Citrus aurantium L. bloom essential oil nanoemulsion: Synthesis, characterization, cytotoxicity, and its potential health impacts on mice.

This research was performed to synthesize the Citrus aurantium L. bloom essential oil nanoemulsion (CABE-NE) and investigate its cytotoxic and apoptotic impacts on human lung (A549 cells), and further, the effects of CABE-NE on mice health parameters was determined. The obtained results demonstrated that C. aurantium bloom contained 1.2 ± 0.16% of essential oil with linalyl acetate, limonene, and α-terpineol as major compounds. The CABE-NE possessed particle size of 76.9 ± 6.11 nm, PDI of 0.19, and zeta potential of -43.5 mV. The CABE-NE indicated the cytotoxicity against A549 cells with the IC50 value of 152 µg/ml. The CABE-NE induced the overexpression of Cas-3 and triggered the apoptotic cells death. The mice gavaged daily with CABE-NE at the concentrations of 10 and 20 mg/kg body weight for 30 days did not show any remarkable histopathological alteration in the liver and kidney while exhibited enhancement in the jejunum morpho-structural architecture and hepatic antioxidant redox potential. PRACTICAL APPLICATIONS: According to the results, the produced CABE-NE drug delivery system could be considered as a promising alternative to prevent lung cancer progression and it provides a new way to enhance the therapeutic value of the plant phytochemicals.