a mean (± SD) age at baseline of 62 (± 14.2) years and a mean (± SD) body mass index of 26.3 (± 2.5). The mean (SD) Lequesne index and VAS pain scores at baseline were 11.5 (± 4.6) and 6.4 cm (± 2.2), respectively. All groups exhibited statistically significant reductions at all time points compared to baseline. At 12 months, the VAS pain score was reduced by 17.2%, the Lequesne index by 33.7%, and NSAID consumption by 41.7%.
CONCLUSION: Our study supports the clinical efficacy and safety of a single HYMOVIS ONE injection for managing symptoms in patients with hip OA, confirming previous data on the use of HYMOVIS as a background therapy in the management of knee osteoarthritis.

Introduction

Hip osteoarthritis (OA) is one of the major causes of pain and disability in the western population. Pain consequent to hip OA may significantly hinder the patient’s ability to perform daily activities, and disablement may be so impairing that it leads to social isolation. The disease prevalence is approximately 17% in white males and 9% in white women over 60 years of age.1 Treatment aims to relieve pain and to preserve or restore joint mobility in milder cases, and when the disease stage is more severe, to delay total hip replacement (THR)2 given the fact that THR, even if 90% effective,3,4 is associated with substantial adverse effects and relevant mortality rates.5,6

Pharmacological treatments include acetaminophen as a first option, followed by nonsteroidal anti–inflammatory drugs (NSAIDs) for non-responding patients, to be taken with a gastroprotective agent or a selective COX-2 inhibitor. When NSAIDs are ineffective, patients may be treated with opioid analgesics.7 Possibly, patients will take medications for their remaining lifespan, and long-term effects and the economic burden of medications should be the subject of careful consideration.8 Therefore, alternative options for managing hip OA symptoms are actively being investigated, as they may reduce analgesic consumption.

Among these, viscosupplementation (VS) is receiving increasing consideration as a valid option for managing symptoms of mild to moderate hip OA, given the results of recent randomized clinical trials2,9 and recommendations of expert consensuses.10,11 Hip VS is normally performed under ultrasound (US) or fluoroscopy guidance. This has replaced the use of anatomic landmarks, reducing morbidity and increasing precision in intra-articular (IA) delivery.12,13

Among viscosupplementation agents, hyaluronic acid (HA) has been extensively studied because it is a natural component of synovial fluid (SF). Here, its concentration varies between 0.5 and 4 mg/mL and its molecular weight (MW) ranges from 2 to 10 MDa.14,15 Under dynamic loading, weak non-ionic forces between HA molecules confer to HA unique non-Newtonian rheological properties, including shear thinning and reduction of viscosity,16–18 which confers outstanding visco-elasticity, shock absorption, and lubricating properties to the SF, particularly during high shear or compression conditions.19 Joint arthropathies, including OA, are associated with a reduction of the molecular weight and concentration of hyaluronan in the synovial fluid,20 causing SF rheological properties to worsen, and therefore contributing further to OA progression.15

Novel injectable HA formulations often present a modified HA chain. Modifications are introduced to modulate the rheological properties and resorption kinetics of the formulation and increase the effectiveness in the clinical setting.21,22 A HA alkyl-derivative, non-chemically cross-linked-based formulation (HYADD 4-G, Fidia Farmaceutici, Abano Terme, Italy) has recently entered the market as a viscosupplementation agent. It is a highly viscoelastic injectable hydrogel whose peculiar rheological properties are derived from the presence of hexadecyl (C-16) hydrophobic chains that facilitate its aggregation even at low aqueous concentrations.23,24 Priano and colleagues25 have shown that 24 mg/3 mL high molecular-weight viscoelastic hyaluronan (HYMOVIS/HYADD4-G) injections reduced Western Ontario and McMaster Universities Osteoarthritis (WOMAC) scores and NSAID/acetaminophen consumption for at least 6 months in 698 patients with mild to severe knee OA (Kellgren–Lawrence grades II–IV) who underwent two consecutive infiltrations 1 week apart. In a subpopulation of 106 patients, efficacy against pain lasted approximately 12 months.25

Previously, Bisicchia et al26 conducted a single-center, single-blind, prospective randomized controlled clinical study with 1-year follow-up, and showed that 2 (24 mg/3 mL) HYMOVIS injections did not have significantly higher side effects when compared to corticosteroid injections and provided better short-term control of symptoms in patients with mild to moderate knee osteoarthritis. Our group has extensively studied the effect of HA viscosupplementation in managing symptoms of hip OA, using non-modified HA within the 1.5–2.0 MDa range injected under US guidance.27–32

In a recent prospective, post-market seven-year follow-up cohort study whose data were collected from the Associazione Nazionale per la Terepia Intra Articolare dell’Anca con Guida Ecografica (ANTIAGE) register, we found that hip OA patients who received non-modified HA injections at least every 6 months showed, at all time points and compared to baseline, a statistically significant improvement of Lequesne index, pain visual analogue scale (VAS), NSAID intake, and global medical and patient assessments.32 Modifications made to HA to improve its rheology and resorption kinetics may, in principle, modulate the clinical response of patients to IA injection; consequently, the optimal therapeutic protocol for a given HA formulation should be investigated on a case-by-case basis. As the ANTIAGE register contains records concerning hip OA patients who were treated by a single (32 mg/4 mL) HYMOVIS ONE IA injection, and as single-injection protocols may be of special clinical interest because of their intrinsic enhanced safety and patient acceptability, we decided to retrospectively analyze the records to assess the safety and performance profile of this modified injectable HA formulation, in relation to this administration regimen.

Materials and Methods

Study Design

This is a multicentre, observational, retrospective analysis of patient register data regarding subjects affected by mild to moderate hip OA, who were treated with a single (32 mg/4 mL) HYMOVIS ONE IA injection and were followed up for at least 12 months at 6 Italian orthopaedic/rheumatology centres, namely the Rheumatology Unit at the S. Pietro FBF Hospital, Rome (the referral centre); the Osteo-Articular Diagnostic Procedures Operative Unit, at the Medical and Surgical Science and Neuroscience Department, Siena; the Physical and Rehabilitation Medicine Department at University of Rome Tor Vergata, Rome; the Orthopaedics and Traumatology Department at Lucca Hospital, Lucca; the Rheumatology Department at the Gaetano Pini Institute, Milan; and the Physical and Rehabilitation Medicine Department, Napoli University, Naples.

Records were retrieved from the ANTIAGE register, a prospectively built database holding deidentified patient data, which is described elsewhere.33 Data extraction and analysis were approved by the ethics committee of each centre.

Study Population

Records were included if related to patients (both males and females) who were ≥40 years of age; had BMI < 30; had an X-ray-confirmed diagnosis of mild to moderate, Kellgren and Lawrence34 (KL) grade I to III hip OA; showed moderate to severe pain, corresponding to 40 to 80 mm VAS scores at the affected hip; were treated with one (32 mg/4 mL) HYMOVIS ONE IA injection and followed up for at least 12 months.

Patients included in the study had their VAS pain scores, Lequesne index scores, and nonsteroidal anti-inflammatory drug (NSAID) consumption assessed at baseline (before the IA injection) and 6 and 12 months thereafter. Records were excluded if patients showed any of the following: severe OA (KL grade IV); concomitant use of oral anticoagulant therapy, severe comorbidities (eg, rheumatologic disease, low back pain, femoral head osteonecrosis), significant varus or valgus deformity requiring surgical correction, inflammatory diseases that might affect joints, positive anamnesis for sepsis or subacute infection in any joint, relevant lymphatic stasis at the treated hip, hypersensitivities to any components of HA-based injection, or treatment with HA or HA derivatives in the previous 6 months or with IA steroids in the previous 3 months.

Procedures

Radiological evaluations according to the KL hip OA scale were made on a non-weight-bearing X-ray, and were taken no more than 6 months prior to the start treatment date. Pain was evaluated using a 100-mm VAS score.

IA injections were performed under US guidance12 according to an anterosuperior approach that has been perfected and standardized over time by the authors.13,33 The approach utilizes an US transducer together with a sterile bioptic target device while patients are placed supine with the hip at 15–20° internal rotation, and the hip joint is scanned according to an anterior parasagittal approach, lateral to the femoral vessels. The transducer is aligned with the long axis of the femoral neck, comprising the acetabulum and the femoral head. The injection is performed by inserting a 20-gauge (9 cm) spinal needle into the biopsy guide. Using biopsy real-time guidance software, the needle is advanced into the anterior capsular recess, at the level of the femoral head and, when in contact with it, is retracted by 1 mm before starting the injection. The injection into the articular space was verified by real-time monitoring (direct visualization of viscous fluid or air bubbles) and power Doppler imaging (flow signals in the intra-articular recess). The colour Doppler view also enabled the physician to avoid blood vessels.

Objectives and Endpoints

The primary study objective was to assess the duration of symptom relief and functional improvement following a single HYMOVIS ONE injection, when measured using the VAS pain and Lequesne index scores as well as NSAID consumption. The secondary study objective was to investigate the safety of the HYMOVIS ONE administration regimen, in terms of frequency and type of adverse events recorded.

Statistical Analysis

Record screening and inclusion was carried out at the single-centre level. Included records were then transferred to the referral centre (Rheumatology Unit, S. Pietro FBF Hospital). Here, they were re-checked according to the inclusion and exclusion criteria. The data were extracted from records, and homogeneity of patient distribution among different centres concerning age, gender, body mass index (BMI), and KL and OA scores at baseline was assessed using parametric or non-parametric analysis of variance tests, according to the results of normality checks. Normality of data was checked using Shapiro–Wilks tests.

Patients demographics as well as adverse event type and frequency were described by means of descriptive statistics that is, absolute or relative frequencies (%) for discrete variables and as mean ± standard deviation (SD) for continuous ones, as they all were found to have a normal distribution. To investigate if treatment with a single HYMOVIS ONE injection had a statistically significant effect on the performance endpoints of interest, the VAS and Lequesne scores collected at each control visit were compared to those at baseline by means of t-tests for paired data. Statistical test results were regarded as significant when p<0.05.

Results

Records were analysed for 198 patients (114 men and 84 women) with a mean age of 62 ± 14.2 years. Characteristics of the study cohort at baseline are shown in Table 1. Most patients (90.9%) were afflicted with KL OA grade II or III and were on average overweight, with their BMI being 26.3 ± 2.5.

Table 1

Characteristics of the Study Cohort at Baseline

Patients (n)	198
Men	114 (57.5%)
Women	84 (42.5%)
Age, years, mean (SD)	62 (± 14.2)
BMI, mean (SD)	26.3 (± 2.5)
Weight, kg, mean (SD)	73.7 (± 10.6)
Height, cm, mean (SD)	169.7 (± 7.2)
Lequesne index, mean (SD)	11.5 (± 2.5)
Pain VAS, mean (SD)	6.4 (± 2.2)
NSAID intake (days/months mean, (SD))	16.3 (± 4.6)
Age classes
<65 y.o.	95 (48.0%)
>65 y.o.	103 (52.0%)
Hip affected
Right	85 (42.9%)
Left	74 (37.4%)
Bilateral	39 (19.7%)
Kellgren–Lawrence radiological index
Grade I	18 (9.1%)
Grade II	79 (39.9%)
Grade III	101 (51.0%)

Patients experienced a significant reduction of all scores under consideration compared to baseline (Table 2). At 6 months, compared to baseline, VAS pain decreased by 15.6%, and at 12 months, it was still reduced by a similar amount (17.2%) (Figure 1). Both variations were statistically significant compared to baseline (p<0.05). The Lequesne index underwent a 19.0% reduction after 6 months, and at 12 months, had decreased by 33.7% (Figure 2). At 6 months from the first injection, NSAID consumption decreased by 21.8%, and at 12 months, had decreased by 41.7% (Figure 3). For both the Lequesne index and NSAID consumption, the reductions at 6 months compared to baseline, at 12 months compared to baseline, and at 12 months compared to 6 months were statistically significant (p<0.05 in all cases).

Table 2

Pain VAS, Lequesne Index, and NSAID Consumption Variation Over Time

	Baseline	6 Months	%	12 Months	%
Pain VAS	6.4 ± 2.2	5.4 ± 2.0* (p=0.002)	−15.6	5.3 ± 1.9* (p=0.004)	−17.2
Lequesne index	11.5 ± 4.6	9.3 ± 3.7* (p=0.012)	−19.0	7.6 ± 2.4* (p=0.008)	−33.7
NSAIDs consumption(days/month)	16.3 ± 6.0	12.8 ± 3.2* (p=0.007)	−21.8	9.5 ± 2.1* (p=0.009)	−41.7

Notes: All values are given as the mean ± SD. *Significant difference compared to pre-treatment (p<0.05); % variations are calculated from baseline.

Figure 1

Decrease in pain over time, measured through a visual analogue scale (VAS). The decrease is statistically significant (p<0.05) from baseline to 6 months and 12 months.

Figure 2

Decrease in Lequesne index over time. The decrease is statistically significant (p<0.05) both from baseline to 6 and 12 months, and between 6 and 12 months.

Figure 3

Decrease in NSAID consumption over time, measured in days/months. The decrease is statistically significant (p<0.05) both from baseline to 6 and 12 months, and between 6 and 12 months.

No systemic or severe local side effects were reported after the injection. In 5 out of 198 injections (2.5%), there was a sensation of pain lasting from several hours to a few days that spontaneously regressed without requiring medical intervention.

Discussion

Several international scientific societies have developed recommendations concerning the use of VS in the form of IA HA to manage symptoms of hip OA, indicating that it is a viable option as an adjuvant or, in less severe cases, an alternative to pharmacologic treatment.7,35 Yet, VS by HA injection is still under scrutiny by the scientific and clinical community, as it has been suggested that the data from controlled trials should be added with evidence from the real world setting and with longer follow-up.27

Furthermore, HA in different commercially available HA-based formulations may present different chemical modifications that have been introduced to improve the formulation rheology, resorption kinetics, and clinical effectiveness. Thus, one should assume that different HA-based formulations display different safety and effectiveness profiles, until proven otherwise. The matter is of clinical importance concerning safety and its consequences in clinical practice. The invasiveness of IA injections, in fact, may affect negatively the patient’s adherence to the therapy course, resulting in the subject who rejects VS and relies once more only on medicine, and thus exposes him/herself to a greater risk of being affected by short- and long-term side effects. Thus, among HA formulations having equal effectiveness, one might prefer those involving a smaller number of IA injections. Less injections also implies a reduced risk for the patient, even when side effects of the formulations being compared are not different concerning their severity.

The results of the present study show that a single HYMOVIS ONE injection effectively managed symptoms of hip OA decreasing pain and reducing the need of NSAIDs intake over the 12 months of follow-up. The population of patients included had not been prospectively selected specifically to carry out this investigation and, even if reduced in size, may therefore be regarded as representative of the heterogeneous population that may be encountered, and treated, in common clinical practice.

The results of the present study are consistent with those observed by our group within the first year of treatment in a prospective, seven-year follow-up study where more than one IA injection of non-modified (60 mg/4 mL) HA32 was administered to 1022 hip OA patients to manage their OA symptoms. In this study, patients were categorized by age, gender, and body mass index (BMI), and the effect of IA injections was evaluated for each group by measuring how their Lequesne index, pain VAS, NSAID intake, and global medical and patient assessments varied over time. All groups showed a statistically significant reduction, at all time points compared to baseline, of all scores under assessment, with some differences concerning patients older than 70 years, whose scores were systematically higher at baseline than those of other age classes. VAS pain improvement at 1 year was lower (−21%) in patients older than 70 and greater (−39%) in other age classes. The decrease in the Lequesne index was similar in patients older than 70 (−26%) and in other age classes (−28%); NSAID consumption decreased in patients older than 70 years by −27% and by −33% in other age classes.

The results of the present study are also consistent with recent preliminary results by Rando et al36 concerning 30 subjects between 45 and 65 years of age who practiced tennis or amateur cycling for no less than 10 years and were affected by KL grade II or III hip OA. They were subjected to cycles of two IA US-guided HYMOVIS (24 mg/3 mL) injections 2 weeks apart from each other, repeated every 3 or 4 months, as required, during the 24 months of the study. All endpoints considered, including the Heidelberg Sports Activity Score (HAS) scale and the Copenhagen Hip and Groin Outcome Score (HAGOS) assessing symptoms, activity, limitations, participation restrictions, and quality of life, significantly improved over the study course, starting from the first IA injection.

The results of the present study are also comparable with those achieved using HYMOVIS to manage symptoms of knee OA patients. Priano et al25 analysed the retrospective data concerning 698 patients who received two infiltrations of (24 mg/3 mL) HYMOVIS, 1 week apart. The mean WOMAC scores were reduced by 56.3% from baseline. NSAID/acetaminophen use 2 times/week (48.8% of patients at baseline) was substantially reduced after 1 month and was 19.6% after 6 months. The effect on resting pain was rapid, strong, and lasting, as evident by a 56.8% reduction from baseline at 6 months and 53.6% at 12 months. Pain upon moving was reduced by 47.4% after 6 months and 46.0% after 12 months.

Although the results we have obtained are consistent with those just reported, they were achieved through a single HYMOVIS ONE injection.

The possibility that a reduced number of HYMOVIS injections may be as safe and effective as multiple ones has already been suggested by other authors. In 2010, Pavelka et al37 randomized 439 knee KL II or III stage OA patients either to 2 or 3 injections of 24 mg/3 mL HYMOVIS. Their results showed that there were no clinically relevant and/or statistically significant differences between the two treatment groups with regard to the primary outcome, that is, pain during a 50-ft (15 m) walk test, which was measured by VAS at all control visits, with the final reduction at 6 months being, in the two-injection group, 58.9%.

Recently, Bernetti and Santilli38 showed that a single (32 mg/4 mL) HYMOVIS ONE IA injection is safe and effective in improving VAS, WOMAC, Knee injury and Osteoarthritis Outcome Score (KOOS), and biomechanical (gait analysis) scores over 12 months in 23 active patients. They emphasize how this result may be relevant because these patients, given their young age, may show low adherence to treatments involving multiple injections. The effectiveness of single HYMOVIS ONE IA injections to manage hip OA symptoms, as well as OA symptoms that affect other joints, should therefore be the subject of further, carefully designed investigations. The reasons that may be at the basis of the positive results that may be achieved through a single HYMOVIS ONE IA injection possibly consist in its enhanced rheological properties. The HYMOVIS HA molecule, in fact, consists of lateral hexadecyl (C-16) hydrophobic chains that facilitate its aggregation even at low aqueous concentrations,23 creating a mobile reticulum (MO.RE.). Because of the rheological properties of this formulation, it is an optimal viscosupplementation and shock absorbing agent.24

In a prospective, open-pilot study twelve patients affected by symptomatic hip OA underwent a single 2 mL Hylan G-F 20 (Synvisc, Sanofi Aventis - a high molecular weight, cross-linked hyaluronan derivative) ultrasound-guided intra-articular injection. Most subjects experienced a sustained improvement in their Lequesne score, the average reduction being 15% after 3 months.39 Pain reduction was statistically significant, compared to baseline, both 1 and 3 months after the injection, yet no significant difference was observed between the 1- and 3-month time points. A single Synvisc injection, therefore, did not provide a continuous improvement as it happened in the present study.39 The use of Hylan G-F 20 has been associated with a case of post-injection arthritis.40 The frequency and severity of adverse events observed in the present study were similar to those observed when analyzing data from the ANTIAGE registry related to 1906 patients who received 4002 injections with five different HA-based products according to our procedure, who experienced no systemic adverse events, and local pain reactions with a frequency ranging from 2.2% to 3.6%.33 Future studies with larger samples will have to confirm our data.

In a systematic review41 analysing 20 trials with a total of 3034 patients with knee osteoarthritis, the authors concluded that there was limited evidence showing a superior effect of Hylan G-F 20 compared to low molecular weight hyaluronic acids (LMWHA) in pain relief in the period from 2 to 3 months post injections. The authors speculated that this difference could be caused by a different resorption kinetic of the two formulations; accordingly, even HYMOVIS, given its molecular weight and characteristic rheology that may also affect its resorption kinetic, might perform better than LMWHA. Future controlled studies should be aimed to investigate if different formulations, including HYMOVIS ONE, Hylan G-F 20 and LMWHA, are equally safe and effective when administered according to a single injection protocol in prospectively recruited hip OA patients. Further studies should address the question if a single HYMOVIS injection is as effective as more injections, or if it is differently safe and effective than one or more injections of other HA-based formulations. Such studies would address one of the main limitations of the present study, that is the fact it lacks a control group.

Future studies should also address if single-injection protocols are more effective in specific subgroups of patients, when these are graded according to the most common scores used in clinical practice or according to other baseline characteristics, such as gender, BMI and OA severity. Also, HA IA single-injection protocols have never been studied over time periods longer than 12 months. Accordingly, further studies should be targeted at investigating the long-term response of hip OA patients when they undergo prolonged single-injection HYMOVIS ONE treatments.

Conclusions

A single HYMOVIS ONE injection was safe and effective for managing symptoms of patients affected by Kellgren–Lawrence grade I to III hip osteoarthritis, over a 12-month time period, in real-life conditions. Prospective studies with adequate sample size and design are needed to determine the criteria for the selection of patients that can benefit most by a single-injection protocol as well as to determine the optimal injection volume according to their characteristics and clinical signs at presentation.