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Literature DB >> 3215904

Effect of chemical modification in situ on L-glycerol-3-phosphate dehydrogenase in brown adipose tissue mitochondria.

Abstract

Mitochondrial L-glycerol-3-phosphate dehydrogenase (E.C. 1.1.99.5.) was studied by chemical modification in situ with different amino acid side chain specific reagents in mitochondria isolated from hamster brown adipose tissue. The SH-modifying reagents have only slight effect on the enzyme activity. The most effective chemicals were tetranitromethane and diazobenzene sulfonic acid. The enzyme activity can be abolished completely by both of them. In the presence of Ca2+ and/or glycerol-3-phosphate inhibition was greater at the same electrophilic reagent concentration. The effect of Ca2+ and glycerol-3-phosphate is nonadditive on inhibition by these reagents.

Entities: Chemical Species

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Year: 1988 PMID： 3215904 DOI： 10.1007/bf00768923

Source DB: PubMed Journal: J Bioenerg Biomembr ISSN： 0145-479X Impact factor: 2.945

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References

24 in total

1. Reconstruction of the alpha-glycerolphosphate shuttle using rat kidney mitochondria.

Authors: A G Dawson; G J Cooney
Journal: FEBS Lett Date: 1978-07-15 Impact factor: 4.124

2. Regulatory role of reducing-equivalent transfer from substrate to oxygen in the hepatic metabolism of glycerol and sorbitol.

Authors: M N Berry; E Kun; H V Werner
Journal: Eur J Biochem Date: 1973-03-15

3. The effects of ruthenium red on reactions of blowfly flight muscle mitochondria with calcium.

Authors: E Carafoli; B Sacktor
Journal: Biochem Biophys Res Commun Date: 1972-12-18 Impact factor: 3.575

4. Ca2+ stimulation of rat liver mitochondrial glycerophosphate dehydrogenase.

Authors: M E Wernette; R S Ochs; H A Lardy
Journal: J Biol Chem Date: 1981-12-25 Impact factor: 5.157

5. Phosphate carrier of liver mitochondria: the reaction of its SH groups with mersalyl, 5,5'-dithio-bis-nitrobenzoate, and N-ethylmaleimide and the modulation of reactivity by the energy state of the mitochondria.

Authors: A Fonyo; P V Vignais
Journal: J Bioenerg Biomembr Date: 1980-08 Impact factor: 2.945

6. Influence of the activity of mitochondrial alpha-glycerophosphate oxidase on the alpha-glycerophosphate shuttle during ethanol oxidation.

Authors: A R Pösö
Journal: FEBS Lett Date: 1977-11-15 Impact factor: 4.124

Effect of chemical modification in situ on L-glycerol-3-phosphate dehydrogenase in brown adipose tissue mitochondria.

1. Reconstruction of the alpha-glycerolphosphate shuttle using rat kidney mitochondria.

2. Regulatory role of reducing-equivalent transfer from substrate to oxygen in the hepatic metabolism of glycerol and sorbitol.

3. The effects of ruthenium red on reactions of blowfly flight muscle mitochondria with calcium.

4. Ca2+ stimulation of rat liver mitochondrial glycerophosphate dehydrogenase.

5. Phosphate carrier of liver mitochondria: the reaction of its SH groups with mersalyl, 5,5'-dithio-bis-nitrobenzoate, and N-ethylmaleimide and the modulation of reactivity by the energy state of the mitochondria.

6. Influence of the activity of mitochondrial alpha-glycerophosphate oxidase on the alpha-glycerophosphate shuttle during ethanol oxidation.

7. Effect of chemical modifiers of amino acid residues on proton conduction by the H+-ATPase of mitochondria.

8. Regulation of alpha-glycerophosphate dehydrogenase activity in human term placental mitochondria.

9. Purification and properties of L-3-glycerophosphate dehydrogenase from pig brain mitochondria.

10. Purification and characterization of glycerol-3-phosphate dehydrogenase (flavin-linked) from rat liver mitochondria.