| Literature DB >> 32174710 |
Ritesh Kumar1, Suresh Chandra Yadav2, Sandeep Kumar1, Neeraj Dilbaghi1.
Abstract
A rapid flow-through immunoassay using protein A labeled gold nanoparticles (GNPs) for the qualitative detection of trypanosomosis in equine serum samples was developed. The flow-through device consisted of antigen-coated nitrocellulose membrane fixed on absorbent pads. The GNPs based detection probe was used as the colored marker. The assay can be employed for the detection of Trypanosoma specific antibodies at 14 days post-infection in the infected pooled serum samples of equines. The assay can be conducted in < 5 min. The results of the flow-through assay were comparable with the methods like ELISA, dot blot assay and lateral flow assay. The developed assay showed high efficiency for detection of anti-Trypanosoma antibodies in equine sera and can be used for quick screening of serum samples under field conditions. © Indian Society for Parasitology 2019.Entities:
Keywords: Flow-through assay; Gold nanoparticles; Rapid immunoassay; Trypanosoma evansi; Trypanosomosis
Year: 2019 PMID： 32174710 PMCID： PMC7046882 DOI： 10.1007/s12639-019-01166-8
Source DB: PubMed Journal: J Parasit Dis ISSN： 0971-7196