Rongzhi Zhang1,2,3, Siyuan Huang4, Shiming Li4, Guoqi Song5,6,7, Yulian Li5,6,7, Wei Li5,6,7, Jihu Li5,6,7, Jie Gao5,6,7, Tiantian Gu5,6,7, Dandan Li5,6,7, Shujuan Zhang8,9,10, Genying Li11,12,13. 1. Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, 250100, Shandong, China. zhangrongzhi1981@126.com. 2. Key Laboratory of Wheat Biology and Genetic Improvement on North Yellow and Huai River Valley, Ministry of Agriculture, Jinan, 250100, Shandong, China. zhangrongzhi1981@126.com. 3. National Engineering Laboratory for Wheat and Maize, Jinan, 250100, Shandong, China. zhangrongzhi1981@126.com. 4. BGI Institute of Applied Agriculture, BGI-Shenzhen, Shenzhen, 518120, China. 5. Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, 250100, Shandong, China. 6. Key Laboratory of Wheat Biology and Genetic Improvement on North Yellow and Huai River Valley, Ministry of Agriculture, Jinan, 250100, Shandong, China. 7. National Engineering Laboratory for Wheat and Maize, Jinan, 250100, Shandong, China. 8. Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, 250100, Shandong, China. zsjhappy@163.com. 9. Key Laboratory of Wheat Biology and Genetic Improvement on North Yellow and Huai River Valley, Ministry of Agriculture, Jinan, 250100, Shandong, China. zsjhappy@163.com. 10. National Engineering Laboratory for Wheat and Maize, Jinan, 250100, Shandong, China. zsjhappy@163.com. 11. Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, 250100, Shandong, China. lgy111@126.com. 12. Key Laboratory of Wheat Biology and Genetic Improvement on North Yellow and Huai River Valley, Ministry of Agriculture, Jinan, 250100, Shandong, China. lgy111@126.com. 13. National Engineering Laboratory for Wheat and Maize, Jinan, 250100, Shandong, China. lgy111@126.com.
Abstract
BACKGROUND: PhasiRNAs (phased secondary siRNAs) play important regulatory roles in the development processes and biotic or abiotic stresses in plants. Some of phasiRNAs involve in the reproductive development in grasses, which include two categories, 21-nt (nucleotide) and 24-nt phasiRNAs. They are triggered by miR2118 and miR2275 respectively, in premeiotic and meiotic anthers of rice, maize and other grass species. Wheat (Triticum aestivum) with three closely related subgenomes (subA, subB and subD), is a model of allopolyploid in plants. Knowledge about the role of phasiRNAs in the inflorescence development of wheat is absent until now, and the evolution of PHAS loci in polyploid plants is also unavailable. RESULTS: Using 261 small RNA expression datasets from various tissues, a batch of PHAS (phasiRNA precursors) loci were identified in the young spike of wheat, most of which were regulated by miR2118 and miR2275 in their target site regions. Dissection of PHAS and their trigger miRNAs among the diploid (AA and DD), tetraploid (AABB) and hexaploid (AABBDD) genomes of Triticum indicated that distribution of PHAS loci were dominant randomly in local chromosomes, while miR2118 was dominant only in the subB genome. The diversity of PHAS loci in the three subgenomes of wheat and their progenitor genomes (AA, DD and AABB) suggested that they originated or diverged at least before the occurrence of the tetraploid AABB genome. The positive correlation between the PHAS loci or the trigger miRNAs and the ploidy of genome indicated the expansion of genome was the major drive force for the increase of PHAS loci and their trigger miRNAs in Triticum. In addition, the expression profiles of the PHAS transcripts suggested they responded to abiotic stresses such as cold stress in wheat. CONCLUSIONS: Altogether, non-coding phasiRNAs are conserved transcriptional regulators that display quick plasticity in Triticum genome. They may be involved in reproductive development and abiotic stress in wheat. It could be referred to molecular research on male reproductive development in Triticum.
BACKGROUND: PhasiRNAs (phased secondary siRNAs) play important regulatory roles in the development processes and biotic or abiotic stresses in plants. Some of phasiRNAs involve in the reproductive development in grasses, which include two categories, 21-nt (nucleotide) and 24-nt phasiRNAs. They are triggered by miR2118 and miR2275 respectively, in premeiotic and meiotic anthers of rice, maize and other grass species. Wheat (Triticum aestivum) with three closely related subgenomes (subA, subB and subD), is a model of allopolyploid in plants. Knowledge about the role of phasiRNAs in the inflorescence development of wheat is absent until now, and the evolution of PHAS loci in polyploid plants is also unavailable. RESULTS: Using 261 small RNA expression datasets from various tissues, a batch of PHAS (phasiRNA precursors) loci were identified in the young spike of wheat, most of which were regulated by miR2118 and miR2275 in their target site regions. Dissection of PHAS and their trigger miRNAs among the diploid (AA and DD), tetraploid (AABB) and hexaploid (AABBDD) genomes of Triticum indicated that distribution of PHAS loci were dominant randomly in local chromosomes, while miR2118 was dominant only in the subB genome. The diversity of PHAS loci in the three subgenomes of wheat and their progenitor genomes (AA, DD and AABB) suggested that they originated or diverged at least before the occurrence of the tetraploid AABB genome. The positive correlation between the PHAS loci or the trigger miRNAs and the ploidy of genome indicated the expansion of genome was the major drive force for the increase of PHAS loci and their trigger miRNAs in Triticum. In addition, the expression profiles of the PHAS transcripts suggested they responded to abiotic stresses such as cold stress in wheat. CONCLUSIONS: Altogether, non-coding phasiRNAs are conserved transcriptional regulators that display quick plasticity in Triticum genome. They may be involved in reproductive development and abiotic stress in wheat. It could be referred to molecular research on male reproductive development in Triticum.
Entities:
Keywords:
Evolution; MicroRNAs; PHAS; PhasiRNAs; Wheat; Young spike
Authors: Jiali Yu; Dennis Bennett; Christopher Dardick; Tetyana Zhebentyayeva; Albert G Abbott; Zongrang Liu; Margaret E Staton Journal: Front Mol Biosci Date: 2021-04-09