| Literature DB >> 32161771 |
Renu A Kowluru1, Ghulam Mohammad1, Nikhil Sahajpal1.
Abstract
[This corrects the article DOI: 10.1186/s40662-019-0167-9.].Entities:
Year: 2020 PMID: 32161771 PMCID: PMC7047402 DOI: 10.1186/s40662-020-00178-3
Source DB: PubMed Journal: Eye Vis (Lond) ISSN: 2326-0254
Fig. 2Homocysteine metabolizing machinery in diabetic retinopathy. Retinal microvessels were employed to determine CBS (a) gene transcripts by q-RTPCR, (b) protein expression by Western blotting, using β-actin as a housekeeping gene and loading protein, respectively, and (c) enzyme activity by measuring fluorescence at 368 nm excitation and 460 nm emission wavelengths. Values obtained from nondiabetic controls are considered as 100%. Gene transcripts of (d) MTHFR and (e) CSE were quantified by q-RTPCR using β-actin as a housekeeping gene. Data are represented as mean ± SD, obtained from retinal microvessels from 6 to 8 nondiabetic and 7–8 diabetic retinopathy donors. *p < 0.05 vs. nondiabetic donors