| Literature DB >> 32124327 |
Masaki Suimye Morioka1, Hideya Kawaji1,2,3, Hiromi Nishiyori-Sueki4, Mitsuyoshi Murata4, Miki Kojima-Ishiyama4, Piero Carninci4, Masayoshi Itoh5.
Abstract
Cap analysis of gene expression (CAGE) is an approach to identify and monitor the activity (transcription initiation frequency) of transcription start sites (TSSs) at single base-pair resolution across the genome. It has been effectively used to identify active promoter and enhancer regions in cancer cells, with potential utility to identify key factors to immunotherapy. Here, we overview a series of CAGE protocols and describe detailed experimental steps of the latest protocol based on the Illumina sequencing platform; both experimental steps (see Subheadings 3.1-3.11) and computational processing steps (see Subheadings 3.12-3.20) are described.Entities:
Keywords: CAGE; Enhancer; Promoter activity; Promoter-level expression analysis; TSS; Transcription initiation; Transcription start site; eRNA
Mesh：
Year: 2020 PMID： 32124327 DOI： 10.1007/978-1-0716-0327-7_20
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745