Lian Wang1, Zongbao Wang2, Qiqi Liu3, Jingchao Su4, Tianming Wang4, Tao Li5. 1. School of Kinesiology, Shanghai University of Sport, Shanghai, 200438, China. 2. Ministry of Science and Education, Anhui Provincial Hospital of Integrated Chinese and Western Medicine (The Third Affiliated Hospital of Anhui University of Chinese Medicine), No. 45, Shihe Road, Wulidun Subdistrict, Shushan District, Hefei, 230061, Anhui Province, China. wang673099692@hotmail.com. 3. Graduate School, Anhui University of Chinese Medicine, Hefei, 230038, Anhui Province, China. 4. Clinical College of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, 230038, Anhui Province, China. 5. Ministry of Science and Education, Anhui Provincial Hospital of Integrated Chinese and Western Medicine (The Third Affiliated Hospital of Anhui University of Chinese Medicine), No. 45, Shihe Road, Wulidun Subdistrict, Shushan District, Hefei, 230061, Anhui Province, China.
Abstract
INTRODUCTION: To investigate the effect of different frequencies of whole body vibration (WBV) on articular cartilage of early knee osteoarthritis (OA) rats and determine whether WBV would influence the pathway of hypoxia-inducible factor-2α (HIF-2α) regulation-related genes after 8 weeks of treatment. MATERIALS AND METHODS: Forty 8-week-old OA rats were divided into five groups: sham control (SC); high frequency 60 Hz (HV1); high frequency 40 Hz (HV2); middle frequency 20 Hz (MV) and low frequency 10 Hz (LV). WBV (0.3 g) treatment was given 40 min/day and 5 days/week. After 8 weeks, rats were killed and knees were harvested. OA grading score: Osteoarthritis Research Society International (OARSI), and the expression of related genes: interleukin-1β (IL-1β), HIF-2α, matrix metalloproteinases-13 (MMP-13), and collagen type II alpha 1 (COL2A1), at both mRNA and protein levels were analyzed. RESULTS: After 8 weeks of WBV, our data showed that lower frequency (10 Hz) was more effective than the higher ones, yet they all suggested that WBV alleviates the erosion of knee articular cartilage in early OA. The expression of IL-1β, HIF-2α and MMP-13 decreased with frequency and reached the lowest level at 10 Hz, the expression of COL2A1 increased with frequency and reached the highest level at 10 Hz. CONCLUSIONS: This study demonstrates that WBV could alleviate the degeneration of knee joints in an early OA rat model. WBV regulates related gene expression at both mRNA and protein levels. HIF-2α could be a therapeutic target. The effect of WBV is frequency dependent; the lower frequency shows better effects.
INTRODUCTION: To investigate the effect of different frequencies of whole body vibration (WBV) on articular cartilage of early knee osteoarthritis (OA) rats and determine whether WBV would influence the pathway of hypoxia-inducible factor-2α (HIF-2α) regulation-related genes after 8 weeks of treatment. MATERIALS AND METHODS: Forty 8-week-old OA rats were divided into five groups: sham control (SC); high frequency 60 Hz (HV1); high frequency 40 Hz (HV2); middle frequency 20 Hz (MV) and low frequency 10 Hz (LV). WBV (0.3 g) treatment was given 40 min/day and 5 days/week. After 8 weeks, rats were killed and knees were harvested. OA grading score: Osteoarthritis Research Society International (OARSI), and the expression of related genes: interleukin-1β (IL-1β), HIF-2α, matrix metalloproteinases-13 (MMP-13), and collagen type II alpha 1 (COL2A1), at both mRNA and protein levels were analyzed. RESULTS: After 8 weeks of WBV, our data showed that lower frequency (10 Hz) was more effective than the higher ones, yet they all suggested that WBV alleviates the erosion of knee articular cartilage in early OA. The expression of IL-1β, HIF-2α and MMP-13 decreased with frequency and reached the lowest level at 10 Hz, the expression of COL2A1 increased with frequency and reached the highest level at 10 Hz. CONCLUSIONS: This study demonstrates that WBV could alleviate the degeneration of knee joints in an early OA rat model. WBV regulates related gene expression at both mRNA and protein levels. HIF-2α could be a therapeutic target. The effect of WBV is frequency dependent; the lower frequency shows better effects.
Authors: Borja del Pozo-Cruz; Jose C Adsuar; Jose A Parraca; Jesús del Pozo-Cruz; Pedro R Olivares; Narcis Gusi Journal: J Altern Complement Med Date: 2012-01-10 Impact factor: 2.579
Authors: Nima Befrui; Jens Elsner; Achim Flesser; Jacqueline Huvanandana; Oussama Jarrousse; Tuan Nam Le; Marcus Müller; Walther H W Schulze; Stefan Taing; Simon Weidert Journal: Med Biol Eng Comput Date: 2018-02-01 Impact factor: 2.602