Literature DB >> 32151954

Generation of a male common marmoset embryonic stem cell line DSY127-BV8VT1 carrying double reporters specific for the germ cell linage using the CRISPR-Cas9 and PiggyBac transposase systems.

Sho Yoshimatsu1, Tsukika Sato2, Masafumi Yamamoto3, Erika Sasaki4, Mayutaka Nakajima2, Mari Nakamura2, Seiji Shiozawa2, Toshiaki Noce5, Hideyuki Okano6.   

Abstract

BLIMP1 (PRDM1) and VASA (DDX4) play pivotal roles in the development of the germ cell linage. Importantly, these genes are specifically expressed in germ cells; BLIMP1 in primordial germ cells (PGCs) to early-stage gonocytes, and VASA in migration-stage PGCs to mature gametes. The high reproductive efficiency of common marmosets (marmosets; Callithrix jacchus) makes them advantageous for use in germ cell research. We herein report the generation of a male marmoset embryonic stem cell (ESC) line harboring BLIMP1 and DDX4 double reporters. This ESC line will be a useful tool for investigating male gametogenesis in non-human primates.
Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

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Year:  2020        PMID： 32151954     DOI： 10.1016/j.scr.2020.101740

Source DB:  PubMed          Journal:  Stem Cell Res        ISSN： 1873-5061            Impact factor:   2.020


  4 in total

Review 1.  Utility of Common Marmoset (Callithrix jacchus) Embryonic Stem Cells in Liver Disease Modeling, Tissue Engineering and Drug Metabolism.

Authors:  Rajagopal N Aravalli; Clifford J Steer
Journal:  Genes (Basel)       Date:  2020-06-30       Impact factor: 4.096

2.  Homologous Recombination-Enhancing Factors Identified by Comparative Transcriptomic Analyses of Pluripotent Stem Cell of Human and Common Marmoset.

Authors:  Sho Yoshimatsu; Mayutaka Nakajima; Emi Qian; Tsukasa Sanosaka; Tsukika Sato; Hideyuki Okano
Journal:  Cells       Date:  2022-01-21       Impact factor: 6.600

3.  Overexpression of bmp4, dazl, nanos3 and sycp2 in Hu Sheep Leydig Cells Using CRISPR/dcas9 System Promoted Male Germ Cell Related Gene Expression.

Authors:  Hua Yang; Mingtian Deng; Wenli Lv; Zongyou Wei; Yu Cai; Peiyong Cheng; Feng Wang; Yanli Zhang
Journal:  Biology (Basel)       Date:  2022-02-11

4.  Non-viral Induction of Transgene-free iPSCs from Somatic Fibroblasts of Multiple Mammalian Species.

Authors:  Sho Yoshimatsu; Mayutaka Nakajima; Aozora Iguchi; Tsukasa Sanosaka; Tsukika Sato; Mari Nakamura; Ryusuke Nakajima; Eri Arai; Mitsuru Ishikawa; Kent Imaizumi; Hirotaka Watanabe; Junko Okahara; Toshiaki Noce; Yuta Takeda; Erika Sasaki; Rüdiger Behr; Kazuya Edamura; Seiji Shiozawa; Hideyuki Okano
Journal:  Stem Cell Reports       Date:  2021-04-01       Impact factor: 7.765
  4 in total

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