Boldbaatar Gantuya1,2, Hashem B El Serag3, Takashi Matsumoto4, Nadim J Ajami5, Tomohisa Uchida6, Khasag Oyuntsetseg1,2, Dashdorj Bolor7, Yoshio Yamaoka3,4. 1. Department of Gastroenterology, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia. 2. Endoscopy Unit, Mongolia-Japan Teaching Hospital, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia. 3. Department of Medicine, Gastroenterology and Hepatology Section, Baylor College of Medicine, Houston, TX, USA. 4. Department of Environmental and Preventive Medicine, Oita University of Medicine, Yufu, Japan. 5. Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, USA. 6. Department of Molecular Pathology, Oita University of Medicine, Yufu, Japan. 7. Department of Endoscopy, Mongolian National Cancer Center Hospital, Ulaanbaatar, Mongolia.
Abstract
BACKGROUND: Incidence and mortality of gastric cancer (GC) are high in Mongolia despite Helicobacter pylori in the Mongolian population being less virulent. AIM: To evaluate gastric bacterial microbiota profiles in patients with GC and its precursor histological conditions. METHODS: We conducted a case-control study among 48 GC and 120 noncancer patients (20 normal gastric mucosa [control], 20 gastritis, 40 with atrophy and 40 intestinal metaplasia [IM]). We performed 16S rRNA gene amplicon sequencing and compared taxonomic and functional prediction profiles based on the diagnosis group and H pylori infection status. RESULTS: The highest overall bacterial alpha diversity metrics were observed in the control group, followed by the IM and cancer groups. The gastritis and atrophy groups had the least diversity. Lactobacilli and Enterococci were the dominant genus in several cancer patients especially in the absence of H pylori. In addition, Carnobacterium, Glutamicibacter, Paeniglutamicibacter, Fusobacterium and Parvimonas were associated with GC regardless of H pylori infection. Firmicutes were decreased in the gastritis and atrophy groups and increased in the IM and cancer groups. The functional metabolic activity of the Embden-Meyerhof-Parnas pathway and the utilization of sugar, were significantly increased in cancer group compared with the noncancer group. CONCLUSION: Microbial factors other than H pylori may play a role in Mongolian GC. We identified novel associations between GC and the genera Enterococcus, Lactobacillus, Carnobacterium, Glutamicibacter, Paeniglutamicibacter, Fusobacterium, and Parvimonas.
BACKGROUND: Incidence and mortality of gastric cancer (GC) are high in Mongolia despite Helicobacter pylori in the Mongolian population being less virulent. AIM: To evaluate gastric bacterial microbiota profiles in patients with GC and its precursor histological conditions. METHODS: We conducted a case-control study among 48 GC and 120 noncancer patients (20 normal gastric mucosa [control], 20 gastritis, 40 with atrophy and 40 intestinal metaplasia [IM]). We performed 16S rRNA gene amplicon sequencing and compared taxonomic and functional prediction profiles based on the diagnosis group and H pylori infection status. RESULTS: The highest overall bacterial alpha diversity metrics were observed in the control group, followed by the IM and cancer groups. The gastritis and atrophy groups had the least diversity. Lactobacilli and Enterococci were the dominant genus in several cancer patients especially in the absence of H pylori. In addition, Carnobacterium, Glutamicibacter, Paeniglutamicibacter, Fusobacterium and Parvimonas were associated with GC regardless of H pylori infection. Firmicutes were decreased in the gastritis and atrophy groups and increased in the IM and cancer groups. The functional metabolic activity of the Embden-Meyerhof-Parnas pathway and the utilization of sugar, were significantly increased in cancer group compared with the noncancer group. CONCLUSION: Microbial factors other than H pylori may play a role in Mongolian GC. We identified novel associations between GC and the genera Enterococcus, Lactobacillus, Carnobacterium, Glutamicibacter, Paeniglutamicibacter, Fusobacterium, and Parvimonas.
Authors: Pierre Sonveaux; Tamara Copetti; Christophe J De Saedeleer; Frédérique Végran; Julien Verrax; Kelly M Kennedy; Eui Jung Moon; Suveera Dhup; Pierre Danhier; Françoise Frérart; Bernard Gallez; Anthony Ribeiro; Carine Michiels; Mark W Dewhirst; Olivier Feron Journal: PLoS One Date: 2012-03-13 Impact factor: 3.240