Eloy Girela-López1, Cristina M Beltran-Aroca2, Ignacio Jimena3, Pablo Pérez-Jorge4, Valentin Ramos-Medina5, Ignacio Ruz-Caracuel3, James R Gill6, Jose Peña-Amaro3. 1. Sección de Medicina Legal y Forense, Facultad de Medicina y Enfermería, Universidad de Córdoba, Avda Menéndez Pidal s/n, 14004, Córdoba, Spain. 2. Sección de Medicina Legal y Forense, Facultad de Medicina y Enfermería, Universidad de Córdoba, Avda Menéndez Pidal s/n, 14004, Córdoba, Spain. cristinabeltran@uco.es. 3. Departamento de Ciencias Morfológicas, Facultad de Medicina y Enfermería, Sección de Histología, Universidad de Córdoba, 14004, Córdoba, Spain. 4. Sección de Patología Forense, Instituto de Medicina Legal y Ciencias Forenses de Córdoba, 14011, Córdoba, Spain. 5. Sección de Patología Forense, Instituto de Medicina Legal y Ciencias Forenses de Málaga, 29071, Málaga, Spain. 6. Office of the Chief Medical Examiner, Farmington, CT, 06032, USA.
Abstract
PURPOSE: Previous experiments in rats have indicated that there are histological changes in skeletal muscle in drowning deaths; these changes include muscle fibers that contain ragged red fibers (RRF). The purpose of this study was to examine whether these changes also occur in humans. METHODS: Histologic and histochemical examinations of three muscles (diaphragm, pectoralis, and psoas) were performed on 24 cadavers with three different causes of death: 8 drowning, 8 hanging, and 8 sudden cardiac disease. Muscle samples were stained with hematoxylin-eosin, MGT, nicotinamide adenine dinucleotide-tetrazolium reductase, succinate dehydrogenase, ATPase, and acid phosphatase via standard staining procedures. RESULTS: There were statistically significant differences in the detection of RRFs in these cohorts. Additionally, several other cytoarchitectural changes (whorled and core-like fibers) were observed in the diaphragm in the drowning cohort and to a lesser extent in the hangings. These structural abnormalities were not observed in the sudden cardiac disease deaths, thus suggesting a common mechanism for the production of these muscular changes that is not shared in the cardiac death group. The mechanism is most likely intense hypoxia and mechanical trauma of the respiratory muscles in the setting of active blood circulation with intense muscle contraction. CONCLUSIONS: Our results confirmed that there are histological changes in the diaphragm in drownings and, to a lesser extent, in hangings.
PURPOSE: Previous experiments in rats have indicated that there are histological changes in skeletal muscle in drowning deaths; these changes include muscle fibers that contain ragged red fibers (RRF). The purpose of this study was to examine whether these changes also occur in humans. METHODS: Histologic and histochemical examinations of three muscles (diaphragm, pectoralis, and psoas) were performed on 24 cadavers with three different causes of death: 8 drowning, 8 hanging, and 8 sudden cardiac disease. Muscle samples were stained with hematoxylin-eosin, MGT, nicotinamide adenine dinucleotide-tetrazolium reductase, succinate dehydrogenase, ATPase, and acid phosphatase via standard staining procedures. RESULTS: There were statistically significant differences in the detection of RRFs in these cohorts. Additionally, several other cytoarchitectural changes (whorled and core-like fibers) were observed in the diaphragm in the drowning cohort and to a lesser extent in the hangings. These structural abnormalities were not observed in the sudden cardiac disease deaths, thus suggesting a common mechanism for the production of these muscular changes that is not shared in the cardiac death group. The mechanism is most likely intense hypoxia and mechanical trauma of the respiratory muscles in the setting of active blood circulation with intense muscle contraction. CONCLUSIONS: Our results confirmed that there are histological changes in the diaphragm in drownings and, to a lesser extent, in hangings.
Entities:
Keywords:
Cytoarchitectural changes; Drowning; Hanging; Ragged red fibers; Skeletal muscle; Sudden cardiac death
Authors: M D Pérez-Cárceles; S del Pozo; A Sibón; J A Noguera; E Osuna; M A Vizcaya; A Luna Journal: Forensic Sci Int Date: 2011-08-27 Impact factor: 2.395