Erhan Durceylan1, Ebubekir Aksu1, Hacer Boztepe1, Çiğdem Çengelli2, Evrim Çiftçi Yılmaz3, Emine Dündar Kasapoğlu3, Kevser Erol2, Muammer Cumhur Sivrikoz1. 1. Department of Thoracic Surgery, Eskişehir Osmangazi University Health Application and Research Hospital, Eskişehir, Turkey. 2. Department of Medical Pharmacology, Eskişehir Osmangazi University Health Application and Research Hospital, Eskişehir, Turkey. 3. Department of Medical Pathology, Eskişehir Osmangazi University Health Application and Research Hospital, Eskişehir, Turkey.
Abstract
BACKGROUND: This study aims to investigate the protective effect of melatonin on lung damage induced by one-lung ventilation in a rat model. METHODS: A total of 20 healthy, Sprague-Dawley male rats were randomized into two equal groups as control (n=10) and melatonin groups (n=10). The control group underwent 60 min of one-lung ventilation, followed by 30 min of two-lung ventilation. In the melatonin group, the rats were administered 10 mg/kg melatonin intraperitoneally 10 min before the start of the experiment. At the end of both ventilation periods, tissue samples were obtained from the lungs of the control and melatonin groups for biochemical analysis and histopathological examinations. Tissue superoxide dismutase, malondialdehyde, and tumor necrosis factor-alpha levels were measured. Lung tissue samples were examined based on the presence and amount of alveolar congestion, intra-alveolar bleeding, and leukocyte and lymphocyte infiltration. RESULTS: At the end of the study, lung tissue malondialdehyde (3.8±0.9 vs. 1.8±0.8 μM; p<0.001) and tumor necrosis factor-alpha levels (47.2±15.0 vs. 21.8±7.2 pg/mL; p<0.001) of the melatonin group were found to significantly decrease, compared to the control group. Superoxide dismutase levels of the melatonin group increased at the end of both ventilation periods, and the increase at the end of one-lung ventilation was found to be statistically significant (0.6±0.2 vs. 1.3±0.7 U/mL; p<0.05). Histopathological examination demonstrated that the tissue damage was less in the melatonin group. There was a significant decrease in the alveolar congestion in this group (p=0.0401). Although other histopathological parameters decreased in the melatonin group, no significant difference was found. CONCLUSION: Our study results demonstrate that melatonin has protective effects on the lung damage induced by one-lung ventilation both at biochemical and histopathological levels in rats.
BACKGROUND: This study aims to investigate the protective effect of melatonin on lung damage induced by one-lung ventilation in a rat model. METHODS: A total of 20 healthy, Sprague-Dawley male rats were randomized into two equal groups as control (n=10) and melatonin groups (n=10). The control group underwent 60 min of one-lung ventilation, followed by 30 min of two-lung ventilation. In the melatonin group, the rats were administered 10 mg/kg melatonin intraperitoneally 10 min before the start of the experiment. At the end of both ventilation periods, tissue samples were obtained from the lungs of the control and melatonin groups for biochemical analysis and histopathological examinations. Tissue superoxide dismutase, malondialdehyde, and tumor necrosis factor-alpha levels were measured. Lung tissue samples were examined based on the presence and amount of alveolar congestion, intra-alveolar bleeding, and leukocyte and lymphocyte infiltration. RESULTS: At the end of the study, lung tissue malondialdehyde (3.8±0.9 vs. 1.8±0.8 μM; p<0.001) and tumor necrosis factor-alpha levels (47.2±15.0 vs. 21.8±7.2 pg/mL; p<0.001) of the melatonin group were found to significantly decrease, compared to the control group. Superoxide dismutase levels of the melatonin group increased at the end of both ventilation periods, and the increase at the end of one-lung ventilation was found to be statistically significant (0.6±0.2 vs. 1.3±0.7 U/mL; p<0.05). Histopathological examination demonstrated that the tissue damage was less in the melatonin group. There was a significant decrease in the alveolar congestion in this group (p=0.0401). Although other histopathological parameters decreased in the melatonin group, no significant difference was found. CONCLUSION: Our study results demonstrate that melatonin has protective effects on the lung damage induced by one-lung ventilation both at biochemical and histopathological levels in rats.
Authors: Antonio Carrillo-Vico; Russel J Reiter; Patricia J Lardone; Juan Luis Herrera; Rafael Fernández-Montesinos; Juan Miguel Guerrero; David Pozo Journal: Curr Opin Investig Drugs Date: 2006-05
Authors: Carmen Rodriguez; Juan C Mayo; Rosa M Sainz; Isaac Antolín; Federico Herrera; Vanesa Martín; Russel J Reiter Journal: J Pineal Res Date: 2004-01 Impact factor: 13.007