Jun Yang1, Tao Qin2, Shanshan Liu2, Hui Tang2, Mengqing Liu3, Qian Wang1. 1. School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong 510515, China. 2. Department of Laboratory Medicine, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, China. 3. The Second Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, China.
Abstract
Aim: The aim of this study was to investigate the association of miR-1275 rs16759, IGF2BP1 rs11079850 and IGF2BP3 rs34414305 with hepatocellular carcinoma (HCC) risk. Materials & methods: Genotyping of the rs16759 and rs11079850 was performed using a Taqman assay and genotyping of the rs34414305 was performed using PCR. Relative expression of miR-1275, IGF2BP1 and IGF2BP3 was examined using quantitative PCR. Results: Comparison of the rs16759GG, CG/GG and CC genotype showed an increased risk of HCC. When comparing G with C allele, a significantly increased risk of HCC was also found. The rs16759, rs11079850 and rs34414305 had combined the interactive effects on the carcinogenesis of HCC. Moreover, the rs34414305 Del/ATT-Del/Del carriers displayed lower levels of IGF2BP3. Conclusion: The rs16759, rs11079850 and rs34414305 may singly and interactively contribute to carcinogenesis of HCC.
Aim: The aim of this study was to investigate the association of miR-1275rs16759, IGF2BP1rs11079850 and IGF2BP3rs34414305 with hepatocellular carcinoma (HCC) risk. Materials & methods: Genotyping of the rs16759 and rs11079850 was performed using a Taqman assay and genotyping of the rs34414305 was performed using PCR. Relative expression of miR-1275, IGF2BP1 and IGF2BP3 was examined using quantitative PCR. Results: Comparison of the rs16759GG, CG/GG and CC genotype showed an increased risk of HCC. When comparing G with C allele, a significantly increased risk of HCC was also found. The rs16759, rs11079850 and rs34414305 had combined the interactive effects on the carcinogenesis of HCC. Moreover, the rs34414305 Del/ATT-Del/Del carriers displayed lower levels of IGF2BP3. Conclusion: The rs16759, rs11079850 and rs34414305 may singly and interactively contribute to carcinogenesis of HCC.