Yafei Li1,2, Qianyue Jin1,3, Peiyang Ding4, Wen Zhou1,2, Yongxiao Chai1,2, Xufeng Li1,5, Yao Wang1,5, Gaiping Zhang6,7,8,9,10. 1. Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China. 2. College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, China. 3. Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China. 4. School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, China. 5. College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, 450002, China. 6. Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China. zhanggaip@126.com. 7. College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, China. zhanggaip@126.com. 8. College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, 450002, China. zhanggaip@126.com. 9. School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, China. zhanggaip@126.com. 10. Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China. zhanggaip@126.com.
Abstract
OBJECTIVE: To create a novel subunit vaccine that used AuNPs as carriers to enhance immune responses in mice against recombinant classical swine fever virus E2 protein (CSFV E2). RESULTS: Gold nanoparticles (AuNPs) were successfully coupled to the E2 protein and formed stable particle complexes called E2 conjugated AuNPs (E2-AuNPs). In vitro studies have shown that the E2-AuNPs complex has the same immunogenicity as the E2 protein, and AuNPs can promote the phagocytosis of the E2 protein by antigen-presenting cells (APCs). In vivo results of BALB/c mice showed that the antibody levels, lymphocyte proliferation index, IFN-γ and IL-10 cytokines induced by E2-AuNPs were relatively higher than those of E2 or AuNPs group. CONCLUSIONS: This finding demonstrated the potential of using AuNPs as a carrier to enhance the body's immune response for developing CSFV subunit vaccines. This model also contributes to the development of other flavivirus subunit vaccines, such as hepatitis C virus (HCV) and bovine viral diarrhea virus (BVDV).
OBJECTIVE: To create a novel subunit vaccine that used AuNPs as carriers to enhance immune responses in mice against recombinant classical swine fever virusE2 protein (CSFV E2). RESULTS: Gold nanoparticles (AuNPs) were successfully coupled to the E2 protein and formed stable particle complexes called E2 conjugated AuNPs (E2-AuNPs). In vitro studies have shown that the E2-AuNPs complex has the same immunogenicity as the E2 protein, and AuNPs can promote the phagocytosis of the E2 protein by antigen-presenting cells (APCs). In vivo results of BALB/c mice showed that the antibody levels, lymphocyte proliferation index, IFN-γ and IL-10 cytokines induced by E2-AuNPs were relatively higher than those of E2 or AuNPs group. CONCLUSIONS: This finding demonstrated the potential of using AuNPs as a carrier to enhance the body's immune response for developing CSFV subunit vaccines. This model also contributes to the development of other flavivirus subunit vaccines, such as hepatitis C virus (HCV) and bovine viral diarrhea virus (BVDV).