Virinder Sarhadi1, Leo Lahti2, Farideh Saberi3, Omar Youssef1, Arto Kokkola4, Tiina Karla5, Milja Tikkanen5, Hilpi Rautelin6, Pauli Puolakkainen5, Rasoul Salehi3,7, Sakari Knuutila8. 1. Department of Pathology, Faculty of Medicine, University of Helsinki, Helsinki, Finland. 2. Department of Future Technologies, University of Turku, Turku, Finland. 3. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. 4. The HUCH Gastrointestinal Clinic, University Central Hospital of Helsinki, Helsinki, Finland. 5. Thermo Fisher Scientific, Vantaa, Finland. 6. Department of Medical Sciences, Clinical Microbiology, Uppsala University, Uppsala, Sweden. 7. Pediatric Inherited Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, Iran. 8. Department of Pathology, Faculty of Medicine, University of Helsinki, Helsinki, Finland sakari.knuutila@helsinki.fi.
Abstract
BACKGROUND/AIM: Gut microbiota plays an important role in colorectal cancer (CRC) and its composition in CRC patients can be influenced by ethnicity and tumour genomics. Herein, the aim was to study the possible associations of ethnicity and gene mutations with the gut microbiota in CRC patients. MATERIALS AND METHODS: Bacterial composition in stool samples of 83 CRC patients and 60 controls from Iran and Finland was studied by 16S rRNA gene sequencing. The association of gut microbiota composition with CRC, host mutations in KRAS, NRAS and TP53, and ethnicity analysed. RESULTS: Beta diversity analysis indicated significant differences between the Iranian and Finnish gut microbiota composition, in both controls and patients' groups. The Iranian controls had higher abundance of Prevotella and lower abundance of Bacteroides compared to the Finnish controls, while the Finnish patients had higher abundance of Clostridium compared to Iranian patients. Abundance of Ruminococcus was higher in patients compared to the controls. Higher abundances of Herbaspirillum, Catenibacterium and lower abundances of Barnesiella were associated with mutations in NRAS, TP53, and RAS respectively. CONCLUSION: A possible link of host gene mutations with gut bacterial composition is suggested. Copyright
BACKGROUND/AIM: Gut microbiota plays an important role in colorectal cancer (CRC) and its composition in CRCpatients can be influenced by ethnicity and tumour genomics. Herein, the aim was to study the possible associations of ethnicity and gene mutations with the gut microbiota in CRCpatients. MATERIALS AND METHODS: Bacterial composition in stool samples of 83 CRCpatients and 60 controls from Iran and Finland was studied by 16S rRNA gene sequencing. The association of gut microbiota composition with CRC, host mutations in KRAS, NRAS and TP53, and ethnicity analysed. RESULTS: Beta diversity analysis indicated significant differences between the Iranian and Finnish gut microbiota composition, in both controls and patients' groups. The Iranian controls had higher abundance of Prevotella and lower abundance of Bacteroides compared to the Finnish controls, while the Finnish patients had higher abundance of Clostridium compared to Iranian patients. Abundance of Ruminococcus was higher in patients compared to the controls. Higher abundances of Herbaspirillum, Catenibacterium and lower abundances of Barnesiella were associated with mutations in NRAS, TP53, and RAS respectively. CONCLUSION: A possible link of host gene mutations with gut bacterial composition is suggested. Copyright
Authors: Sofie Bosch; Animesh Acharjee; Mohammed N Quraishi; Patricia Rojas; Abdellatif Bakkali; Erwin Ew Jansen; Marina Brizzio Brentar; Johan Kuijvenhoven; Pieter Stokkers; Eduard Struys; Andrew D Beggs; Georgios V Gkoutos; Tim Gj de Meij; Nanne Kh de Boer Journal: Gut Microbes Date: 2022 Jan-Dec