Literature DB >> 3215526

Enzymatic amplification and characterization of large DNA fragments from genomic DNA.

P Keohavong1, C C Wang, R S Cha, W G Thilly.   

Abstract

Conditions for DNA amplification in vitro using modified T7 DNA polymerase have been devised to obtain 2000-bp DNA fragments of the HGPRT gene directly from human genomic DNA. The DNA obtained from a 1.2 x 10(5)-fold amplification has been used for direct sequencing.

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Year:  1988        PMID： 3215526     DOI： 10.1016/0378-1119(88)90094-7

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  8 in total

1.  Analysis of mutational spectra by denaturing capillary electrophoresis.

Authors:  Per O Ekstrøm; Konstantin Khrapko; Xiao-Cheng Li-Sucholeiki; Ian W Hunter; William G Thilly
Journal:  Nat Protoc       Date:  2008       Impact factor: 13.491

Review 2.  The polymerase chain reaction: an improved method for the analysis of nucleic acids.

Authors:  H P Vosberg
Journal:  Hum Genet       Date:  1989-08       Impact factor: 4.132

Review 3.  Detection of single base changes in nucleic acids.

Authors:  R G Cotton
Journal:  Biochem J       Date:  1989-10-01       Impact factor: 3.857

4.  Fidelity of DNA polymerases in DNA amplification.

Authors:  P Keohavong; W G Thilly
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

5.  The use of factorial design, image analysis, and an efficiency calculation for multiplex PCR optimization.

Authors:  José Luis Villarreal Camacho; Evelyn Mendoza Torres; Christian Cadena; Julieth Prieto; Lourdes Luz Varela Prieto; Daniel Antonio Villanueva Torregroza
Journal:  J Clin Lab Anal       Date:  2013-05       Impact factor: 2.352

6.  Activation of the c-Ki-ras oncogene in aflatoxin B1-induced hepatocellular carcinoma and adenoma in the rat: detection by denaturing gradient gel electrophoresis.

Authors:  N R Soman; G N Wogan
Journal:  Proc Natl Acad Sci U S A       Date:  1993-03-01       Impact factor: 11.205

7.  Sex determination from mesiodens of Indian children by amelogenin gene.

Authors:  Mohit Srivastava; Swati Tripathi; Madhusudan Astekar; Deepa Singal; Aditi Srivastava; Pallavi Vashisth
Journal:  J Forensic Dent Sci       Date:  2017 Sep-Dec

8.  The ActA protein of Listeria monocytogenes acts as a nucleator inducing reorganization of the actin cytoskeleton.

Authors:  S Pistor; T Chakraborty; K Niebuhr; E Domann; J Wehland
Journal:  EMBO J       Date:  1994-02-15       Impact factor: 11.598
  8 in total

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