The influence of variables on the bioavailability of heavy metals during the anaerobic digestion of swine manure.

The speciation of heavy metals, besides the total concentrations, urgently need to be considered when assessing the eco-toxicity and the bioavailability of heavy metals in environment. This paper aims to investigate the distribution and chemical speciation (e.g. the acid extractable fraction (F1), the reducible fraction (F2), the oxidizable fraction (F3), and the residual fraction (F4)) of heavy metals during the anaerobic digestion process of swine manure. The majority of six heavy metals from the manure was located in biogas residue in the order of decreasing concentration Zn > Cu > Ni > As > Pb > Cd. The transformation of heavy metals among four fractions was observed during the digestion process, and the change of bioavailable fraction of Zn, Cu, Ni, Cd, As and Pb were 9.71%, -6.04%, -19.24%, 13.62%, -16.48% and -7.22%, respectively. The heat map of correlation coefficients and the stepwise linear regressions model were established to describe the correlation between the bioavailability of the metals and the given digestion variables to predict the influence of the selected variables on the bioavailability of heavy metals. The variations of heavy metal bioavailable fractions are attributed to three key digestion variables, NH4+-N concentration, CH4% in biogas daily yield and pH. These results provide a new perspective for analysis and control of heavy metals during the anaerobic digestion process.