Maren Kasper1, Karoline Walscheid1,2, Björn Laffer1,2, Dirk Bauer1, Martin Busch1, Karin Loser3, Thomas Vogl4, Thomas Langmann5, Gerd Ganser6, Thomas Rath7, Arnd Heiligenhaus1,2. 1. Department of Ophthalmology, Ophtha-Lab at St. Franziskus Hospital, Münster, Germany. 2. University of Duisburg-Essen, Essen, Germany. 3. Department of Dermatology, Experimental Dermatology and Immunobiology of the Skin University of Münster, Münster, Germany. 4. Department of Immunology, University of Münster, Münster, Germany. 5. Experimental Immunology of the Department of Ophthalmology, University of Cologne, Cologne, Germany. 6. Department of Pediatric Rheumatology, St. Josef-Stift Sendenhorst, Sendenhorst, Germany. 7. Department of Nephrology, Immunology and Osteology of St. Franziskus Hospital, Münster, Germany.
Abstract
Purpose: To analyze circulating immune cells in patients with anterior uveitis (AU) associated to axial spondyloarthritis (SpA), or juvenile idiopathic arthritis (JIA). Methods: Venous blood samples were collected from healthy controls (n = 16), and either SpA (n = 19) or JIA (n = 23) patients with associated anterior uveitis (AU) during active flare, or after ≥3 months of inactivity. Frequencies of CD56+, MHC-I+, and S100A9+ monocytes, CCR7+ dendritic cells, CD56+dim natural killer (NK) cells and CD3+CD56bright T-cells were analyzed via flow cytometry. Serum S100A8/A9 levels were determined via ELISA. Results: SpA patients showed a reduced frequency of CD56+dim NK cells during uveitis activity, a constitutively activated monocyte phenotype, and elevated S100A8/A9 serum levels. In contrast, JIAU patients showed elevated frequencies of CD56+ monocytes and CCR7+ DC. Conclusion: Phenotype of peripheral immune cells differ between patients, probably contributing to different courses of acute onset AU in SpA and insidious onset AU in JIAU patients.Abbreviations: AU: anterior uveitis, AR: arthritis, JIA: juvenile idiopathic arthritis, SpA: axial spondyloarthritis.
Purpose: To analyze circulating immune cells in patients with anterior uveitis (AU) associated to axial spondyloarthritis (SpA), or juvenile idiopathic arthritis (JIA). Methods: Venous blood samples were collected from healthy controls (n = 16), and either SpA (n = 19) or JIA (n = 23) patients with associated anterior uveitis (AU) during active flare, or after ≥3 months of inactivity. Frequencies of CD56+, MHC-I+, and S100A9+ monocytes, CCR7+ dendritic cells, CD56+dim natural killer (NK) cells and CD3+CD56bright T-cells were analyzed via flow cytometry. Serum S100A8/A9 levels were determined via ELISA. Results: SpA patients showed a reduced frequency of CD56+dim NK cells during uveitis activity, a constitutively activated monocyte phenotype, and elevated S100A8/A9 serum levels. In contrast, JIAU patients showed elevated frequencies of CD56+ monocytes and CCR7+ DC. Conclusion: Phenotype of peripheral immune cells differ between patients, probably contributing to different courses of acute onset AU in SpA and insidious onset AU in JIAU patients.Abbreviations: AU: anterior uveitis, AR: arthritis, JIA: juvenile idiopathic arthritis, SpA: axial spondyloarthritis.
Authors: Xiwen Ji; Zicheng Zhang; Dan Lin; Mali Dai; Xia Zhao; Xingneng Guo; Jie Du; Meng Zhou; Yuqin Wang Journal: Transl Vis Sci Technol Date: 2021-11-01 Impact factor: 3.283