Jing-Yu Guo1, Yu-Kun Wang1, Bo Lv2, Hong Jin3. 1. Department of stomatology, the second affiliated hospital of mudanjiang medical university, mudanjiang, HeiLongjiang, 157000, P.R. China. 2. Eye 3 division of red flag hospital of mudanjiang medical university, mudanjiang, HeiLongjiang, 157000, P.R. China. 3. College of stomatology, of mudanjiang medical university, mudanjiang, HeiLongjiang, 157000, P.R. China.
Abstract
BACKGROUND: Aberrant miRNAs expression regulates the occurrence and progression of a variety of cancers, including oral squamous cell carcinoma (OSCC). This study aims to illustrate the potential effects of miR-454/nuclear receptor subfamily 3 group C member 2 (NR3C2) on the biological behaviors of OSCC cells. METHODS: GEO database was applied to detect and analyze the expression of miR-545 and NR3C2 in OSCC tissues. Two OSCC cell lines including CAL27 and Tca-83 were utilized to determine the function of miR-454/NR3C2 on OSCC cells biological behaviors. miR-454 and NR3C2 expression were regulated by miR-454 mimic/inhibitor and pcDNA3.1-NR3C2/si-NR3C2, respectively. Cells biological behaviors were evaluated by cell proliferation, colony formation and transwell assays RESULTS: The data collected from GEO database indicated that miR-454 expression was up-regulated in OSCC tissues, however, the expression of NR3C2 assumed a downward trend. In vitro experiments, the expression trend of miR-454 in OSCC cell lines was consistent with that of the trend in tissues, and the OSCC cells growth and movement abilities significantly decreased after miR-454 depletion. Through co-transfection experiments, we explored that the abilities of OSCC cell proliferation, colony formation, invasion and migration obviously reduced after miR-454 depletion, but thees phenomens were mitigated to some extent after NR3C2 silencing. CONCLUSION: The study illustrates that miR-454 acts as an active regulator to facilitate OSCC cells growth, colony formation, invasion and migration by targeting NR3C2, which may afford a novel perspective and possibility for the targeted treatment of OSCC. This article is protected by copyright. All rights reserved.
BACKGROUND: Aberrant miRNAs expression regulates the occurrence and progression of a variety of cancers, including oral squamous cell carcinoma (OSCC). This study aims to illustrate the potential effects of miR-454/nuclear receptor subfamily 3 group C member 2 (NR3C2) on the biological behaviors of OSCC cells. METHODS: GEO database was applied to detect and analyze the expression of miR-545 and NR3C2 in OSCC tissues. Two OSCC cell lines including CAL27 and Tca-83 were utilized to determine the function of miR-454/NR3C2 on OSCC cells biological behaviors. miR-454 and NR3C2 expression were regulated by miR-454 mimic/inhibitor and pcDNA3.1-NR3C2/si-NR3C2, respectively. Cells biological behaviors were evaluated by cell proliferation, colony formation and transwell assays RESULTS: The data collected from GEO database indicated that miR-454 expression was up-regulated in OSCC tissues, however, the expression of NR3C2 assumed a downward trend. In vitro experiments, the expression trend of miR-454 in OSCC cell lines was consistent with that of the trend in tissues, and the OSCC cells growth and movement abilities significantly decreased after miR-454 depletion. Through co-transfection experiments, we explored that the abilities of OSCC cell proliferation, colony formation, invasion and migration obviously reduced after miR-454 depletion, but thees phenomens were mitigated to some extent after NR3C2 silencing. CONCLUSION: The study illustrates that miR-454 acts as an active regulator to facilitate OSCC cells growth, colony formation, invasion and migration by targeting NR3C2, which may afford a novel perspective and possibility for the targeted treatment of OSCC. This article is protected by copyright. All rights reserved.