Yuqing Sun1, Shaohui Zheng2,3, Long Liu1, Ying Kong1, Aiwei Zhang4, Kai Xu5,6, Cuiping Han7,8. 1. School of Medical Imaging, Xuzhou Medical University, Xuzhou, Jiangsu, 221004, People's Republic of China. 2. School of Medical Imaging, Xuzhou Medical University, Xuzhou, Jiangsu, 221004, People's Republic of China. shaohui19910@163.com. 3. Department of Radiology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People's Republic of China. shaohui19910@163.com. 4. Department of Radiology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People's Republic of China. 5. School of Medical Imaging, Xuzhou Medical University, Xuzhou, Jiangsu, 221004, People's Republic of China. xkpaper@163.com. 6. Department of Radiology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People's Republic of China. xkpaper@163.com. 7. School of Medical Imaging, Xuzhou Medical University, Xuzhou, Jiangsu, 221004, People's Republic of China. hancp@xzhmu.edu.cn. 8. Department of Radiology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People's Republic of China. hancp@xzhmu.edu.cn.
Abstract
Doxorubicin entrapped carbon dots (DOX-CDs) were prepared for bioimaging and enhanced intracellular drug delivery. The CDs were synthesized via the hydrothermal method using citrate and urea under 200 °C for 1 h. Then, DOX was successfully conjugated on the CDs via physicochemical interactions. The DOX-CDs exhibited good crystal structure, remarkable aqueous stability, excellent photoluminescence property, and a high quantum yield of 93%. The fluorescent images revealed that the DOX-CDs could be readily taken up by the cancer cells for cell labeling. Furthermore, endo-lysosomal pH-assisted DOX release behavior was observed from DOX-CDs, and the cytotoxicity of DOX-CDs was confirmed by the MTS assay against H0-8910 ovarian cancer cells. In addition, the CDs indicated bright fluorescent signal in the animal imaging test and demonstrated low toxicity after administration for 7 and 21 days. Therefore, the prepared CDs could be a promising imaging probe for biomedical imaging and intracellular drug delivery.
Doxorubicin entrapped n class="Chemical">carbon dots (DOX-CDs) were prepared for bioimaging and enhanced intracellular drug delivery. The CDs were synthesized via the hydrothermal method using citrate and urea under 200 °C for 1 h. Then, DOX was successfully conjugated on the CDs via physicochemical interactions. The DOX-CDs exhibited good crystal structure, remarkable aqueous stability, excellent photoluminescence property, and a high quantum yield of 93%. The fluorescent images revealed that the DOX-CDs could be readily taken up by the cancer cells for cell labeling. Furthermore, endo-lysosomal pH-assisted DOX release behavior was observed from DOX-CDs, and the cytotoxicity of DOX-CDs was confirmed by the MTS assay against H0-8910 ovarian cancer cells. In addition, the CDs indicated bright fluorescent signal in the animal imaging test and demonstrated low toxicity after administration for 7 and 21 days. Therefore, the prepared CDs could be a promising imaging probe for biomedical imaging and intracellular drug delivery.
Entities:
Keywords:
Bioimaging; DOX-CDs; Green fluorescent carbon dots; Targeted delivery
Authors: Asmita Dutta; Shimon T Y Trolles-Cavalcante; Annie Cleetus; Vered Marks; Alex Schechter; Richard D Webster; Arie Borenstein Journal: Nanoscale Adv Date: 2020-12-10