Modest Vengust1, Mason C Jager2, Valentina Zalig1,3, Vasilij Cociancich1, Melissa Laverack4, Randall W Renshaw4, Edward Dubovi4, Joy E Tomlinson2, Gerlinde R Van de Walle2, Thomas J Divers5. 1. University of Ljubljana, Veterinary Faculty, Ljubljana, Slovenia. 2. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA. 3. Marc Veterinary Services, Sezana, Slovenia. 4. Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA. 5. Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA.
Abstract
BACKGROUND: Equine parvovirus-hepatitis (EqPV-H) has been proposed as the aetiological cause of Theiler's disease, also known as serum hepatitis. EqPV-H-associated Theiler's disease has not been previously reported in Europe. OBJECTIVES: To determine whether EqPV-H infection was associated with a 2018-2019 outbreak of Theiler's disease in four horses on a studfarm. STUDY DESIGN: Descriptive case series. METHODS: The medical records of four horses from the same farm diagnosed with fatal Theiler's disease were examined retrospectively. Information collected included a clinical history, physical examination findings, tetanus antitoxin exposure, serum biochemistry and necropsy reports. Liver tissue from all four horses was tested for EqPV-H using PCR and in situ hybridisation (ISH) assays. RESULTS: Three of the horses had a history of recent (7-11 weeks) tetanus antitoxin administration. Liver tissue from all four horses tested positive for EqPV-H with PCR. In situ hybridisation revealed a widespread distribution of viral nucleic acid in hepatocytes in one case, and a more sporadic distribution in the remaining three cases. MAIN LIMITATIONS: Case controls were not available from the farm in question given the retrospective nature of analysis. CONCLUSIONS: This case series documents the first reported EqPV-H-associated Theiler's disease in Europe and the first use of ISH to visualise the viral nucleic acid in liver tissues of horses with Theiler's disease.
BACKGROUND: Equine parvovirus-hepatitis (EqPV-H) has been proposed as the aetiological cause of Theiler's disease, also known as serum hepatitis. EqPV-H-associated Theiler's disease has not been previously reported in Europe. OBJECTIVES: To determine whether EqPV-H infection was associated with a 2018-2019 outbreak of Theiler's disease in four horses on a studfarm. STUDY DESIGN: Descriptive case series. METHODS: The medical records of four horses from the same farm diagnosed with fatal Theiler's disease were examined retrospectively. Information collected included a clinical history, physical examination findings, tetanus antitoxin exposure, serum biochemistry and necropsy reports. Liver tissue from all four horses was tested for EqPV-H using PCR and in situ hybridisation (ISH) assays. RESULTS: Three of the horses had a history of recent (7-11 weeks) tetanus antitoxin administration. Liver tissue from all four horses tested positive for EqPV-H with PCR. In situ hybridisation revealed a widespread distribution of viral nucleic acid in hepatocytes in one case, and a more sporadic distribution in the remaining three cases. MAIN LIMITATIONS: Case controls were not available from the farm in question given the retrospective nature of analysis. CONCLUSIONS: This case series documents the first reported EqPV-H-associated Theiler's disease in Europe and the first use of ISH to visualise the viral nucleic acid in liver tissues of horses with Theiler's disease.
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