Synthesis and biological evaluation of a series of 2-(((5-akly/aryl-1H-pyrazol-3-yl)methyl)thio)-5-alkyl-6-(cyclohexylmethyl)-pyrimidin-4(3H)-ones as potential HIV-1 inhibitors.

A series of 2-(((5-akly/aryl-1H-pyrazol-3-yl)methyl)thio)-5-alkyl-6-(cyclohexylmethyl)-pyrimidin-4(3H)-ones were synthesized and their anti-HIV-1 activities were evaluated. Most of these compounds were highly active against wild-type (WT) HIV-1 strain (IIIB) with EC50 values in the range of 0.0038-0.4759 μmol/L. Among those compounds, I-11 had an EC50 value of 3.8 nmol/L and SI (selectivity index) of up to 25,468 indicating excellent activity against WT HIV-1. In vitro anti-HIV-1 activity and resistance profile studies suggested that compounds I-11 and I-12 displayed potential anti-HIV-1 activity against laboratory adapted strains and primary isolated strains including different subtypes and tropism strains (EC50s range from 4.3 to 63.6 nmol/L and 18.9-219.3 nmol/L, respectively). On the other hand, it was observed that those two compounds were less effective with EC50 values of 2.77 and 4.87 μmol/L for HIV-1A17 (K103N + Y181C). The activity against reverse transcriptase (RT) was also evaluated for those compounds. Both I-11 and I-12 obtained sub-micromolar IC50 values showing their potential in RT inhibition. The pharmacokinetics examination in rats indicated that compound I-11 has acceptable pharmacokinetic properties and bioavailability. Preliminary structure-activity relationships and molecular modeling studies were also discussed.

Introduction

Noical">n-nucleoside reverse transcriptase inhibitors (NNRTIs) are indispensable component of highly active antiretroviral therapy (HAART) that is widely used in the clinical treatment of HIV-1-infected patients1, 2, 3. Currently, five NNRTIs have been approved for clinical use: nevirapine (NVP), delavirdine (DLV), efavirenz (EFV), etravirine (ETR, TMC125), and rilpivirine (RPV, TMC278),. However, therapeutic effectiveness of these available drugs has been limited to a certain extent by the emergence of drug-resistant viruses and potentially severe side effects with long-term clinical use6, 7, 8. As a result, discovery of novel NNRTIs candidates with better resistance profiles and improved safety and tolerability is a continuous goal of drug development9, 10, 11, 12, 13.

Among NNRTIs of clinical interest, 2-alkylsulfanyl-6-benzyl-3,4-dihydropyrimidin-4(3H)-ones (S-DABOs) occupy a significant place for their unique antiviral potency, high specificity and low toxicity14, 15, 16. Studies on S-DABOs suggested that an alkyl/arylthio substituent at the C2 position, an aromatic ring linked through a methlyene bridge to the C6 position and the unmodified NHCO fragment constituting the N3 and C4 positions of the prymidine ring are structural determinants for the antiviral activity of these compounds17, 18, 19, 20. The key interactions of S-DABOs with HIV-1 reverse transcriptase (RT) residues could be summarized as Fig. 1.

Figure 1

Structures of S-DABOs and newly designed compounds.

Previous research efforts in our lab led to the identification of a series of oxophenethyl-S-DABOs derivatives with significant anti-HIV-1 activity21, 22, 23, 24. Especially, by replacing the C6-arylring with a C6-cyclohexylmethyl moiety, a new series oxophenethyl-S-DACOs of NNRTIs with higher potent and specificity was obtained. The most promising compound DB02 exhibited potent anti-HIV-1 activity against laboratory adapted strains and primary isolated strains (EC50s (concentrations inhibiting virus replication by 50%) range from 2.40 to 41.8 nmol/L), along with an improved sensitivity against K103N or Y181C than S-DABOs26. The molecular modeling study (Fig. 1) indicated that the carbonyl oxygen of C2 side chain of DB02 forms a key H-bond with the backbone (–NH– group) of K103 of HIV-1 RT which acts as essential pharmacophore elements favorably contributing to ligand binding,. Since the ω-phenyl of C2 side chain point at the interface between the enzyme and the solvent, we could conclude that longer and diverse groups are tolerant of this region. The proposed binding mode for DB02 allowed us to anticipate that the introduction of a pyrazol group, which contains both hydrogen bond donor and acceptor, at C2 side chain instead of a carbonyl group might further strengthen the hydrogen interactions with K103 of the viral enzyme RT. This could result in an improved potency against the wild-type or mutant HIV-1 strains. Meanwhile, to develop π–π stack and van der Waals' interactions with the amino residues in this hydrophobic region around the C2 side chain, different substituents varying in their size and electronic nature were introduced to the C5′ and C3′ position of the pyrazol ring. Thus, a series of novel 2-(((5-akly/aryl-1H-pyrazol-3-yl)me-thyl)thio)-5-alkyl-6-(cyclohexylmethyl)-pyrimidin-4(3H)-one derivatives were designed based on the analysis of the ligand–receptor interactions (Fig. 1). In the newly designed analogs, different substituents varying in size and electronic nature were introduced to the ω-phenyl of C2 side chain located at the protein-solvent interface region of RT to further investigate the potential interaction. In this paper, we report the synthesis of this novel S-DACOs, the evaluation of their anti-HIV-1 activity and the structure–activity relationship (SAR) study in detail.

Results and discussion

Chemistry

The target compounds I (1–40) were prepared in a convergent manner by coupling two subunits as depicted in Scheme 1. Following the procedure described previously,, the key intermediate β-ketoesters 3 were prepared by exposure of 2-cyclohexylacetic acid 1 to 1,1′-carboical">nyl-diimidazole (n class="Chemical">CDI) followed by treatment with different ethyl potassium malonates in the presence of anhydrous MgCl2 and Et3N. Condensation of 3 with thiourea in the presence of EtONa in refluxing EtOH led to substituted uracil 4 which was subjected to S-alkylation in anhydrous DMF with 3-(bromomethyl)-5-R1-1H-pyrazole (10) in the presence of K2CO3 to afford the target compounds I (1–40). 10 required for the S-alkylation was prepared from appropriate methyl ketones 5. The addition of methyl ketones 5 to diethyl malonate, followed by the cyclization with hydrazine for the pyrazole ring formation under refluxing condition gave the desired 5-substituted-1H-pyrazole-3-carboxylate (7) over two steps,. Halogenation on the pyrazole with NCS produced chlorinated pyrazole 8 in good yield. The ester moiety was then converted into the alcohol 9 by reduction with LiAlH4 and then bromination with PBr3 or Br2 reagent. These synthesized compounds were characterized by physicochemical and spectral means. MS, 1H NMR, 13C NMR spectral data were found in agreement with the assigned molecular structures.

Scheme 1

Synthesis of compounds I (1−40). Reagents and conditions: (i) diethyl malonate, EtONa, rt, 14 h; (ii) hydrazine hydrate, EtOH, reflux, 3 h; (iii) NCS, CH3CN, DMF, 55 °C, 15 h; (iv) LiAlH4, THF, 0 °C, 3 h; (v) PBr3, or (Ph)3P, Br2 CH3CN, reflux, or rt, 2 h; (vi) (a) MgCl2, Et3N, CH3CN, rt, 2 h; (b) CDI, rt, overnight then reflux, 2 h; (vii) thiourea, EtONa, EtOH, reflux, 6 h; (viii) DMF, K2CO3, rt, 3–12 h.

Synthesis of compounds I (1−40). Reagents and coical">nditions: (i) n class="Chemical">diethyl malonate, EtONa, rt, 14 h; (ii) hydrazine hydrate, EtOH, reflux, 3 h; (iii) NCS, CH3CN, DMF, 55 °C, 15 h; (iv) LiAlH4, THF, 0 °C, 3 h; (v) PBr3, or (Ph)3P, Br2 CH3CN, reflux, or rt, 2 h; (vi) (a) MgCl2, Et3N, CH3CN, rt, 2 h; (b) CDI, rt, overnight then reflux, 2 h; (vii) thiourea, EtONa, EtOH, reflux, 6 h; (viii) DMF, K2CO3, rt, 3–12 h.

Anti-HIV-1 activity evaluation

The novel S-DACO derivatives I (1–40) were tested for their n class="Disease">cytotoxicity and anti-HIV-1 activity in C8166 cells infected by the HIV-1IIIB and compared with nevirapine (NVP), etravirine (ETR) and zidovudine (AZT). The activity data was interpreted in CC50 (concentration resulting in 50% cell death) values (cytotoxicity), EC50 (anti-HIV-1 activity) and SI (selectivity index, given by the CC50/EC50 ratio) (Table 1). As another control, previously reported compound DB02, was included in our test assays for comparison.

Table 1

Activity against HIV-1IIIB strain, cytotoxicity and SI of the title compounds in C8166 cellsa.

Compd.	R1	R2	X	EC50b (μmol/L)	CC50c (μmol/L)	SId
I-01	Ph	Et	H	0.0068 ± 0.0017	84.48 ± 0.91	12,424
I-02	3′-Me-Ph	Et	H	0.0739 ± 0.0079	82.76 ± 2.20	1120
I-03	3′-Br-Ph	Et	H	0.0512 ± 0.0073	76.26 ± 3.09	1489
I-04	3′-Cl-Ph	Et	H	0.0665 ± 0.0388	80.99 ± 0.38	1218
I-05	3′-F-Ph	Et	H	0.0348 ± 0.0206	71.99 ± 8.86	2069
I-06	3′-CF3-Ph	Et	H	0.3016 ± 0.2135	34.42 ± 3.50	114
I-07	4′-Me-Ph	Et	H	0.0523 ± 0.0385	91.42 ± 1.90	1748
I-08	4′-Cl-Ph	Et	H	0.0178 ± 0.0112	83.86 ± 0.45	4711
I-09	4′-F-Ph	Et	H	0.0200 ± 0.0018	74.93 ± 4.82	3747
I-10	4′-MeO-Ph	Et	H	0.0232 ± 0.0071	>200	>8621
I-11	4′-OH-Ph	Et	H	0.0038 ± 0.0011	96.78 ± 8.17	25,468
I-12	4′-MeS-Ph	Et	H	0.0118 ± 0.0088	>200	>16,949
I-13	4′-(CH3)2CH-Ph	Et	H	0.1469 ± 0.0552	31.12 ± 3.81	212
I-14	3′,4′-diCl-Ph	Et	H	0.3102 ± 0.2008	75.35 ± 8.48	243
I-15	3′,4′-diF-Ph	Et	H	0.1617 ± 0.0371	86.43 ± 3.10	535
I-16	2′,4′-diMe-Ph	Et	H	0.4759 ± 0.4129	87.39 ± 1.87	184
I-17	2′,4′-diF-Ph	Et	H	0.0418 ± 0.0031	59.71 ± 8.78	1428
I-18	2′-N-pyridyl	Et	H	0.0228 ± 0.0017	79.44 ± 5.18	3484
I-19	H	Et	H	0.0334 ± 0.0056	75.01 ± 6.88	2246
I-20	Me	Et	H	0.0277 ± 0.0015	45.16 ± 10.71	1630
I-21	Cyclopropyl	Et	H	0.0245 ± 0.0027	47.19 ± 4.89	1926
I-22	C(CH3)3	Et	H	0.2965 ± 0.1034	67.93 ± 4.76	229
I-23	Ph	Et	Cl	0.0990 ± 0.0019	>200	>2020
I-24	3′-Me-Ph	Et	Cl	0.0860 ± 0.0152	77.10 ± 13.61	897
I-25	3′-Br-Ph	Et	Cl	0.1015 ± 0.0025	58.06 ± 9.84	572
I-26	3′-Cl-Ph	Et	Cl	0.0570 ± 0.0033	66.08 ± 31.43	1159
I-27	3′-F-Ph	Et	Cl	0.0713 ± 0.0436	>200	>2805
I-28	3′-CF3-Ph	Et	Cl	0.1819 ± 0.0116	55.05 ± 3.82	303
I-29	4′-Me-Ph	Et	Cl	0.1380 ± 0.0469	>200	>1449
I-30	4′-Cl-Ph	Et	Cl	0.0893 ± 0.0629	>200	>2240
I-31	4′-F-Ph	Et	Cl	0.0181 ± 0.0017	64.92 ± 14.55	3587
I-32	3′,4′-diCl-Ph	Et	Cl	0.3495 ± 0.0197	190.20 ± 13.85	544
I-33	3′,4′-diF-Ph	Et	Cl	0.1512 ± 0.0960	115.43 ± 19.05	763
I-34	4′-MeO-3′-Cl-Ph	Et	Cl	0.1299 ± 0.0508	49.84 ± 8.21	384
I-35	Ph	CH3	H	0.1865 ± 0.0936	54.62 ± 2.88	293
I-36	4′-MeO-Ph	CH3	H	0.1941 ± 0.0103	66.88 ± 0.86	345
I-37	4′-F-Ph	CH3	H	0.3552 ± 0.1654	42.23 ± 1.75	119
I-38	Ph	H	H	4.9825 ± 2.5257	79.06 ± 5.34	16
I-39	4′-MeO-Ph	H	H	3.9567 ± 2.0214	56.05 ± 0.89	14
I-40	4′-F-Ph	H	H	10.0751 ± 4.1284	61.62 ± 3.22	6
DB02	–	–	–	0.0067 ± 0.0021	>200	>29,851
ETR	–	–	–	0.0014 ± 0.0034	27.48 ± 5.69	19,628
NVP	–	–	–	0.0402 ± 0.0257	>200	>4975
AZT	–	–	–	0.0089 ± 0.0002	>200	>22,472

–Not applicable.

All data represent as mean ± SD (n = 3).

Effective concentration required to protect C8166 cell against the cytopathogenicity of HIV by 50%.

Cytostatic concentration required to reduce C8166 cell proliferation by 50% tested by MTT method.

Selectivity index: ratio CC50/EC50, a higher SI means a more selective compound.

Activity against HIV-1IIIB strain, n class="Disease">cytotoxicity and SI of the title compounds in C8166 cellsa.

As shown in Table 1, most of the target molecules exhibited good to excellent anti-HIV-1 (WT, wild-type) activity with EC50 raical">ngiical">ng from 0.0038 to 0.4759 μmol/L except for compounds n class="Chemical">I-38, I-39 and I-40 with EC50s of 4.9825, 3.9567 and 10.0751 μmol/L respectively. Other 12 compounds (I-01, I-05, I-08, I-09, I-10, I-11, I-12, I-18, I-20, I-21, I-22 and I-31) were found more potent than the reference drug NVP (EC50 = 0.0402 μmol/L) against HIV-1. The most potent compounds were I-01 and I-11 with EC50 values of 0.0068 and 0.0038 μmol/L, respectively. They were about 10 times more potent than NVP and were comparable with that observed with DB02 (EC50 = 0.0067 μmol/L) and AZT (EC50 = 0.0089 μmol/L), but slightly lower than ETR (EC50 = 0.0014 μmol/L). Most compounds showed low to moderate cytotoxicity, and six compounds (I-10, I-12, I-23, I-27, I-29 and I-30) out of them had CC50 > 200 μmol/L.

Based on above anti-HIV-1 results, prelimiical">nary SARs of these compounds could be deliical">neated as the followiical">ng. The side chain connected to C2 position of the pyrimidine ring is a major determinant for the anti-HIV-1 activity of DABOs. Obviously the (1H-pyrazol-3-yl)methyl-thio is favorable to the anti-HIV-1 activities. Compound I-19 displayed an EC50 value of 0.0334 μmol/L which is similar to that of the reference drug NVP (0.0402 μmol/L). The diverse groups connecting to the C5′ position of the pyrazole ring are also studied. Changing from hydrogen (I-19) to Me and cyclopropyl gave compounds I-20 and I-21 similar EC50 value of 0.0277 and 0.0245 μmol/L respectively. However, replacing of the C5′ hydrogen of I-19 with a tert-butyl decreased the activity for more than 10 folds (I-22 vs. I-19). Substituting aryl at the C5′ position of the pyrazole ring could increase the activity against WT HIV-1 as those compounds which bearing phenyl group at the R1 position displayed the most potent anti-HIV activity.

Furthermore, the SAR analysis of substitutions oical">n the ω-phenyl of C2 side chain was explored. Compared with the non-substituted compound I-01, introduction of substituent to the phenyl ring seemed to be unfavorable to the HIV-1 inhibitory activities demonstrated by I-02–I-34 activities. However, the exceptions were 4′-OH (I-11, EC50 = 0.0038 μmol/L, SI = 25,468) and 4′-SMe (I-12, EC50 = 0.0118 μmol/L, SI = 16,949) which enhanced the activities significantly and along with their low cytotoxicity, resulted in extremely high SI. In addition, the monosubstituted phenyl analogs were more potent than disubstituted derivatives (I-2 and I-13 vs. I-14–I-17). For monosubstituted compounds, the activity of para-substituted derivatives was slightly superior to that of meta-substituted derivatives, for example, I-07>I-02, I-08>I-04, I-09>I-05. Meanwhile, the nature of the substituent of the phenyl ring also influenced the antiviral activity of these novel S-DACOs, a clear order of C4′-substitution for anti-HIV activity was observed by direct comparison: OH (I-11, SI = 25,468)>SCH3 (I-12, SI > 16,949)>H (I-01, SI = 12,424)>OCH3 (I-10, SI > 8621)>Cl (I-08, SI = 4711)>F (I-09, SI = 3747)>Me (I-07, SI = 1748)>iPr (I-13, SI = 212).

Since the iical">ntroductioical">n of a n class="Chemical">halogen atom to bioactive molecules could have significant influence on pharmaceutical profiles, the chlorine atom was also introduced to 4′-position (X substitution) of pyrazol ring to observe the effects on anti-HIV-1 activity. The results demonstrated that for some compounds, the inhibitory activities (EC50 values) against WT HIV-1 were marginally affected by the substitutions (H or Cl) at the 4′ position of the pyrazol nucleus. Through the pairwise comparison of the EC50 values, namely I-02 (X = H, EC50 = 0.0739 μmol/L) and I-24 (X = Cl, EC50 = 0.0860 μmol/L); I-04 (X = H, EC50 = 0.0665 μmol/L) and I-26 (X = Cl, EC50 = 0.0570 μmol/L); I-06 (X = H, EC50 = 0.3016 μmol/L) and I-28 (X = Cl, EC50 = 0.1819 μmol/L); I-09 (X = H, EC50 = 0.0200 μmol/L) and I-31 (X = Cl, EC50 = 0.0181 μmol/L); I-14 (X = H, EC50 = 0.3102 μmol/L) and I-32 (X = Cl, EC50 = 0.3495 μmol/L); I-15 (X = H, EC50 = 0.1617 μmol/L) and I-33 (X = Cl, EC50 = 0.1512 μmol/L), some compound's incorporation of a chlorine to C4′ position of pyrazol decreased the activity for more than 2–15 folds such as I-23 vs. I-01 (decreased 15 folds), I-30 vs. I-08 (decreased 5 folds), I-27 vs. I-05 and I-29 vs. I-07 (decreased 2 folds). Moreover, regarding the cytotoxicity of these compounds, some 4′-Cl substituted compounds (I-23, I-30, I-27 and I-29) were much less toxic with the CC50 values higher than 200 μmol/L while the unsubstituted counterparts (I-01, I-08, I-05 and I-07) had CC50 values ranging from 83 to 85 μmol/L. On the other hand, some unsubstituted compounds (I-02, I-03, I-04 and I-09) have slightly higher CC50 values than their 4′-Cl substituted counterparts (I-24, I-25, I-26 and I-31). Overall, average cytotoxicity of the 4′-Cl substituted compounds are lower than that of the unsubstituted compounds.

The previous SAR for S-DABOs iical">ndicated that the optimal moieties at positions 5 of the pyrimidine nucleus were dependent on the nature of the C2 and C6 side chain,,31, 32, 33. This series clearly showed that inhibitory activity increased proportionally with the modification of the C5 substituent in the order, H < Me < Et. As demonstrated, the 5-Et substituted derivatives (I-10, I-09 and I-01) were 8–27-fold (SI ratio) more active than their 5-Me substituted counterparts (I-36, I-37 and I-35), and 170–731-folds more active than their 5-H substituted counterparts (I-39, I-40 and I-38). This confirmed that the steric bulkiness of C5 substituent is favored to maintain inhibitory activity against HIV-1 replication.

In vitro anti-HIV-1 activity of I-11 and I-12

Based on the results of the first round of screening, we performed further antiviral activity evaluation of I-11 and I-12 as representative compounds aical">nd assessed the activity against different n class="Species">HIV-1 strains including wild-type strains (HIV-1IIIB and HIV-1Ba-L), resistant strains (HIV-1A17, HIV-174V and HIV-1RF/V82F/184V) and clinical isolated strains (HIV-1TC-1 and HIV-1WAN). DB02 and NVP were regarded as positive controls. The EC50, CC50 and SI data are summarized in Table 2. As shown in the Table 2, I-11 exhibited high potency antiviral replication against most test HIV-1 strains in different cells. In particular, the EC50 values of I-11 against HIV-1IIIB, HIV-1Ba-L and HIV-1TC-1, the major isolates from high AIDS prevalence region in China were 0.0043, 0.0485 and 0.0145 μmol/L, respectively. These were about 5–14 times more potent than NVP (EC50 = 0.0274, 0.1659 and 0.1992 μmol/L, respectively) and were comparable to that observed with DB02 (EC50 = 0.0095, 0.0377 and 0.0151 μmol/L, respectively). The antiviral effect of I-12 is weaker and EC50 values ranging from 0.0189 to 0.2193 nmol/L. However, for HIV-1A17, a multi-drug resistant strain of NNRTIs carrying the K103N and Y181C mutations, I-11, I-12 and DB02 all showed a significantly reduced inhibitory activity with EC50 values of 2.77, 4.87 and 6.01 μmol/L. Since the new generation of NNRTI, etravirine, showed a better inhibitory activity against K103N/Y181C double mutant HIV-1 strain (EC50=0.050 μmol/L), more improvements will be required to our compounds.

Table 2

Anti-HIV-1 activities of I-11 and I-12 against wild-type strains, clinical isolated strains, and resistant strainsa.

Strain	Cell	EC50 (μmol/L)				CC50 (μmol/L)				SI
		I-11	I-12	DB02	NVP	I-11	I-12	DB02	NVP	I-11	I-12	DB02	NVP
HIV-1IIIB	C8166	0.0043 ± 0.0023	0.0189 ± 0.0058	0.0095 ± 0.0017	0.0274 ± 0.0068	94.48 ± 2.40	>200	>200	>200	21,514	>10,582	>21,053	>7299
HIV-1Ba-L	TZM-b1	0.0485 ± 0.0068	0.1933 ± 0.0261	0.0377 ± 0.0231	0.1659 ± 0.0465	77.10 ± 3.68	>200	>200	>200	1590	>1035	>5305	>1206
HIV-1TC-1	PBMC	0.0145 ± 0.0001	0.0554 ± 0.0112	0.0118 ± 0.0006	0.1992 ± 0.0487	46.84 ± 5.20	>200	>200	>200	3230	>3610	>16,949	>1004
HIV-1WAN	PBMC	0.0566 ± 0.0024	0.1651 ± 0.620	0.0151 ± 0.0025	0.0770 ± 0.0193	46.84 ± 5.20	>200	>200	>200	828	>1211	>13,245	>2597
HIV-174Vb	C8166	0.0462 ± 0.0237	0.0881 ± 0.0179	0.0249 ± 0.0099	0.0768 ± 0.0193	94.48 ± 2.40	>200	>200	>200	2045	>2270	>8032	>2604
HIV-1RF/V82F/184Vc	C8166	0.0541 ± 0.0231	0.2193 ± 0.0465	0.0465 ± 0.0089	0.0614 ± 0.0085	94.48 ± 2.40	>200	>200	>200	951	>912	>4301	>3257
HIV-1A17d	C8166	2.77 ± 0.82	4.87 ± 2.71	6.01 ± 1.05	20.15 ± 3.10	94.48 ± 2.40	>200	>200	>200	34	>41	>33	>10

Mean activity of EC50 was exhibited by mean ± SD, n ≥ 3.

Mutation site of HIV-1RF/V82F/184V are V82F and M184V in protease encoding region in pol gene.

Mutation site of HIV-174V is L74V in reverse transcriptase (RT) encoding region in pol gene.

Mutation sites of HIV-1A17 are K103N and Y181C in RT encoding region in pol gene.

Anti-HIV-1 activities of I-11 and n class="Chemical">I-12 against wild-type strains, clinical isolated strains, and resistant strainsa.

Mutation site of n class="Species">HIV-174V is L74V in reverse transcriptase (RT) encoding region in pol gene.

Mutation sites of n class="Disease">HIV-1A17 are K103N and Y181C in RT encoding region in pol gene.

HIV-1 reverse transcriptase activity assay

Anti-RT activity of I-11 and I-12 were further evaluated by the enzymatic recombiical">nant n class="Species">HIV-1 RT activity assay (Reverse transcriptase assay kit, Roche, Germany). While I-11 showed similar anti-RT activity (IC50 = 1.09 ± 0.59 μmol/L) with NVP (IC50 = 0.92 ± 0.09 μmol/L) but lower than that of DB02 nearly three times (IC50 = 0.28 ± 0.03 μmol/L). I-12 demonstrated lower inhibitory activity (2.27 ± 0.12 μmol/L). Both two compounds have similar dose-dependent pattern in inhibiting HIV-1 RT activity with NVP and DB02 as showed in Fig. 2.

Figure 2

RT (reverse transcriptase) activity of I-11, I-12, DB02 and NVP. The figure represents three independent experiments.

RT (reverse transcriptase) activity of I-11, I-12, n class="Chemical">DB02 and NVP. The figure represents three independent experiments.

In vivo pharmacokinetics study

In vivo pharmacokinetic profile of compound I-11 was examined in Sprague–Dawley rats (Table 3 aical">nd Fig. 3). As showical">n iical">n Table 3 aical">nd Fig. 3, wheical">n administered at 5 mg/kg orally, compound I-11 was rapidly absorbed with a Tmax of 0.5 h, aloical">ng with a half-life of 2.5 h aical">nd a meaical">n residence time (MRT) of 0.554 h. The Cmax of I-11 was 654 ng/mL (1538 nmol/L), more than 400 times the EC50 value of anti-HIV-1 activity in vitro (Table 1). In this experiment, a 10.4% oral bioavailability of I-11 was also observed.

Table 3

Pharmacokinetic profile of I-11.

Parameter	i.v.a	Parameter	p.o.b
Cmax (ng/mL)	7180	Tmax (h)	0.500
t1/2 (h)	1.20	Cmax (ng/mL)	654
AUClast (h·ng/mL)	2602	t1/2 (h)	2.50
AUCINF (h·ng/mL)	2629	AUClast (h·ng/mL)	1302
MRTINF (h)	0.554	AUCINF (h·ng/mL)	1369
Vss (L/kg)	0.213	VZ/F (L/kg)	12.5
CL (L/h/kg)	0.384	CL/F (L/h/kg)	3.83
		F (%)	10.4

Dosed intravenously at 1 mg/kg.

Dosed orally at 5 mg/kg.

Figure 3

Plasma I-11 concentration-time profiles in rats following p.o. administration (5 mg/kg) and i.v. administration (1 mg/kg).

Plasma I-11 concentration-time profiles in rats followiical">ng p.o. administratioical">n (5 mg/kg) aical">nd i.v. administratioical">n (1 mg/kg).

Molecular modeling analysis

To better understand the structure-affinity relationship for this series of derivatives, the represent compounds I-01, I-11, I-23, I-35, n class="Chemical">I-38 were docked into the NNRTIs binding pocket (NNIBP, PDB code: 1RT2) compared with DB02 using the AutoDock4.2 program. Default parameters were used as described in the Sybyl-2.0 manual unless otherwise specified and the result was displayed by MOE (Molecular Operating Environment). The proposed binding modes of these compounds to the NNIBP are shown in Figure 4, Figure 5.

Figure 4

(A) Predicted binding mode of compound I-11 (purplish red) in the allosteric site of HIV-1 wild-type (WT) RT (PDB code: 1RT2) in comparison with compound DB02 (green); the docking results are showed by MOE (Molecular Operating Environment). The backbone is represented by ribbons, and amino acid residues important for binding interactions are labeled. Dotted lines show the interactions between HIV-1 RT and inhibitors. (B) The 2D-dimension representation of the interactions between the NNIBP and I-11 is presented after the docking.

Figure 5

The 2D-dimension representation of the predicted binding modes of I-01 (A), I-23 (B), I-35 (C) and I-38 (D) in the allosteric site of HIV-1 WT RT (PDB code: 1RT2).

(A) Predicted binding mode of compound I-11 (purplish red) iical">n the allosteric site of HIV-1 wild-type (WT) RT (PDB code: 1RT2) in comparison with compound DB02 (green); the docking results are showed by MOE (Molecular Operating Environment). The backbone is represented by ribbons, and amino acid residues important for binding interactions are labeled. Dotted lines show the interactions between HIV-1 RT and inhibitors. (B) The 2D-dimension representation of the interactions between the NNIBP and I-11 is presented after the docking.

The 2D-dimensioical">n representatioical">n of the predicted binn class="Gene">ding modes of I-01 (A), I-23 (B), I-35 (C) and I-38 (D) in the allosteric site of HIV-1 WT RT (PDB code: 1RT2).

Binding mode of compound I-11 iical">n the allosteric site of HIV-1 WT RT in comparison with DB02 was present at Fig. 4. Details are as follows: (1) the pyrimidine ring of DB02 and I-11 anchored into the same region of NNIBP through the H-bond formed between the 3-NH moiety on the pyrimidine ring and the main chain oxygen of Lys101. There also existed arene-H interaction between the pyrimidine ring of I-11 and the polarized CH groups of Leu100. (2) The C2 side chain of the two compounds were well accommodated in the pocket surrounded by the side chains of Lys103, Val106, Pro236, Phe227 generating potential van der Waals' interactions and electrostatic interactions to give the advantage of the affinity between inhibitor and RT. In detail, the oxygen atom of C2-β-carbonyl of DB02 and the nitrogen in the pyrazole ring of I-11 made a hydrogen-bonding interaction with the N–H function of Lys103. When compared with DB02, one additional H-bond was observed between the NH in the pyrazole ring of I-11 and the main chain oxygen of Lys103 which is good for the activity of compounds. Moreover, the terminal phenyl ring of I-11 extended to the solvent exposure region which might be the main reason of diverse groups connecting to the C5′ position of the pyrazole ring are tolerant for this region. (3) The C6-ω-cyclohexyl group of I-11 and DB02 adopted the lowest energy “chair” conformation positing in the same hydrophobic sub-pocket and exhibiting hydrophobic interactions and van der Waals′ contacts with the aromatic amino acid residues Tyr181, Tyr188 and Trp229. Compared to DB02, the cyclohexyl group of I-11 extended deeper into this region and formed arene-H with the indol ring of conserved amino acid Trp229 which might be the main reason of giving rise to the high-affinity binding to the NNBIP improving the anti-HIV-1 activity and prevented the loss of activity specifically caused by Y181C mutation.

The results of docking of I-01, I-23, I-35 aical">nd n class="Chemical">I-38 with the WT RT allosteric pocket provided additional explanation for the structure−activity relationship of these inhibitors (Fig. 5A–E). As can be seen from Fig. 5A, the interactions between compound I-01 and RT were almost identical to those of compound I-11 with RT, explains the fact that both I-01 and I-11 have good anti-HIV activity. Further analysis shows that the benzene rings at the end of C2 side chains of I-01 and I-11 reached the solvent-accessible region, the hydrophilic OH at the para-position of the benzene ring of compound I-11 was undoubtedly more favorable for the activity than the hydrophobic aromatic hydrogen of I-01, resulting lower activity of I-01.

Introduction of a chlorine atom at the C4′ position of the pyrazole ring can be seen from the results in Table 1. For most compounds, it has little effect on the activity, excepted I-23 with this value had been decreased significantly 15 folds, even though in the binding model I-01 and I-23 behaved similar (comparing Fig. 5A and B). It is worth noting that although no significant interaction between the chlorine atom and surrounding residues was observed in Fig. 5B, the introduction of a chlorine atom in compound I-23 changed the relative position between pyrazole ring and K103, thus affecting the strength of the hydrogen bond between them. The bond lengths of the two hydrogen bonds between the pyrazole ring of I-23 and K103 are longer (2.07 and 3.27 Å respectively) than that on I-01–complex (1.80 and 2.60 Å respectively), providing a rational explanation of the reduced activity of I-23, and suggesting an important role of hydrogen-bonding interactions at this position.

In addition, the results of I-35 and I-38 dockiical">ng with the allosteric pocket of WT RT (Fig. 5C aical">nd D) showed that the C5 substituent of the n class="Chemical">pyrimidine nucleus would make the inhibitor shift in NNBP, thus significantly affecting the formation of hydrogen bond between pyrazole ring and Lys103, which is considered to be important contributors to the binding affinity between RT inhibitors and enzymes. As shown in Fig. 5C, the introduction of a small methyl group at the C5 position of the pyrimidine ring (compound I-35) will move the pyrazole ring of the inhibitor to the left, cause losing the hydrogen bond between the nitrogen of pyrazole ring with the N−H of Lys103, as the result, the activity of I-35 was reduced compared with that of I-01. Also, replace 5-Me with a smaller H would cause the two hydrogen bonds between the pyrazole ring of I-38 and K103 disappeared simultaneously (Fig. 5D), which may be the reason why the efficacy of I-38 against HIV-1 decreased sharply compared with that of I-01.

In summary, the results of the auto docking analysis provided support for the SARs elucidatioical">n of our newly designed and synthesized compounds. These facts will be considered in further S-DACOs analog structural design and optimization.

Conclusions

Based on the analysis of the ligaical">nd−receptor iical">nteractions, replacement of the C2-β-carboical">nyl fragment iical">n the previously reported n class="Chemical">oxophenethyl-S-DACOs led structures with a pyrazol group resulting in the discovery of a novel series of potent NNRTIs. Most of these new congeners exhibited moderate to excellent activity against wild-type virus with an EC50 values ranging from 0.4759 to 0.0038 μmol/L. Selected compounds I-11 and I-12 displayed higher anti-HIV-1 activity against laboratory adapted strains and primary isolated strains including different subtypes and tropism strains. The results offer potential in further development of these compounds as a novel class of NNRTIs with improved antiviral efficacy and resistance profile. Furthermore, the selected compound I-11 and I-12 showed similar dose-dependent pattern in inhibiting HIV-1 RT activity with NVP confirming that the target of the compounds is RT. Compound I-11 also displayed acceptable in vivo pharmacokinetic properties and bioavailability in rats. The preliminary SARs were discussed and the molecular simulation was performed. The docking study supported our initial hypothesis that the introduction of a pyrazolyl group at C2 position of pyrimidinone could provide an additional hydrogen bond with residue in NNIBP. In addition, the C6-cyclohexyl of these title compounds point to the indole side chain of the highly conserved W229 and forms an interesting CH–π interaction which showed vital importance in improving activity against a wide range of clinically relevant HIV-1 mutations. Hence the introduction of a large hydrophobic group at the C6 position of the prymidine ring contributes to the development of new potent NNRTIs against mutant strains. Taking full advantage of the valuable information from SARs analysis, further optimization of this series of compounds aimed at improving drug resistance profiles and exploring the salient features controlling the activity are ongoing in our lab and will be reported in due course.

Experimental

Melting points (m.p.) were determined by a WRS-1 digital meltiical">ng poiical">nt instrument (Shaical">nghai, Chiical">na). n class="Chemical">1H NMR and 13C NMR spectra were obtained on a Bruker AM 400/300 MHz spectrometer (Fällanden, Switzerland) using dimethyl sulfoxide-d6 (DMSO-d6) as solvent. Chemical shifts are reported in δ (ppm) units relative to the internal reference tetramethylsilane (TMS). Mass spectra were obtained on an Agilent LC/MSD TOF mass spectrometer (Watertown, MA, USA). The regents and solvents used were all of analytical grades, and were purified and dried by standard methods before use. All air-sensitive reactions were run under nitrogen stream protection. All the reactions were monitored by thin-layer chromatography (TLC) on pre-coated silica gel G plates at 254 nm under a UV lamp using ethyl acetate/petroleum ether as eluents. Flash chromatography separations were obtained on silica gel (300–400 mesh; Qingdao Marine Chemical Factory, Qingdao, China). 5-Alkyl-6-(cyclohexylmethyl)-2-thioxo-2,3-dihydropyrimidin-4(1H)-one (4) was prepared as previously reported,.

Ethyl 2,4-dioxo-4-subtituted-butanoate (6)

Sodium metal (6.9 g, 3.0 mmol) was dissolved in absolute n class="Chemical">ethanol (40 mL). Diethyl oxalate (1.2 mmol) was added at 25 °C followed by a solution of appropriate methyl ketones 5 (1.0 mmol). The resulting mixture was stirred overnight at 25 °C. The reaction mixture was quenched by the addition of 1.0 mol/L HCl and extracted with EtOAc (2 × 40 mL). The combined organic layers were washed successively with water and brine and dried over MgSO4. After filtration and concentration in vacuo, ethyl 2,4-dioxo-4-subtituted-butanoate (6) was obtained with yields of 88%–96% which can be used in the following step without further purification.

Ethyl 5-substituted-1H-pyrazole-3-carboxylate (7)

Hydrazine hydrate (1.5 mmol) was added to a solutioical">n of n class="Chemical">ethyl 2,4-dioxo-4-subtituted-butanoate (6) (1.0 mmol) in EtOH (30 mL) and the reaction mixture was refluxed for 3 h. The reaction mixture was cool to room temperature and the solvent was evaporated under reduced pressure. The residue was poured into 25 mL water and ethyl acetate for extraction, the organic layer was washed successively with water and brine, then dried over MgSO4, filtered and concentrated in vacuo to give ethyl 5-substituted-1H-pyrazole-3-carboxylate (7) as a pale yellow solid with yields of 85%–90%, which can be used in the following step without further purification.

Ethyl 4-chloro-5-substituted-1H-pyrazole-3-carboxylate (8)

A solution of ethyl 5-substituted-1H-pyrazole-3-carboxylate (7) (1.0 mmol), n class="Chemical">N-chlorosuccinimide (NCS, 1.5 mmol) in CH3CN/DMF (10 mL/2 mL) was heated to 55 °C for 20 h. The reaction mixture was concentrated under reduced pressure and the residue was poured into 25 mL of water and extracted by ethyl acetate. The organic layer was dried over MgSO4, filtered and concentrated in vacuo to give ethyl 4-chloro-5-substituted-1H-pyrazole-3-carboxylate (8) as a light brown solid with yields of 40%–60%, which is used in the next step without further purification.

(4-Chloro-5-substituted-1H-pyrazol-3-yl)methanol (9)

LiAlH4 (1.5 mmol) was added to a 0 °C solutioical">n of n class="Chemical">ester 7 or 8 (1.0 mmol) in drying THF (10 mL). The resultant mixture was stirred at 0 °C for 2 h. The reaction was quenched by the slow addition of saturate NH4Cl. The aqueous layer was extracted with EtOAc and dried over MgSO4. After filtration and concentration in vacuo, the residue was purified by flash column chromatography or recrystallization to give alcohol 9A or 9B as a white solid with the yield of 51%–78%.

3-(Bromomethyl)-5-substituted-1H-pyrazole (10)

To a solution of alcohol 9 (1.0 mmol) iical">n n class="Chemical">CH3CN (10.0 mL) was added PBr3 (1.5 mmol) slowly. The reaction was stirred at refluxing for 2 h. The reaction mixture was then cooled to room temperature and poured into the ice water and extracted with EtOAc (2 × 40 mL). The combined organic layers were washed successively with water and brine, dried over MgSO4. After filtration and concentration in vacuo, the residue was purified by flash column chromatography to give 3-(bromomethyl)-4-chloro-5-substituted-1H-pyrazole (10) (92%–100%) as a white waxy solid.

2-(((5-Substituted-1H-pyrazol-3-yl)methyl)thio)-6-(cyclohexylmethyl)-5-alkyl-pyrimidin-4 (3H)-one I (1‒40)

K2CO3 (2.2 mmol) aical">nd compound 10 (2.2 mmol) were added to a solutioical">n of n class="Chemical">2-thiouracil (4) (2 mmol) in anhydrous DMF (8 mL). The mixture was stirred at room temperature for 8–24 h. After TLC (EtOAc/PE) revealed the disappearance of the starting material, the reaction mixture was filtered. The suspension was then diluted with cold water and extracted with ethyl acetate. The combined organic extract was washed with brine, dried with Na2SO4, and evaporated to furnish crude product, which was purified by flash chromatography or by crystallization to give the pure target compounds I (1‒40).

6-(Cyclohexylmethyl)-5-ethyl-2-(((5-phenyl-1H-pyrazol-3-yl)methyl)thio)pyrimidin-4(3H)-one (I-01)

White solid; Yield: 87.12%; m.p. 181–182 °C; 1H n class="Chemical">NMR (400 MHz, CDCl3, ppm) δ 0.85–0.88 (t, 3H, J = 8.1, CH3), 0.97–1.00 (m, 2H, cyclohexyl-H), 1.07–1.12 (m, 3H, cyclohexyl-H), 1.65‒1.69 (m, 5H, cyclohexyl-H), 1.82 (m, 1H, cyclohexyl-H), 2.46–2.48 (d, 2H, J = 6.9 Hz, CH2-cyclohexyl), 2.51–2.53 (m, 2H, CH2CH3), 4.42 (s, 2H, CH2-S), 6.52 (s, 1H, pyrazole-H), 7.34–7.36 (m, 3H, Ph-H), 7.67–7.69 (m, 2H, Ph-H), 10.24–10.73 (br, 1H, NH), 13.19 (br, 1H, NH); 13C NMR (100 MHz, CDCl3, ppm) δ 13.37, 18.75, 26.31 (2), 26.40, 26.90 (2), 33.36, 37.50, 41.80, 102.28, 122.74, 125.71 (2), 128.32, 128.86 (2), 130.56, 146.29, 146.99, 156.37, 163.11, 164.73; HR-MS: Calcd. for C23H28N4OS [M+H]+: 409.2062, Found: 409.2057.

6-(Cyclohexylmethyl)-5-ethyl-2-(((5-(m-tolyl)-1H-pyrazol-3-yl)methyl)thio)pyrimidin-4(3H)-one (I-02)

White solid; Yield: 74%; m.p. 173–175 °C; 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.92 (m, 2H, cyclohexyl-H), 0.96–1.01 (t, 3H, J=6.9 Hz, CH3), 1.08–1.14 (m, 3H, cyclohexyl-H), 1.53–1.60 (m, 5H, cyclohexyl-H), 1.80 (m, 1H, cyclohexyl-H), 2.30 (s, 3H, CH3-Ph), 2.37–2.39 (m, 2H, CH2-cyclohexyl), 2.37–2.39 (m, 2H, CH2), 4.41 (s, 2H, CH2-S), 6.55 (s, 1H, pyrazole-H), 7.06–7.09 (m, 1H, Ph-H), 7.23–7.28 (m, 1H, Ph-H), 7.50–7.54 (m, 2H, Ph-H), 12.79 (br, 2H, 2NH); 13C NMR (100 MHz, DMSO-d6, ppm) δ 13.18, 18.07, 20.92, 25.78 (3), 25.89 (2), 32.70, 36.62, 40.91, 101.48, 121.17, 122.11, 125.55, 128.31, 128.53, 130.87, 137.77, 145.19, 145.93, 156.32, 160.94, 162.91; HR-MS: Calcd. for C24H30N4OS [M+H]+: 423.2219, Found: 423.2215.

2-(((5-(3-Bromophenyl)-1H-pyrazol-3-yl)methyl)thio)-6-(cyclohexylmethyl)-5-ethyl-pyrimidin-4(3H)-one (I-03)

White solid; Yield: 74%; m.p. 182–184 °C; 1H n class="Chemical">NMR (400 MHz, CDCl3, ppm) δ 0.89–0.91 (t, 3H, J=6.0 Hz, CH3), 1.04 (m, 2H, cyclohexyl-H), 1.13 (m, 3H, cyclohexyl-H), 1.69 (m, 5H, cyclohexyl-H), 1.84 (m, 1H, cyclohexyl-H), 2.47–2.49 (m, 2H, CH2-cyclohexyl), 2.51–2.54 (m, 2H, CH2), 4.45 (s, 2H, CH2S), 6.53 (s, 1H, pyrazole-H), 7.23–7.25 (m, 1H, Ph-H), 7.41–7.44 (m, 1H, Ph-H), 7.63–7.65 (m, 1H, Ph-H), 7.90 (m, 1H, Ph-H), 12.36 (br, 1H, NH), 13.19 (br, 1H, NH); 13C NMR (100 MHz, CDCl3, ppm) δ 13.38, 18.75, 26.32 (5), 33.38, 37.58, 41.90, 102.64, 122.64, 122.92, 124.29, 128.73, 130.29, 131.00, 133.49, 144.90, 147.13, 156.42, 163.40, 164.88; HR-MS: Calcd. for C23H27BrN4OS [M+H]+: 487.1167, Found: 487.1163.

2-(((5-(3-Chlorophenyl)-1H-pyrazol-3-yl)methyl)thio)-6-(cyclohexylmethyl)-5-ethyl-pyrimidin-4(3H)-one (I-04)

White solid; Yield: 85%; m.p. 151–153 °C; 1H n class="Chemical">NMR (400 MHz, CDCl3, ppm) δ 0.97–1.01 (m, 2H, cyclohexyl-H), 1.01–1.12 (t, 3H, J = 7.2 Hz, CH3), 1.17–1.21 (m, 3H, cyclohexyl-H), 1.65–1.69 (m, 5H, cyclohexyl-H), 1.80 (m, 1H, cyclohexyl-H), 2.47–2.49 (d, 2H, J = 6.6 Hz, CH2-cyclohexyl), 2.51–2.54 (m, 2H, CH2), 4.41 (s, 2H, CH2-S), 6.50 (s, 1H, pyrazole-H), 7.25–7.30 (m, 2H, Ph-H), 7.56 (m, 1H, Ph-H), 7.58–7.70 (m, 1H, Ph-H), 12.56 (br, 1H, NH); 13C NMR (100 MHz, CDCl3, ppm) δ 3.36, 18.74, 26.13 (5), 33.37, 37.61, 41.92, 102.60, 122.69, 123.82, 125.86, 128.12, 130.05, 133.19, 134.73, 144.98, 147.21, 156.52, 163.40, 164.88; HR-MS: Calcd. for C23H27ClN4OS [M+H]+: 443.1672, Found: 443.1670.

6-(Cyclohexylmethyl)-5-ethyl-2-(((5-(3-fluorophenyl)-1H-pyrazol-3-yl)methyl)thio)pyrimidin-4(3H)-one (I-05)

White solid; Yield: 74%; m.p. 159–161 °C; 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.97–1.00 (m, 2H, cyclohexyl-H), 1.06–1.10 (t, 3H, J = 7.2 Hz, CH3), 1.13–1.16 (m, 3H, cyclohexyl-H), 1.64–1.68 (m, 5H, cyclohexyl-H), 1.80 (m, 1H, cyclohexyl-H), 2.45–2.47 (m, 2H, CH2-cyclohexyl), 2.47–2.51 (m, 2H, CH2), 4.39 (s, 2H, CH2-S), 6.49 (s, 1H, pyrazole-H), 6.96 (m, 1H, Ph-H), 7.28–7.46 (m, 3H, Ph-H), 12.40 (br, 2H, 2NH); 13C NMR (75 MHz, DMSO-d6, ppm) δ 13.32, 18.72, 26.30 (5), 33.37, 37.56, 41.90, 102.63, 112.64 (d, 2JCF = 22.6 Hz), 114.98 (d, J = 21.8), 121.33, 122.75, 130.36 (d, 3JCF = 8.1 Hz), 133.35 (d, 3JCF = 7.8 Hz), 145.29, 147.05, 156.25, 163.10 (d, 1JCF = 245.2 Hz), 163.32, 164.73; HR-MS: Calcd. for C23H27FN4OS [M+H]+: 427.1968, Found: 427.1962.

6-(Cyclohexylmethyl)-5-ethyl-2-(((5-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-yl)methyl)thio)pyrimidin-4(3H)-one (I-06)

White solid; Yield: 68%; m.p. 162–164 °C; 1H n class="Chemical">NMR (300 MHz, DMSO-d6, ppm) δ 0.75 (m, 2H, cyclohexyl-H), 0.96–0.98 (t, 3H, J = 6.6 Hz, CH3), 1.06 (m, 3H, cyclohexyl-H), 1.54 (m, 5H, cyclohexyl-H), 1.72 (m, 1H, cyclohexyl-H), 2.34–2.36 (m, 2H, cyclohexyl-H), 2.34–2.36 (m, 2H, CH2), 4.42 (s, 2H, CH2-S), 6.72 (s, CH, pyrazole-H), 7.59–7.60 (m, 2H, Ph-H), 8.01–8.0 (m, 2H, Ph-H), 12.58 (br, 2H, 2NH); 13C NMR (75 MHz, DMSO-d6, ppm) δ 13.12, 18.00, 24.94, 25.68 (2), 25.79 (2), 32.61, 36.56, 40.70, 102.09, 118.67, 121.10, 123.83, 124.08 (q, 1JCF = 270.6 Hz), 128.74, 129.63 (q, 2JCF = 31.3 Hz), 130.24, 132.89, 144.05, 146.06, 156.22, 160.68, 163.11; HR-MS: calcd. for C24H27F3N4OS [M+H]+: 477.1936, Found: 477.1932.

6-(Cyclohexylmethyl)-5-ethyl-2-(((5-(p-tolyl)-1H-pyrazol-3-yl)methyl)thio)pyrimidin-4(3H)-one (I-07)

White solid; Yield: 83%; m.p. 204–205 °C; 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.96–1.00 (t, 3H, J = 6.9 Hz, CH3), 1.11 (m, 5H, cyclohexyl-H), 1.59 (m, 5H, cyclohexyl-H), 1.80 (m, 1H, cyclohexyl-H), 2.28 (s, 3H, CH3-Ph), 2.37–2.50 (m, 2H, cyclohexyl-H), 2.37–2.50 (m, 2H, CH2), 4.39 (s, 2H, CH2-S), 6.53 (s, 1H, pyrazole-H), 7.17–7.19 (m, 2H, Ph-H), 7.58–7.61 (m, 2H, Ph-H), 12.77 (br, 2H, 2NH); 13C NMR (100 MHz, DMSO-d6, ppm) δ 13.21, 18.05, 20.70, 25.77 (3), 25.88 (2), 32.70, 36.59, 40.89, 101.20, 121.16, 124.86 (2), 128.09, 129.23 (2), 137.01, 142.32, 145.67, 156.31, 160.94, 162.89.162.89; HR-MS: Calcd. for C24H30N4OS [M+H]+: 423.2219, Found: 423.2217.

2-(((5-(4-Chlorophenyl)-1H-pyrazol-3-yl)methyl)thio)-6-(cyclohexylmethyl)-5-ethyl-pyrimidin-4(3H)-one (I-08)

White solid; Yield: 88%; m.p. 203–204 °C; 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.97–0.99 (t, 3H, J = 7.2 Hz, CH3), 1.09 (m, 2H, cyclohexyl-H), 1.18 (m, 3H, cyclohexyl-H), 1.57 (m, 5H, cyclohexyl-H), 1.75 (m, 1H, cyclohexyl-H), 2.35–2.39 (m, 2H, CH2-cyclohexyl), 2.35–2.39 (m, 2H, CH2), 4.39 (s, 2H, CH2-S), 6.58–6.61 (s, 1H, pyrazole-H), 7.28–7.45 (m, 2H, Ph-H), 7.69–7.75 (m, 2H, Ph-H), 12.78 (br, 1H, NH); 13C NMR (100 MHz, DMSO-d6, ppm) δ 13.21, 18.03, 25.73 (2), 25.86 (3), 32.65, 36.58, 40.76, 101.71, 121.98, 124.93, 126.61, 127.66, 128.68, 131.86, 132.08, 145.91, 156.29, 160.85, 163.02; HR-MS: Calcd. for C23H27ClN4OS [M+H]+: 443.1672, Found: 443.1668.

6-(Cyclohexylmethyl)-5-ethyl-2-(((5-(4-fluorophenyl)-1H-pyrazol-3-yl)methyl)thio)pyrimidin-4(3H)-one (I-09)

White solid; Yield: 89%; m.p. 174–176 °C. 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.90 (m, 2H, cyclohexyl-H), 0.95–0.99 (t, 3H, J = 7.5 Hz, CH3), 1.06–1.09 (m, 3H, cyclohexyl-H), 1.56–1.60 (m, 5H, cyclohexyl-H), 1.75 (m, 1H, cyclohexyl-H), 2.36–2.38 (m, 2H, CH2-cyclohexyl), 2.36–2.38 (m, 2H, CH2), 4.39 (s, 2H, CH2-S), 6.57 (s, 1H, pyrazole-H), 7.18–7.24 (m, 2H, Ph-H), 7.73–7.77 (m, 2H, Ph-H), 12.78 (br, 1H, NH); 13C NMR (DMSO-d6, 75 MHz) δ 13.18, 18.02, 25.40 (2), 25.72, 25.85 (2), 32.65, 36.58, 40.71, 101.55, 115.53 (2C, d, 2JCF = 21.4 Hz), 121.07, 126.95 (2C, d, 3JCF = 8.0 Hz), 127.99, 144.42, 145.63, 156.32, 160.62, 161.64 (d, 1JCF = 243.0 Hz), 163.10; HR-MS: Calcd. for C23H27FN4OS [M+H]+: 427.1968, Found: 427.1964.

6-(Cyclohexylmethyl)-5-ethyl-2-(((5-(4-methoxyphenyl)-1H-pyrazol-3-yl)methyl)thio)pyrimidin-4(3H)-one (I-10)

White solid; Yield: 50%; m.p. 283–284 °C; 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.95–1.00 (t, 3H, J = 7.2 Hz, CH3), 1.02–1.12 (m, 2H, cyclohexyl-H), 1.16–1.20 (m, 3H, cyclohexyl-H), 1.59–1.62 (m, 5H, cyclohexyl-H), 1.78 (m, 1H, cyclohexyl-H), 2.36–2.38 (m, 2H, CH2-cyclohexyl), 2.38–2.40 (m, 2H, CH2), 3.76 (s, 3H, OCH3), 4.37 (s, 2H, CH2-S), 6.49 (s, 1H, pyrazole-H), 6.95–6.98 (m, 2H, Ph-H), 7.62–7.65 (m, 2H, Ph-H), 12.07–13.10 (br, 1H, NH); 13C NMR (100 MHz, DMSO-d6, ppm) δ 13.23, 18.02, 25.74 (2), 25.88 (3), 32.66, 36.58, 40.71, 55.06, 100.85, 114.11 (2), 121.10, 123.45, 126.31 (2), 142.23, 145.48, 156.39, 158.90, 160.65, 163.03; HR-MS: Calcd. for C24H30N4O2S [M+H]+: 439.2168, Found: 439.2164.

6-(Cyclohexylmethyl)-5-ethyl-2-(((5-(4-hydroxyphenyl)-1H-pyrazol-3-yl)methyl)thio)pyrimidin-4(3H)-one (I-11)

White solid; Yield: 54.59%; m.p. 228–230 °C; 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.96–1.00 (t, 3H, J = 7.2 Hz, CH3), 1.09–1.12 (m, 5H, cyclohexyl-H), 1.59–1.62 (m, 5H, cyclohexyl-H), 1.79 (m, 1H, cyclohexyl-H), 2.36–2.40 (m, 2H, CH2-cyclohexyl), 2.36–2.40 (m, 2H, CH2), 4.36 (s, 2H, CH2-S), 6.42 (s, 1H, pyrazole-H), 6.78–6.81 (m, 2H, Ph-H), 7.50–7.53 (m, 2H, Ph-H), 9.62 (br, 1H, OH), 12.67 (br, 2H, 2NH); 13C NMR (100 MHz, DMSO-d6, ppm) δ 13.22, 18.04, 25.75, 25.88 (2), 26.07 (2), 32.67, 36.59, 40.76, 100.49, 115.48 (2), 121.42, 126.40 (2), 128.38, 145.64, 156.39, 157.25 (2), 160.82, 162.98; HR-MS: Calcd. for C23H28N4O2S [M+H]+: 425.2011, Found: 425.2008.

6-(Cyclohexylmethyl)-5-ethyl-2-(((5-(4-(methylthio)phenyl)-1H-pyrazol-3-yl)methyl)thio)pyrimidin-4(3H)-one (I-12)

White solid; Yield: 53%; m.p. 226–228 °C; 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.96–1.00 (t, 3H, J = 7.2 Hz, CH3), 1.08–1.10 (m, 2H, cyclohexyl-H), 1.15 (m, 3H, cyclohexyl-H), 1.58 (m, 5H, cyclohexyl-H), 1.78 (m, 1H, cyclohexyl-H), 2.37 (s, 3H, CH3S), 2.46–2.50 (m, 2H, CH2-cyclohexyl), 2.46–2.50 (m, 2H, CH2), 4.40 (s, 2H, CH2-S), 6.55 (s, 1H, pyrazole-H), 7.25–7.28 (m, 2H, Ph-H), 7.64–7.67 (m, 2H, Ph-H), 12.78 (br, 2H, 2NH); 13C NMR (100 MHz, DMSO-d6, ppm) δ 13.22, 14.52, 18.05, 25.77 (3), 25.89 (2), 32.69, 36.58, 40.88, 101.32, 121.15, 125.41 (2), 125.99 (2), 127.65, 137.69, 145.61, 156.25, 160.98, 162.90; HRMS (ESI-TOF): Calcd. for C24H30N4OS2 [M+H]+: 455.1939, Found: 455.1935.

6-(Cyclohexylmethyl)-5-ethyl-2-(((5-(4-isopropylphen-yl)-1H-pyrazol-3-yl)methyl)thio)pyrimidin-4(3H)-one (I-13)

White solid; Yield: 72%; m.p. 207–209 °C; 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.92 (m, 2H, cyclohexyl-H), 0.96–1.01 (t, 3H, J = 7.5 Hz, CH3), 1.08–1.10 (d, 6H, J = 7.5 Hz, 2CH3), 1.15–1.17 (m, 3H, cyclohexyl-H), 1.59 (m, 5H, cyclohexyl-H), 1.80 (m, 1H, cyclohexyl-H), 2.37–2.39 (m, 2H, CH2-cyclohexyl), 2.37–2.39 (m, 2H, CH2), 2.81–2.85 (m, 1H, CHMe2), 4.40 (s, 2H, CH2-S), 6.53 (s, 1H, pyrazole-H), 7.21–7.24 (m, 2H, Ph-H), 7.60–7.63 (m, 2H, Ph-H), 12.78 (br, 1H, NH); 13C NMR (100 MHz, DMSO-d6, ppm) δ 13.19, 18.06, 23.62 (2), 25.77 (3), 25.89, 25.89, 32.71, 33.16, 36.59, 40.92, 101.29, 121.15, 125.00 (2), 126.53 (2), 126.53, 128.49, 145.66, 147.92, 156.35, 160.92, 162.90; HR-MS: Calcd. for C26H34N4OS [M+H]+: 451.2532, Found: 451.2529.

6-(Cyclohexylmethyl)-2-(((5-(3,4-dichlorophenyl)-1H-pyrazol-3-yl)methyl)thio)-5-ethyl-pyrimidin-4(3H)-one (I-14)

White solid; Yield: 52%; m.p. 309–311 °C; 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.94 (m, 2H, cyclohexyl-H), 0.96–0.99 (t, 3H, J = 7.2 Hz, CH3), 1.05–1.08 (m, 3H, cyclohexyl-H), 1.55–1.58 (m, 5H, cyclohexyl-H), 1.72 (m, 1H, cyclohexyl-H), 2.35–2.37 (m, 2H, CH2-cyclohexyl), 2.35–2.37 (m, 2H, CH2), 4.39 (s, 2H, CH2-S), 6.68 (s, 1H, pyrazole-H), 7.59–7.62 (m, 1H, Ph-H), 7.68–7.72 (m, 1H, Ph-H), 7.94–7.95 (m, 1H, Ph-H), 12.81 (br, 2H, 2NH); 13C NMR (100 MHz, DMSO-d6, ppm) δ 13.19, 18.02, 24.90, 25.72 (2), 25.86 (2), 32.63, 36.58, 40.82, 102.26, 121.06, 124.96, 126.47 (2), 129.78, 130.82, 131.53, 141.48, 144.69, 156.09, 161.07, 162.99; HR-MS: Calcd. for C23H26Cl2N4OS [M+H]+: 477.1283, Found: 477.1279.

6-(Cyclohexylmethyl)-2-(((5-(3,4-difluorophenyl)-1H-pyrazol-3-yl)methyl)thio)-5-ethyl-pyrimidin-4(3H)-one (I-15)

White solid; Yield: 90%; m.p. 190–191 °C; 1H n class="Chemical">NMR (300 MHz, DMSO-d6, ppm) δ 0.94–0.99 (t, 3H, J = 7.2 Hz, CH3), 1.05–1.08 (m, 5H, cyclohexyl-H), 1.55–1.59 (m, 5H, cyclohexyl-H), 1.72 (m, 1H, cyclohexyl-H), 2.32–2.38 (m, 2H, CH2-cyclohexyl), 2.32–2.38 (m, 2H, CH2), 4.38 (s, 2H, CH2-S), 6.64 (s, 1H, pyrazole-H), 7.38–7.45 (m, 1H, Ph-H), 7.48–7.59 (m, 1H, Ph-H), 7.71–7.78 (m, 1H, Ph-H), 11.81–12.97 (br, 2H, 2NH); 13C NMR (75 MHz, DMSO-d6, ppm) δ 13.16, 18.01, 24.98, 25.70 (2), 25.84 (2), 32.62, 36.59, 40.71, 102.03, 113.8 (d, 2JCF = 18.2 Hz), 117.78 (d, 2JCF = 17.3 Hz), 121.04, 121.63, 129.61, 143.99, 145.48, 148.89 (dd, 1JCF = 244.5, 2JCF = 12.4 Hz), 149.72 (dd, 1JCF = 243.3, 2JCF = 12.3 Hz), 156.27, 160.66, 163.12; HR-MS: Calcd. for C23H26F2N4OS [M+H]+: 445.1874, Found: 445.1871.

6-(Cyclohexylmethyl)-2-(((5-(2,4-dimethylphenyl)-1H-pyrazol-3-yl)methyl)thio)-5-ethyl-pyrimidin-4(3H)-one (I-16)

White solid; Yield: 65%; m.p. 175–177 °C; 1H n class="Chemical">NMR (DMSO-d6, 400 MHz, ppm) δ 0.96–1.00 (m, 2H, cyclohexyl-H), 1.04–1.09 (t, 3H, J = 7.2 Hz, CH3), 1.17–1.25 (m, 3H, cyclohexyl-H), 1.65 (m, 5H, cyclohexyl-H), 1.84 (m, 1H, cyclohexyl-H), 2.31 (s, 3H, CH3-Ph), 2.35 (s, 3H, CH3-Ph), 2.44–2.50 (m, 2H, CH2-cyclohexyl), 2.44–2.50 (m, 2H, CH2), 4.46 (s, 2H, CH2-S), 6.35 (s, CH, pyrazole-H), 6.97–7.03 (m, 2H, Ph-H), 7.30–7.32 (m, 2H, Ph-H), 12.54 (br, 2H, NH); 13C NMR (100 MHz, DMSO-d6, ppm) δ 13.40, 18.77, 20.86, 21.23, 26.37, 26.50 (2), 27.51 (2), 33.43, 37.43,41.86, 105.16, 122.78, 126.82, 127.29, 129.03, 131.70, 135.80, 138.29, 145.35, 146.58, 156.26, 163.03, 164.67; HR-MS: Calcd. for C25H32N4OS [M+H]+: 437.2375, Found: 437.2372.

6-(Cyclohexylmethyl)-2-(((5-(2,4-difluorophenyl)-1H-pyrazol-3-yl)methyl)thio)-5-ethyl-pyrimidin-4(3H)-one (I-17)

White solid; Yield: 75%; m.p. 207–209 °C; 1H n class="Chemical">NMR (400 MHz, CDCl3, ppm) δ 1.07–1.10 (m, 2H, cyclohexyl-H), 1.12–1.17 (t, 3H, J = 6.9 Hz, CH3), 1.22–1.26 (m, 3H, cyclohexyl-H), 1.66–1.70 (m, 5H, cyclohexyl-H), 1.82 (m, 1H, cyclohexyl-H), 2.48–2.56 (m, 2H, CH2-cyclohexyl), 2.48–2.56 (m, 2H, CH2), 4.44 (s, 2H, CH2-S), 6.62 (s, 1H, pyrazole-H), 6.85–6.92 (m, 2H, Ph-H), 7.76–7.7 (m, 1H, Ph-H), 12.09–12.42 (br, 2H, 2NH); 13C NMR (75 MHz, CDCl3, ppm) δ 13.33, 18.72, 26.29 (3), 26.38 (2), 33.36, 37.54, 41.90, 104.71 (dd, 2JCF = 17.5, 10.8 Hz), 111.90 (d, 2JCF = 21.2 Hz), 115.54 (d, 2JCF = 12.8 Hz), 122.75, 129.12, 141.28, 145.26, 156.33, 157.92, 159.67 (dd, 1JCF = 250.6 Hz, 3JCF = 11.8 Hz), 162.51 (dd, 1JCF = 248.8, 3JCF = 11.9 Hz), 163.22, 164.79; HR-MS: Calcd. for C23H26F2N4OS [M+H]+: 445.1874, Found: 445.1872.

6-(Cyclohexylmethyl)-5-ethyl-2-(((5-(pyridin-2-yl)-1H-pyrazol-3-yl)methyl)thio)pyrimidin-4(3H)-one (I-18)

White solid; Yield: 30%; m.p. 140–142 °C; 1H n class="Chemical">NMR (400 MHz, CDCl3, ppm) δ 1.00–1.04 (m, 2H, cyclohexyl-H), 1.07–1.12 (t, 3H, J = 7.5 Hz, CH3), 1.16–1.26 (m, 3H, cyclohexyl-H), 1.63–1.69 (m, 5H, cyclohexyl-H), 1.80–1.83 (m, 1H, cyclohexyl-H), 2.45–2.47 (d, 2H, J = 6.9 Hz, CH2-cyclohexyl), 2.51–2.54 (m, 2H, CH2), 4.49 (s, 2H, CH2-S), 6.75 (s, 1H, pyrazole-H), 7.20–7.21 (m, 1H, pyridine-H), 7.69 (m, 2H, pyridine-H), 8.63–8.64 (m, 1H, pyridine-H), 10.27–10.67 (br, 2H, 2NH); 13C NMR (100 MHz, CDCl3, ppm) δ 13.32, 18.67, 26.28 (2), 26.39, 27.08, 27.08, 33.35, 37.38, 41.76, 101.19, 120.31, 122.69, 122.92, 137.01, 145.22, 147.74, 148.91, 149.41, 156.10, 161.87, 164.82; HR-MS: Calcd. for C22H27N5OS [M+H]+: 410.2015, Found: 410.2012.

2-(((1H-Pyrazol-3-yl)methyl)thio)-6-(cyclohexyl-methyl)-5-ethylpyrimidin-4(3H)-one (I-19)

White solid; Yield: 52%; m.p. 162–164 °C; 1H n class="Chemical">NMR (400 MHz, CDCl3, ppm) δ 1.05 (m, 2H, cyclohexyl-H), 1.07–1.12 (t, 3H, J = 7.5 Hz, CH3), 1.17–1.26 (m, 3H, cyclohexyl-H), 1.66–1.70 (m, 5H, cyclohexyl-H), 1.80–1.85 (m, 1H, cyclohexyl-H), 2.45–2.47 (d, 2H, J = 7.2 Hz, CH2-cyclohexyl), 2.51–2.53 (m, 2H, CH2), 4.44 (s, 2H, CH2-S), 6.27–6.28 (d, 1H, J = 7.5 Hz, pyrazole-H), 7.52–7.53 (d, J = 7.5 Hz, 1H, pyrazole-H), 10.90–10.95 (br, 2H, 2NH); 13C NMR (100 MHz, CDCl3, ppm) δ 13.28, 18.71, 26.31 (2), 26.41, 27.19 (2), 33.34, 37.36, 41.74, 104.76, 122.66, 131.71, 140.92, 145.81, 156.11, 163.09, 164.65; HR-MS: Calcd. for C17H24N4OS [M+H]+: 333.1749, Found: 333.1747.

6-(Cyclohexylmethyl)-5-ethyl-2-(((5-methyl-1H-pyrazol-3-yl)methyl)thio)pyrimidin-4(3H)-one (I-20)

White solid; Yield: 49%; m.p. 175–176 °C; 1H n class="Chemical">NMR (400 MHz, CDCl3, ppm) δ 1.04 (m, 2H, cyclohexyl-H), 1.07–1.12 (t, 3H, J = 7.5 Hz, CH3), 1.16–1.23 (m, 3H, cyclohexyl-H), 1.66–1.69 (m, 5H, cyclohexyl-H), 1.80–1.85 (m, 1H, cyclohexyl-H), 2.30 (s, 3H, CH3-pyrazole), 2.44–2.46 (d, 2H, J = 7.2 Hz, CH2-cyclohexyl), 2.50–2.53 (m, 2H, CH2), 4.37 (s, 2H, CH2-S), 6.02 (s, 1H, pyrazole-H), 11.01–11.45 (br, 2H, NH); 13C NMR (100 MHz, CDCl3, ppm) δ 11.48, 13.30, 18.68, 26.31, 26.31 (2), 27.33 (2), 33.33, 37.33, 41.72, 104.13, 122.59, 141.94, 146.81, 156.33, 163.02, 164.63; HR-MS: Calcd. for C18H26N4OS [M+H]+: 347.1906, Found: 347.1904.

6-(Cyclohexylmethyl)-2-(((5-cyclopropyl-1H-pyrazol-3-yl)methyl)thio)-5-ethyl-pyrimidin-4(3H)-one (I-21)

White solid; Yield: 38%; m.p. 168–171 °C; 1H n class="Chemical">NMR (400 MHz, CDCl3, ppm) δ 1.04 (m, 2H, cyclohexyl-H), 1.07–1.12 (t, 3H, J = 7.5 Hz, CH3), 1.17–1.20 (m, 4H, 2 × CH2), 1.23–1.26 (m, 3H, cyclohexyl-H), 1.66–1.69 (m, 5H, cyclohexyl-H), 1.82 (m, 1H, cyclohexyl-H), 1.87–1.94 (m, 1H, cyclopropyl-H), 2.43–2.50 (d, 2H, J = 7.5 Hz, CH2-cyclohexyl), 2.53–2.55 (m, 2H, CH2), 4.36 (s, 2H, CH2-S), 5.88 (s, 1H, pyrazole-H), 11.72 (br, 2H, NH); 13C NMR (100 MHz, CDCl3, ppm) δ 7.37, 7.87 (2), 13.31, 18.69, 26.32 (2), 26.43, 27.26 (2), 33.34, 37.36, 41.75, 100.94, 122.59, 146.25, 149.44, 156.37, 163.01, 164.63; HR-MS: Calcd. for C20H28N4OS [M+H]+: 373.2062, Found: 373.2060.

2-(((5-(tert-Butyl)-1H-pyrazol-3-yl)methyl)thio)-6-(cyclohexylmethyl)-5-ethyl-pyrimidin-4(3H)-one (I-22)

White solid; Yield: 45%; m.p. 153–154 °C; 1H n class="Chemical">NMR (400 MHz, CDCl3, ppm) δ 0.85 (m, 2H, cyclohexyl-H), 1.02 (s, 9H, 3× CH3), 1.07–1.12 (t, 3H, J = 7.5 Hz, CH3), 1.34 (m, 1H, cyclohexyl-H), 1.67−1.71 (m, 7H, cyclohexyl-H), 1.84 (m, 1H, cyclohexyl-H), 2.44–2.47 (d, 2H, J = 6.9 Hz, CH2-cyclohexyl), 2.51–2.56 (m, 2H, CH2), 4.38 (s, 2H, CH2-S), 6.05 (s, 1H, pyrazole-H), 12.68 (br, 2H, NH); 13C NMR (100 MHz, CDCl3, ppm) δ 13.32, 18.70, 26.34 (2), 26.44, 28.01 (2), 30.26 (3), 31.23, 33.35, 37.35, 41.76, 100.91, 122.76, 142.18, 146.33, 155.36, 156.21, 162.80, 164.32; HR-MS: Calcd. for C21H32N4OS [M+H]+: 389.2375, Found: 389.2370.

2-(((4-Chloro-5-phenyl-1H-pyrazol-3-yl)methyl)thio)-6-(cyclohexylmethyl)-5-ethyl-pyrimidin-4(3H)-one (I-23)

White solid; Yield: 86%; m.p. 233–234 °C; 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.94–0.97 (t, 3H, J = 7.2 Hz, CH3), 0.99 (m, 2H, cyclohexyl-H), 1.06–1.08 (m, 3H, cyclohexyl-H), 1.56–1.59 (m, 5H, cyclohexyl-H), 1.81 (m, 1H, cyclohexyl-H), 2.32–2.34 (d, 2H, J = 6.0 Hz, CH2-cyclohexyl), 2.36–2.38 (m, 2H, CH2), 4.44 (s, 2H, CH2-S), 7.40–7.51 (m, 3H, Ph-H), 7.77–7.79 (m, 2H, Ph-H), 10.88 (br, 1H, NH), 13.19 (br, 1H, NH); 13C NMR (100 MHz, DMSO-d6, ppm) δ 13.19, 18.01, 25.00 (2), 25.73 (2), 25.87, 32.63, 36.53, 40.76, 105.07, 121.05, 126.42, 126.42, 126.42, 128.67 (3), 140.28, 141.02, 155.86, 161.05, 162.92; HR-MS: Calcd. for C23H27ClN4OS [M+H]+: 443.1672, Found: 443.1666.

2-(((4-Chloro-5-(m-tolyl)-1H-pyrazol-3-yl)methyl)thio)-6-(cyclohexylmethyl)-5-ethyl-pyrimidin-4(3H)-one (I-24)

White solid; Yield: 67%; m.p. 218–219 °C; 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.89 (m, 2H, cyclohexyl-H), 0.95–0.99 (t, 3H, J = 7.2 Hz, CH3), 1.08 (m, 3H, cyclohexyl-H), 1.56–1.59 (m, 5H, cyclohexyl-H), 1.79 (m, 1H, cyclohexyl-H), 2.30 (s, 3H, CH3-Ph), 2.33–2.37 (m, 2H, CH2-cyclohexyl), 2.33–2.37 (m, 2H, CH2), 4.45 (s, 2H, CH2-S), 7.17–7.19 (m, 1H, Ph-H), 7.31–7.36 (m, 1H, Ph-H), 7.57–7.59 (m, 2H, Ph-H), 12.95 (br, 2H, 2NH); 13C NMR (100 MHz, DMSO-d6, ppm) δ 13.16, 18.02, 20.97, 23.97, 25.76 (2), 25.90 (2), 32.67, 36.52, 40.79, 105.08, 121.10, 123.60, 126.98 (2), 128.52, 129.03, 137.81, 141.98, 142.53, 156.03, 161.03, 162.94; HR-MS: Calcd. for C24H29ClN4OS [M+H]+: 457.1829, Found: 457.1826.

2-(((5-(3-Bromophenyl)-4-chloro-1H-pyrazol-3-yl)methyl)thio)-6-(cyclohexylmethyl)-5-ethyl-pyrimidin-4(3H)-one (I-25)

White solid; Yield: 90%; m.p. 236–237 °C; 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.93–0.95 (t, 3H, J = 7.2 Hz, CH3), 0.98 (m, 2H, cyclohexyl-H), 1.02–1.04 (m, 3H, cyclohexyl-H), 1.52–1.55 (m, 5H, cyclohexyl-H), 1.71 (m, 1H, cyclohexyl-H), 2.33–2.35 (m, 2H, CH2-cyclohexyl), 2.33–2.35 (m, 2H, CH2), 4.43 (s, 2H, CH2-S), 7.42–7.45 (m, 1H, Ph-H), 7.55–7.58 (m, 1H, Ph-H), 7.80–7.82 (m, 1H, Ph-H), 7.95 (m, 1H, Ph-H), 13.01 (br, 2H, 2NH); 13C NMR (100 MHz, DMSO-d6, ppm) δ 13.58, 18.26, 23.13, 25.77 (4), 32.73, 36.87, 40.65, 104.93, 119.02, 121.85, 125.25, 128.74, 130.77 (2), 132.99, 140.95, 141.24, 160.40, 161.32, 168.14; HR-MS: Calcd. for C23H26BrClN4OS [M+H]+: 521.0777, Found: 521.0773.

2-(((4-Chloro-5-(3-chlorophenyl)-1H-pyrazol-3-yl)methyl)thio)-6-(cyclohexylmethyl)-5-ethyl-pyrimidin-4(3H)-one (I-26)

White solid; Yield: 90.02%; m.p. 217–219 °C; 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.88 (m, 2H, cyclohexyl-H), 0.93–0.98 (t, 3H, J = 7.2 Hz, CH3), 1.03–1.05 (m, 3H, cyclohexyl-H), 1.53–1.56 (m, 5H, cyclohexyl-H), 1.72 (m, 1H, cyclohexyl-H), 2.34–2.36 (m, 2H, CH2-cyclohexyl), 2.34–2.36 (m, 2H, CH2), 4.43 (s, 2H, CH2-S), 7.44–7.53 (m, 2H, Ph-H), 7.76–7.81 (m, 2H, Ph-H), 12.50–13.54 (br, 2H, NH); 13C NMR (100 MHz, DMSO-d6, ppm) δ 13.26, 18.05, 23.37, 25.72 (2), 25.86 (2), 32.62 (2), 40.65, 105.34, 120.48, 124.85, 125.89, 128.02, 130.52, 131.97, 133.39, 140.52, 141.12, 157.09, 161.09, 164.33; HR-MS: Calcd. for C23H26Cl2N4OS [M+H]+: 477.1283, Found: 477.1279.

2-(((4-Chloro-5-(3-fluorophenyl)-1H-pyrazol-3-yl)methyl)thio)-6-(cyclohexylmethyl)-5-ethyl-pyrimidin-4(3H)-one (I-27)

White solid; Yield: 89%; m.p. 226–227 °C; 1H n class="Chemical">NMR (300 MHz, DMSO-d6, ppm) δ 0.95 (m, 3H, CH3), 1.04 (m, 5H, cyclohexyl-H), 1.53 (m, 5H, cyclohexyl-H), 1.72 (m, 1H, cyclohexyl-H), 2.20–2.45 (m, 2H, CH2-cyclohexyl), 2.20–2.45 (m, 2H, CH2), 4.43 (s, 2H, CH2-S), 7.22 (m, 1H, Ph-H), 7.55–7.65 (m, 3H, Ph-H), 12.98–13.05 (br, 2H, 2NH); 13C NMR (75 MHz, DMSO-d6, ppm) δ 13.15, 17.99, 23.55, 25.70 (2), 25.84 (2), 32.59, 36.52, 40.76, 105.46 (2), 112.88 (d, 2JCF = 22.9 Hz), 115.10 (d, 2JCF = 21.2 Hz), 121.10, 122.35 (2C), 130.77 (d, 3JCF = 7.7 Hz), 143.31, 155.95, 161.11, 162.12 (d, 1JCF = 242.2 Hz), 162.95; HR-MS: Calcd. for C23H26ClFN4OS [M+H]+: 461.1578, Found: 461.1576.

2-(((4-Chloro-5-(3-(trifluoromethyl)phenyl)-1H- pyrazol-3-yl)methyl)thio)-6-(cyclohexyl-methyl)-5-ethyl-pyrimidin-4(3H)-one (I-28)

White solid; Yield: 64%; m.p. 198–199 °C; 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 1.68–1.81 (m, 2H, cyclohexyl-H), 1.85–1.90 (t, 3H, J = 6.9 Hz, CH3), 1.93 (m, 3H, cyclohexyl-H), 2.06–2.45 (m, 5H, cyclohexyl-H), 2.62 (m, 1H, cyclohexyl-H), 3.25–3.27 (m, 2H, cyclohexyl-H), 3.25–3.27 (m, 2H, CH2), 5.37 (s, 2H, CH2-S), 8.52–8.64 (m, 2H, Ph-H), 8.93–9.04 (m, 2H, Ph-H), 13.94 (br, 2H, 2NH); 13C NMR (75 MHz, DMSO-d6, ppm) δ 13.10, 17.98, 23.36, 25.66 (2), 25.79 (2), 32.58, 36.51, 40.76, 105.41, 105.54, 121.09, 122.58, 123.94 (q, 1JCF = 270.8 Hz), 124.66, 128.66, 129.33, 129.48 (q, 2JCF = 27.6 Hz), 129.79, 130.04, 155.81, 161.05, 162.95; HR-MS: Calcd. for C24H26ClF3N4OS [M+H]+: 511.1546, Found: 511.1545.

2-(((4-Chloro-5-(p-tolyl)-1H-pyrazol-3-yl)methyl)thio)-6-(cyclohexylmethyl)-5-ethyl-pyrimidin-4(3H)-one (I-29)

White solid; Yield: 63%; m.p. 225–226 °C; 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.97–0.99 (m, 2H, cyclohexyl-H), 1.06–1.12 (t, 3H, J = 7.8 Hz, CH3), 1.17 (m, 3H, cyclohexyl-H), 1.52–1.59 (m, 5H, cyclohexyl-H), 1.79 (m, 1H, cyclohexyl-H), 2.31 (s, 3H, CH3-Ph), 2.35–2.37 (m, 2H, CH2-cyclohexyl), 2.35–2.37 (m, 2H, CH2), 4.44 (s, 2H, CH2-S), 7.25–7.28 (m, 2H, Ph-H), 7.65–7.6 (m, 2H, Ph-H), 12.94 (br, 2H, 2NH); 13C NMR (100 MHz, DMSO-d6, ppm) δ 13.17, 18.00, 20.76, 24.04, 25.76 (2), 25.89 (2), 32.66, 36.51, 40.76, 104.82, 121.10, 126.68 (3), 129.22 (2), 137.95, 140.17, 141.01, 156.00, 161.02, 162.93; HR-MS: Calcd. for C24H29ClN4OS [M+H]+: 457.1829, Found: 457.1826.

2-(((4-Chloro-5-(4-chlorophenyl)-1H-pyrazol-3-yl)methyl)thio)-6-(cyclohexylmethyl)-5-ethyl-pyrimidin-4(3H)-one (I-30)

White solid; Yield: 90%; m.p. 224–226 °C; 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.88 (m, 2H, cyclohexyl-H), 0.93–0.98 (t, 3H, J = 7.2 Hz, CH3), 1.03–1.05 (m, 3H, cyclohexyl-H), 1.53–1.56 (m, 5H, cyclohexyl-H), 1.74 (m, 1H, cyclohexyl-H), 2.34–2.36 (m, 2H, CH2-cyclohexyl), 2.34–2.36 (m, 2H, CH2), 4.43 (s, 2H, CH2-S), 7.52–7.55 (m, 2H, Ph-H), 7.79–7.81 (m, 2H, Ph-H), 13.01 (br, 2H, 2NH); 13C NMR (100 MHz, DMSO-d6, ppm) δ 13.18, 17.99, 23.53, 25.71 (2), 25.85 (2), 32.60, 36.52, 40.75, 105.26, 121.09, 128.03 (3), 128.76 (2), 133.06, 140.46, 141.02, 155.78, 161.11, 162.93; HR-MS: Calcd. for C23H26Cl2N4OS [M+H]+: 477.1278, Found: 477.1275.

2-(((4-Chloro-5-(4-fluorophenyl)-1H-pyrazol-3-yl)methyl)thio)-6-(cyclohexylmethyl)-5-ethyl-pyrimidin-4(3H)-one (I-31)

White solid; Yield: 71%; m.p. 310–312 °C; 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.96–0.98 (m, 2H, cyclohexyl-H), 1.04–1.06 (t, 3H, J = 7.8 Hz, CH3), 1.13–1.15 (m, 3H, cyclohexyl-H), 1.50–1.58 (m, 5H, cyclohexyl-H), 1.75 (m, 1H, cyclohexyl-H), 2.35–2.37 (m, 2H, CH2-cyclohexyl), 2.35–2.37 (m, 2H, CH2), 4.43 (s, 2H, CH2-S), 7.29–7.35 (m, 2H, Ph-H), 7.79–7.84 (m, 2H, Ph-H), 12.96 (br, 2H, 2NH); 13C NMR (75 MHz, DMSO-d6, ppm) δ 13.17, 17.99, 23.69, 25.72 (2), 25.86 (2), 32.61, 36.51, 40.75, 104.97 (2), 115.67 (2C, d, 2JCF = 21.5 Hz), 121.11, 125.93, 128.60 (2C, d, 3JCF = 8.2 Hz), 141.36, 155.92, 161.08, 161.96 (d, 1JCF = 244.7 Hz), 162.95; HR-MS: Calcd. for C23H26ClFN4OS [M+H]+: 461.1578, Found: 461.1575.

2-(((4-Chloro-5-(3,4-dichlorophenyl)-1H-pyrazol-3-yl)methyl)thio)-6-(cyclohexylmethyl)-5-ethyl-pyrimidin-4(3H)-one (I-32)

White solid; Yield: 83%; m.p. 227–228 °C; 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.93-0.98 (t, 3H, J = 7.2 Hz, CH3), 1.04 (m, 5H, cyclohexyl-H), 1.53–1.55 (m, 5H, cyclohexyl-H), 1.70 (m, 1H, cyclohexyl-H), 2.33–2.36 (m, 2H, CH2-cyclohexyl), 2.33–2.36 (m, 2H, CH2), 4.42 (s, 2H, CH2S), 7.46–7.55 (m, 2H, Ph-H), 7.98–7.99 (m, 1H, Ph-H), 12.72–13.36 (br, 2H, 2NH); 13C NMR (100 MHz, DMSO-d6, ppm) δ 13.17, 17.99, 23.17, 25.70 (2), 25.84 (2), 32.59, 36.52, 40.76, 105.57, 121.09, 124.86, 126.07, 128.73, 130.91, 131.49, 133.42, 140.68, 141.77, 155.74, 161.08, 162.96; HR-MS: Calcd. for C23H26Cl3N4OS [M+H]+: 511.0893, Found: 511.0890.

2-(((4-Chloro-5-(3,4-difluorophenyl)-1H-pyrazol-3-yl)methyl)thio)-6-(cyclohexylmethyl)-5-ethyl-pyrimidin-4(3H)-one (I-33)

White solid; Yield 69%; m.p. 228–229 °C; 1H n class="Chemical">NMR (300 MHz, DMSO-d6, ppm) δ 0.92–0.97 (t, 3H, J = 7.2 Hz, CH3), 1.02–1.04 (m, 2H, cyclohexyl-H), 1.12–1.16 (m, 3H, cyclohexyl-H), 1.52–1.55 (m, 5H, cyclohexyl-H), 1.72 (m, 1H, cyclohexyl-H), 2.33–2.35 (m, 2H, CH2-cyclohexyl), 2.33–2.35 (m, 2H, CH2), 4.42 (s, 2H, CH2-S), 7.51–7.57 (m, 1H, Ph-H), 7.64–7.73 (m, 1H, Ph-H), 7.73–7.80 (m, 1H, Ph-H), 13.00 (br, 2H, 2NH); 13C NMR (75 MHz, DMSO-d6, ppm) δ 13.12, 17.98, 23.26, 25.70 (2), 25.83 (2), 32.59, 36.52, 40.75, 105.29 (2), 115.24 (d, 2JCF = 18.8 Hz), 117.92 (d, 2JCF = 17.3 Hz), 121.10, 123.29 (2C), 147.84, 149.22 (dd, 1JCF = 245.8 Hz, 2JCF = 12.8 Hz), 149.38 (dd, 1JCF = 243.0 Hz, 2JCF = 12.8 Hz), 155.79, 161.07, 162.95. HR-MS: Calcd. for C23H25ClF2N4OS [M+H]+: 479.1484, Found: 479.1482.

2-(((4-Chloro-5-(3-chloro-4-methoxyphenyl)-1H-pyrazol-3-yl)methyl)thio)-6-(cyclohexylmethyl)-5-ethyl-pyrimidin-4(3H)-one (I-34)

White solid; Yield: 53%; m.p. 303–305 °C; 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.88 (m, 2H, cyclohexyl-H), 0.93–0.98 (t, 3H, J = 6.9 Hz, CH3), 1.04–1.07 (m, 3H, cyclohexyl-H), 1.54–1.57 (m, 5H, cyclohexyl-H), 1.74 (m, 1H, cyclohexyl-H), 2.34–2.36 (m, 2H, CH2-cyclohexyl), 2.34–2.36 (m, 2H, CH2), 3.89 (s, 3H, OCH3), 4.42 (s, 2H, CH2-S), 7.24–7.27 (s, 1H, Ph-H), 7.72–7.81 (m, 2H, Ph-H), 12.52–13.21 (br, 2H, 2NH); 13C NMR (100 MHz, DMSO-d6, ppm) δ 13.18, 17.99, 23.71, 25.72 (2), 25.86 (2), 32.61, 36.52, 40.24, 56.15, 104.76, 112.91, 121.29, 123.5, 126.41, 127.59, 127.81, 154.49, 141.36, 142.55, 155.96, 160.95, 162.96; HR-MS: Calcd. for C24H28Cl2N4O2S [M+H]+: 507.1388, Found: 507.1384.

6-(Cyclohexylmethyl)-5-methyl-2-(((5-phenyl-1H-pyrazol-3-yl)methyl)thio)pyrimidin-4(3H)-one (I-35)

White solid; Yield: 85%; m.p. 204–206 °C; 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.94–1.00 (m, 2H, cyclohexyl-H), 1.08–1.11 (m, 3H, cyclohexyl-H), 1.58–1.73 (m, 6H, cyclohexyl-H), 1.87 (s, 3H, CH3), 2.39–2.41 (d, 2H, J = 6.7 Hz, CH2-cyclohexyl), 4.38 (s, 2H, CH2-S), 6.59 (s, 1H, pyrazole-H), 7.28–7.30 (m, 1H, Ph-H), 7.36–7.41 (t, 2H, J = 7.2 Hz, Ph-H), 7.70–7.72 (d, 2H, Ph-H), 12.84 (br, 2H, 2NH); 13C NMR (100 MHz, DMSO-d6, ppm) δ 10.47, 25.67 (2), 25.87 (3), 32.61, 36.68, 41.49, 101.61, 115.07, 124.96 (2), 127.66 (2), 128.68, 131.03 (2), 156.20, 161.68,163.36; HR-MS: Calcd. for C22H26N4OS [M+H]+: 395.1906, Found: 395.1903.

6-(Cyclohexylmethyl)-2-(((5-(4-methoxyphenyl)-1H-pyrazol-3-yl)methyl)thio)-5-methyl-pyrimidin-4(3H)-one (I-36)

White solid; Yield: 62%; m.p. 233–235 °C; 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.95–0.99 (m, 2H, cyclohexyl-H), 1.11–1.13 (m, 3H, cyclohexyl-H), 1.59–1.63 (m, 6H, cyclohexyl-H), 1.88 (s, 3H, CH3), 2.39–2.42 (m, 2H, CH2-cyclohexyl), 3.77 (s, 3H, OCH3), 4.36 (s, 2H, CH2-S), 6.49 (s, 1H, pyrazole-H), 6.95–6.98 (m, 2H, Ph-H), 7.62–7.65 (m, 2H, Ph-H), 12.70 (br, 2H, 2NH); 13C NMR (100 MHz, DMSO-d6, ppm) δ 10.46, 25.68 (2), 25.89 (3), 32.61, 36.66, 41.46, 55.10, 100.90, 114.14 (2), 115.08, 123.60, 126.33 (2), 145.50, 156.23, 158.92, 161.54, 163.36; HR-MS: Calcd. for C23H28N4O2S [M+H]+: 425.2011, Found: 425.2009.

6-(Cyclohexylmethyl)-2-(((5-(4-fluorophenyl)-1H-pyrazol-3-yl)methyl)thio)-5-methyl-pyrimidin-4(3H)-one (I-37)

White solid; Yield: 87%; m.p. 186–188 °C; 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.93–0.97 (m, 2H, cyclohexyl-H), 1.07–1.10 (m, 3H, cyclohexyl-H), 1.57–1.60 (m, 6H, cyclohexyl-H), 1.88 (s, 3H, CH3), 2.38–2.41 (d, 2H, J = 6.9 Hz, CH2-cyclohexyl), 4.38 (s, 2H, CH2-S), 6.58 (s, 1H, pyrazole-H), 7.20–7.26 (m, 2H, J = 8.8 Hz, Ph-H), 7.73–7.77 (m, 2H, Ph-H), 12.77 (br, 1H, NH); 13C NMR (100 MHz, DMSO-d6, ppm) δ 10.97, 25.95, 26.18 (2), 26.37, 33.09 (2), 37.17, 41.98, 102.13, 116.08 (2C, d, 2JCF = 21.4 Hz), 125.44, 127.47 (2C, d, 3JCF = 8.1 Hz), 128.19, 129.21, 139.44, 149.38, 162.15 (d, 1JCF = 243.0 Hz), 162.17, 163.84. HR-MS: Calcd. for C22H25FN4OS [M+H]+: 413.1811, Found: 413.1807.

6-(Cyclohexylmethyl)-2-(((5-phenyl-1H-pyrazol-3-yl)methyl)thio)pyrimidin-4(3H)-one (I-38)

White solid; Yield: 83%; m.p. 193–195 °C; 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.89–0.97 (m, 2H, cyclohexyl-H), 1.09–1.17 (m, 3H, cyclohexyl-H), 1.58–1.62 (m, 6H, cyclohexyl-H), 2.32–2.34 (d, 2H, J = 6.93 Hz, CH2-cyclohexyl), 4.40 (s, 2H, CH2-S), 5.96 (s, 1H, pyrimidone-H), 6.61 (s, 1H, pyrazole-H), 7.27–7.32 (t, 1H, J = 7.3 Hz, Ph-H), 7.37–7.42 (t, 2H, J = 7.5 Hz, Ph-H), 7.70–7.72 (d, 2H, Ph-H), 12.80 (br, 2H, 2NH); 13C NMR (100 MHz, DMSO-d6, ppm) δ 25.24 (2), 25.87 (3C), 32.42, 36.15, 44.36, 101.74, 107.34, 124.94 (2), 127.73 (3), 128.73, 161.53, 163.53, 166.80; HR-MS: Calcd. for C21H24N4OS [M+H]+: 381.1749, Found: 381.1747.

6-(Cyclohexylmethyl)-2-(((5-(4-methoxyphenyl)-1H-pyrazol-3-yl)methyl)thio)pyrimidin-4(3H)-one (I-39)

White solid; Yield: 47%; m.p. 184–186 °C; 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.90–0.97 (m, 2H, cyclohexyl-H), 1.10–1.17 (m, 3H, cyclohexyl), 1.59–1.72 (m, 6H, cyclohexyl), 2.32–2.34 (d, 2H, J = 6.84 Hz, CH2-cyclohexyl), 3.77 (s, 3H, OCH3), 4.38 (s, 2H, CH2-S), 5.96 (s, 1H, pyrimidone-H), 6.50 (s, 1H, pyrazole-H), 6.95–6.98 (d, 2H, J = 8.7 Hz, Ph-H), 7.62–7.65 (m, 2H, Ph-H), 12.72 (br, 2H, NH); 13C NMR (100 MHz, DMSO-d6, ppm) δ 25.55 (2), 25.87 (3), 32.43 (2), 36.13, 44.38, 55.08, 100.97, 107.36, 114.13 (2), 123.45, 126.32 (2), 145.47, 158.90 (2), 163.62, 166.72; HR-MS: Calcd. for C22H26N4O2S [M+H]+: 411.1855, Found: 411.1851.

6-(Cyclohexylmethyl)-2-(((5-(4-fluorophenyl)-1H-pyrazol-3-yl)methyl)thio)pyrimidin-4(3H)-one (I-40)

White solid; Yield: 89%; m.p. 203–205 °C. 1H n class="Chemical">NMR (400 MHz, DMSO-d6, ppm) δ 0.88–0.92 (m, 2H, cyclohexyl-H), 1.08–1.11 (m, 3H, cyclohexyl-H), 1.57–1.61 (m, 6H, cyclohexyl-H), 2.32–2.34 (d, 2H, J = 6.8 Hz, CH2-cyclohexyl), 4.40 (s, 2H, CH2-S), 5.96 (s, 1H, pyrimidone-H), 6.59 (s, 1H, pyrazole-H), 7.20–7.26 (m, 2H, Ph-H), 7.73–7.78 (m, 2H, Ph-H), 12.79 (br, 1H, NH); 13C NMR (100 MHz, DMSO-d6, ppm) δ 26.03 (2), 26.37, 32.92 (2), 36.65, 43.10, 44.86, 102.22, 105.54, 115.94 (d, 2JCF = 21.0 Hz), 116.10 (d, 2JCF = 21.5 Hz), 125.46, 127.49 (d, 3JCF = 8.1 Hz), 127.62 (d, 3JCF = 8.0 Hz), 129.68, 139.46, 149.38, 160.94, 162.28 (d, 1JCF = 242.8 Hz), 163.37; HR-MS: Calcd. for C21H23FN4OS [M+H]+: 399.1655, Found: 399.1652.

Biology assay

Cells and viruses

C8166 and TZM-bl cells were kindly provided by the AIDS Reagent Project, the UK Medical Research Council (MRC). PBMCs were isolated by Ficoll-Hypaque method from whole blood collected from healthy n class="Species">donor. The C8166 cells and PBMCs were maintained at 37 °C in 5% CO2 in RPMI-1640 medium supplemented with 10% heat-inactivating FBS (Gibco, Waltham, MA, USA). The TZM-bl cell was cultured in DMEM medium. In addition, wild-type strains HIV-1IIIB and HIV-1Ba-L, and HIV-1 resistant strains HIV-1A17, HIV-174V and HIV-1RF/V82F/184V were obtained from the NIH AIDS Research and Reference Reagent Program (Bethesda, Maryland, ME, USA). Clinically isolated HIV strains, including HIV-1TC-1 and HIV-1WAN were isolated from AIDS patients without HAART in Yunnan, China.

Anti-HIV-1 activity assay

The antiviral assay based on the viral cytopathic effect (CPE) was performed on all compounds followed by cytotoxic detection by using n class="Chemical">MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide, Thiazolyl Blue Tetrazolium Bromide) colorimetric reduction as previous reported. Briefly, HIV-1IIIB, C8166 cells and compounds were co-cultured for 3 days. The antiviral effect and cytotoxicity of each compound was analyzed by the inhibition of syncytia formation and the MTT assay. The EC50, CC50 and SI were provided. The activity against different HIV-1 strains of the compounds with high SI was assessed by different detection system. The anti-HIV-1Ba-L activity was measured by a luciferase gene expression assay in TZM-bl cells as follows: cells (2 × 104 per well) and viruses were incubated in 96-well plates with a multiplicity of infection (MOI) of 0.1 in the presence or absence of serial dilutions of the test compound. After 3 days post-treatment, the luciferase activity was measured quantitatively by relative fluorescence units, using a Promega's luciferase activity assay system. The inhibitory activities against resistant and clinical strains were determined as previously described. C8166 cells were infected with HIV-1A17, HIV-174V or HIV-1RF/V82F/184V, and PHA-stimulated PBMCs were incubated with HIV-1WAN or HIV-1TC-1 in RPMI-1640 at different serial dilutions of the compounds with a MOI of 0.1. Each assay included NVP as a positive control.

In-vitro HIV-1 RT inhibitory assay

Reverse transcriptase assay was performed using the Reverse Transcriptase Assay Kit (Roche, Maical">nnheim, Germany) accorn class="Gene">ding to the manufacturer's instructions. Briefly, the compounds I-11 and I-12 were dissolved in DMSO and stored at −4 °C. The compounds were then incubated with DIG-labeled reaction mixture at 37 °C for 2 h, then anti-DIG-POD solution was added, followed by substrate ABTS. Foscarnet was used as a positive control. The absorbency at 405 nm was read on Bio-Tek ELx 800 ELISA reader (Winooski, VT, USA),.

Pharmacokinetics assays

The mice used iical">n this study were housed aical">nd haical">ndled strictly iical">n accordance with the guideliical">nes set by the Associatioical">n for the Assessment aical">nd Accreditatioical">n of Laboratory Animal Care (Fre-derick, MD, USA). All animal experiments were coical">nducted iical">n accordance with the protocol approved by the Institutioical">nal Animal Care aical">nd Use Committee (IACUC) of Shaical">nghai Chempartical">ner Co., Ltd. Sample collectioical">n: n class="Chemical">six male Sprague–Dawley rats (200–230 g, 6–8 weeks) were divided into two groups (n = 3/group). Animals in group 1 received I-11 i.v. at 1 mg/kg while animals in group 2 received I-11 orally at 5 mg/kg (dissolved in 5% DMSO+5% Solutol HS 15 + 90% saline, strength: 0.5 mg/mL). Post-dosing serial blood samples (150 μL) were collected through tail vein into polypropylene tubes containing EDTA-K2 solutions as an anti-coagulant at 0.083, 0.25, 0.5, 1, 3, 6, 24 h (for i.v. arm) and 0.25, 0.5, 1, 3, 6, 24 h (for p.o. arm). Plasma was harvested by centrifuging the blood at 2000×g for 5 min and stored at approximately −70 °C until analysis.

LC–MS/MS method: LC–MS/MS system comprised an ACQUITY Ultra-performance liquid chromatography system (Waters, MA, USA) aical">nd a QTRAP-5500 mass spectrometer (AB Sciex Instruments) with aical">n En class="Chemical">SI ion source (Redwood city, CA, USA). The data acquisition and control system were created by using Analyst 1.5.1 software from AB SCIEX (Ontario, Canada). Chromatographic separation on an ACQUITY UPLC BEH C18 (2.1 mm×50 mm, 1.7 μm), mobile phase A (100% water containing 0.025% formic acid and 1 mmol/L ammonium acetate), mobile phase B (100% methanol containing 0.025% formic acid and 1 mmol/L ammonium acetate). The column was eluted at a flow rate of 0.6 mL/min in a gradient program consisting of 10% phase B (0–0.2 min), from 10% to 95% B (0.2–1.2 min), 95% B (1.20–1.60 min), from 95% to 10% B (1.60–1.61 min), 10% B (1.61–2 min). For I-11, the retention time for the analyte and IS (glipizide) were 1.46 min and 1.23 min respectively, injection volume is 0.5 μL. The precursor product ion pair was m/z 425.30 → 173.10 for I-11, m/z 446.20 → 321.10 for Diclofenac.

Standard curve preparation: Stock solution of I-11 were prepared at 1 mg/mL in DMSO. The stock solutioical">n was diluted with n class="Chemical">MeOH:H2O (7:3, v/v) to preparing serial working solution (20, 60, 200, 600, 2000, 6000, 20,000, 56,000, 60,000 ng/mL), 3 μL working solution was spiked into 57 μL male Sprague–Dawley rat plasma to obtain calibration standard curve (1, 2, 10, 30, 100, 300, 1000, 2700 and 3000 ng/mL).

Sample preparation: aliquot of 30 μL sample was added with 200 μL IS (glipizide, 20 ng/mL iical">n n class="Gene">ACN). The mixture was vortexed for 10 min at 750 rpm (Microporous Quick Shaker QB-9002, Changzhou, China) and centrifuged at 6000 rpm (Thermo Scientific Changzhou Multifuge X3R Centrifuge, Waltham, MA, USA) for 10 min. Aliquot of 0.5 μL supernatant was injected for LC–MS/MS analysis. For 10-fold diluted plasma samples, an aliquot of 3 μL plasma sample was diluted with 27 μL blank plasma, mixed well to achieve a dilution factor of 10. The subsequent processing procedure was the same as the un-diluted plasma samples.

Molecular docking

Molecular simulations were performed un class="Chemical">sing AutoDock4.2 program. All the molecules for docking were optimized for 5000-generation till the maximum derivative of energy became 0.005 kcal/mol·Å using the Tripos force field. Charges were computed and added according to Gasteiger-Huckel parameters. The published 3D crystal structure of the HIV-1 RT with TNK-651 complex was retrieved from the Protein Data Bank (PDB code: 1RT2). The molecules and proteins were prepared by using the AutoDockTools 1.5.4 (ADT). The bound ligand was extracted from the complexes, water molecules were removed, hydrogen atoms were automatically added, side chain amides and side chains bumps were fixed, and charges and atom types were assigned according to AMBER force field. Then, 100 separate docking calculations were performed using the reported modeling protocol.