Joint response of chemistry and functional microbial community to oxygenation of the reductive confined aquifer.

Oxygen can enter into reductive aquifer through natural and artificial processes. However, the joint response of groundwater chemistry and functional microbial communities to oxygenation is not well understood due to the gap between taxonomic and functional microbial composition. Here, two wells named CZK15 and CZK22 at the second confined aquifer in Central China were in situ aerated, and the chemical parameters of groundwater and microbial communities in bio-trapping sand sediment were analysed during aeration. The microbial metabolic functions related to C, N, S, Fe transformation were predicted by Functional Annotation of Prokaryotic Taxa (FAPROTAX) approach and some key functional genes, such as phe, nah, narG, and soxB were verified by the real-time quantitative Polymerase Chain Reaction (qPCR) method. The biomass was promoted, microbial diversity fluctuated, and microbial composition changed remarkably with aeration mainly constrained by reduction-oxidation (redox) variation and SO42- concentration. Among functional microbes, aerobic chemoheterotrophs including aromatic compound degraders (also especially for relative abundance of phe and some nah gene) and methylotrophs are dramatically enriched interpreting dissolved oxygen (DO) consumption and total organic carbon (TOC) decomposing in sediment. Whilst fermenters and methanogen expectedly decreased during aeration. Denitrifying microbes and narG gene relative abundance increased corresponding to the NO3- increase after aeration, while microbes for N2 fixation, ammonification, and nitrification decreased relating to the source of NH4+. The sulfide oxidation causing increased SO42- was reflected by the blooming of sulfur-oxidizing microbes and soxB gene. Some sulfate reducers persisted in sediment after aeration due to sufficient SO42- as substrate. Fe(II) was mainly chemically oxidized as iron-oxidizing microbes were of low abundance and tended to decrease with aeration. The iron-reducing bacteria Geobacteraceae increased with aeration corresponding to the increased Fe(III) oxides formation. The findings of this study could have important implications in understanding the biogeochemical behaviours with cyclic redox conditions.