Literature DB >> 321699

Pronase pretreatment of tissue sections enhances sensitivity of the unlabelled antibody-enzyme (PAP) technique.

H Denk, T Radaszkiewicz, E Weirich.   

Abstract

Treatment of dewaxed sections of formalin-fixed paraffin embedded material with 0.1% pronase for 15 min at 37 degrees C prior to the performance of the unlabelled antibody-enzyme (PAP) procedure greatly enhanced its sensitivity. This was shown by detection of immunoglobulin within plasma cells in chronic gastritis specimens.

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Year:  1977        PMID： 321699     DOI： 10.1016/0022-1759(77)90027-8

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  35 in total

1.  Immunohistologic detection of immunoglobulins in malignant lymphomas and its value in histopathologic diagnosis.

Authors:  T Radaszkiewicz; H Denk
Journal:  Virchows Arch A Pathol Anat Histol       Date:  1979-02-09

2.  Immunocytochemical demonstration of intermediate filament cytoskeleton proteins in human endocrine tissues and (neuro-) endocrine tumours.

Authors:  H Hoefler; H Denk; E Lackinger; G Helleis; J M Polak; P U Heitz
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1986

3.  Glucose oxidase as label in histological immunoassays with enzyme-amplification in a two-step technique: coimmobilized horseradish peroxidase as secondary system enzyme for chromogen oxidation.

Authors:  W D Kuhlmann; P Peschke
Journal:  Histochemistry       Date:  1986

4.  Detection of cytomegalovirus antigens and DNA in tissues fixed in formaldehyde.

Authors:  N M Jiwa; A K Raap; F M van de Rijke; A Mulder; J J Weening; F E Zwaan; T H The; M van der Ploeg
Journal:  J Clin Pathol       Date:  1989-07       Impact factor: 3.411

5.  Lectin affinity electrophoresis in a yolk sac tumour in the vagina with yolk sac tumour-type glycoform of alpha fetoprotein.

Authors:  R Yamamoto; K Taketa; Y Ebina; Y Cho; H Hareyama; N Sakuragi; S Makinoda; K Kobayashi; S Nishi; S Fujimoto
Journal:  J Clin Pathol       Date:  1997-10       Impact factor: 3.411

6.  Distribution of TPA and cytokeratins in gastrointestinal carcinomas as revealed by immunohistochemistry.

Authors:  A Beham; W Weybora; E Lackinger; H Denk; V Björklund; B Björklund
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1986

7.  Detection of peroxisomes in human liver and kidney fixed with formalin and embedded in paraffin: the use of catalase and lipid beta-oxidation enzymes as immunocytochemical markers.

Authors:  J A Litwin; A Völkl; J Stachura; H D Fahimi
Journal:  Histochem J       Date:  1988-03

8.  The use of proteolytic enzymes to improve immunoglobulin staining by the PAP technique.

Authors:  B L Mepham; W Frater; B S Mitchell
Journal:  Histochem J       Date:  1979-05

9.  Pregnancy specific beta 1-glycoprotein and alpha 2-pregnancy associated globulin in tumours of the female genital tract.

Authors:  H Ludwig; G Tatra
Journal:  Klin Wochenschr       Date:  1981-07-15

10.  The use of radioimmune assay in investigating reagents to be used in the immunocytochemical localization of hepatitis B surface antigen in immune complexes in the kidney of patients with membranous nephropathy and Australia antigenaemia.

Authors:  S Wolfe-Coote
Journal:  Histochem J       Date:  1983-09
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