Guolin Chen1,2,3, Jingshen Zhuang1,2,3, Qianwen Cui4, Shuwen Jiang1,2,3, Weihua Tao1, Wanqun Chen4, Shuqing Yu1, Lina Wu1, Wah Yang1,2,3, Fucheng Liu5, Jingge Yang6,7,8, Cunchuan Wang9,10,11, Shiqi Jia12,13,14,15. 1. The First Affiliated Hospital, Jinan University, Huangpu Avenue West 613, Tianhe District, Guangzhou, Guangdong, China. 2. Joint Institute of Metabolic Medicine between Jinan University and the University of Hong Kong, Huangpu Avenue West 613, Tianhe District, Guangzhou, Guangdong, China. 3. Institute of Obesity and Metabolism, Jinan University, Huangpu Avenue West 613, Tianhe District, Guangzhou, Guangdong, China. 4. Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Jinan University, Guangzhou, China. 5. Department of Cardiology of the First Affiliated Hospital, Jinan University, Guangzhou, China. 6. The First Affiliated Hospital, Jinan University, Huangpu Avenue West 613, Tianhe District, Guangzhou, Guangdong, China. dukeyjg@126.com. 7. Joint Institute of Metabolic Medicine between Jinan University and the University of Hong Kong, Huangpu Avenue West 613, Tianhe District, Guangzhou, Guangdong, China. dukeyjg@126.com. 8. Institute of Obesity and Metabolism, Jinan University, Huangpu Avenue West 613, Tianhe District, Guangzhou, Guangdong, China. dukeyjg@126.com. 9. The First Affiliated Hospital, Jinan University, Huangpu Avenue West 613, Tianhe District, Guangzhou, Guangdong, China. twcc@jnu.edu.cn. 10. Joint Institute of Metabolic Medicine between Jinan University and the University of Hong Kong, Huangpu Avenue West 613, Tianhe District, Guangzhou, Guangdong, China. twcc@jnu.edu.cn. 11. Institute of Obesity and Metabolism, Jinan University, Huangpu Avenue West 613, Tianhe District, Guangzhou, Guangdong, China. twcc@jnu.edu.cn. 12. The First Affiliated Hospital, Jinan University, Huangpu Avenue West 613, Tianhe District, Guangzhou, Guangdong, China. jshiqi@aliyun.com. 13. Joint Institute of Metabolic Medicine between Jinan University and the University of Hong Kong, Huangpu Avenue West 613, Tianhe District, Guangzhou, Guangdong, China. jshiqi@aliyun.com. 14. Institute of Obesity and Metabolism, Jinan University, Huangpu Avenue West 613, Tianhe District, Guangzhou, Guangdong, China. jshiqi@aliyun.com. 15. Institute of Clinical Medicine, Jinan University, Huangpu Avenue West 613, Tianhe District, Guangzhou, Guangdong, China. jshiqi@aliyun.com.
Abstract
AIMS: To explore the intestinal microbiota composition affected by the two most widely used procedures of bariatric surgery, laparoscopic sleeve gastrectomy (LSG) and laparoscopic roux-en-Y gastric bypass (LRYGB), in Chinese obesity patients. METHODS: Stool samples were collected from the obese patients before (n = 87) and with follow-up after the surgery (n = 53). After DNA extraction, 16S rDNA (V3 + V4 regions) sequencing was completed on Illumina HiSeq 2500 sequencing platform. The samples were analyzed base on four groups, pre-LSG (n = 54), pre-LRYGB (n = 33), post-LSG (n = 33), and post-LRYGB (n = 20). The linear mixed models were used to analyze the alteration of intestinal microbiota before and after the surgeries of LSG or LRYGB. Student's t test and χ2 test were used for analysis of independent groups; Metastats analysis was used to compare the relative abundance of bacteria, and Pearson correlation and Spearman correlation analysis were used to test the correlation between indicated groups. RESULTS: 87 patients were included and 53 (60.92%) of them completed the follow-up (9.60 ± 3.92 months). Body mass index (BMI) decreased from 37.84 ± 6.16 kg/m2 to 26.22 ± 4.33 kg/m2 after LSG and from 45.75 ± 14.26 kg/m2 to 33.15 ± 10.99 kg/m2 after LRYGB. The relative abundance of 5 phyla and 42 genera were altered after the surgery in the cohort. Although no alteration of Firmicutes was observed at phylum level, 54.76% of the altered genera belong to phylum Firmicutes. Both LSG and LRYGB procedures increased the richness and evenness of intestinal microbiota in obese patients after the surgery. Particularly, 33 genera altered after LSG and 19 genera altered after LRYGB, in which 11 genera were common alterations in both procedures. CONCLUSION: Both LSG and LRYGB altered the composition of intestinal microbiota in Chinese obesity patients, and particularly increased the richness and evenness of microbiota. Genera belonging to phylum Firmicutes were the most altered bacteria by bariatric surgery. The procedure of LSG resulted in much more pronounced alteration of the intestinal microbiota abundance than that observed in LRYGB. While different genera were altered after LSG and LRYGB procedures, 10 genera were the common altered genera in both procedures. Bacteria altered after LSG and LRYGB were functionally associated with BMI, and with relieving of the metabolic syndromes.
AIMS: To explore the intestinal microbiota composition affected by the two most widely used procedures of bariatric surgery, laparoscopic sleeve gastrectomy (LSG) and laparoscopic roux-en-Y gastric bypass (LRYGB), in Chinese obesitypatients. METHODS: Stool samples were collected from the obesepatients before (n = 87) and with follow-up after the surgery (n = 53). After DNA extraction, 16S rDNA (V3 + V4 regions) sequencing was completed on Illumina HiSeq 2500 sequencing platform. The samples were analyzed base on four groups, pre-LSG (n = 54), pre-LRYGB (n = 33), post-LSG (n = 33), and post-LRYGB (n = 20). The linear mixed models were used to analyze the alteration of intestinal microbiota before and after the surgeries of LSG or LRYGB. Student's t test and χ2 test were used for analysis of independent groups; Metastats analysis was used to compare the relative abundance of bacteria, and Pearson correlation and Spearman correlation analysis were used to test the correlation between indicated groups. RESULTS: 87 patients were included and 53 (60.92%) of them completed the follow-up (9.60 ± 3.92 months). Body mass index (BMI) decreased from 37.84 ± 6.16 kg/m2 to 26.22 ± 4.33 kg/m2 after LSG and from 45.75 ± 14.26 kg/m2 to 33.15 ± 10.99 kg/m2 after LRYGB. The relative abundance of 5 phyla and 42 genera were altered after the surgery in the cohort. Although no alteration of Firmicutes was observed at phylum level, 54.76% of the altered genera belong to phylum Firmicutes. Both LSG and LRYGB procedures increased the richness and evenness of intestinal microbiota in obesepatients after the surgery. Particularly, 33 genera altered after LSG and 19 genera altered after LRYGB, in which 11 genera were common alterations in both procedures. CONCLUSION: Both LSG and LRYGB altered the composition of intestinal microbiota in Chinese obesitypatients, and particularly increased the richness and evenness of microbiota. Genera belonging to phylum Firmicutes were the most altered bacteria by bariatric surgery. The procedure of LSG resulted in much more pronounced alteration of the intestinal microbiota abundance than that observed in LRYGB. While different genera were altered after LSG and LRYGB procedures, 10 genera were the common altered genera in both procedures. Bacteria altered after LSG and LRYGB were functionally associated with BMI, and with relieving of the metabolic syndromes.
Authors: Sara Becerril; Carlota Tuero; Javier A Cienfuegos; Amaia Rodríguez; Victoria Catalán; Beatriz Ramírez; Víctor Valentí; Rafael Moncada; Xabier Unamuno; Javier Gómez-Ambrosi; Gema Frühbeck Journal: Int J Mol Sci Date: 2022-10-01 Impact factor: 6.208
Authors: Dimitrios A Koutoukidis; Susan A Jebb; Matthew Zimmerman; Afolarin Otunla; J Aaron Henry; Anne Ferrey; Ella Schofield; Jade Kinton; Paul Aveyard; Julian R Marchesi Journal: Gut Microbes Date: 2022 Jan-Dec