Literature DB >> 32119023

One-pot synthesis of heterodimeric agonists that activate the canonical Wnt signaling pathway.

Abhirup Mukherjee1, Mark E Stathos2, Chad Varner1, Ammar Arsiwala1, Steven Frey1, Yuge Hu1, David M Smalley3, David V Schaffer4, Ravi S Kane1.   

Abstract

Fragment antigen-binding domains (Fabs) from anti-Frizzled and anti-LRP6 monoclonal antibodies were conjugated using SpyTag-SpyCatcher chemistry via a one-pot reaction. The resulting synthetic heterodimeric agonist outperformed the natural ligand, Wnt-3a, in activating canonical Wnt signaling in mammalian cells. This approach should be broadly applicable to activate receptor-mediated cellular signaling.

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Year:  2020        PMID： 32119023      PMCID： PMC7179975          DOI： 10.1039/d0cc00920b

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  24 in total

1.  Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin.

Authors:  Bijan Zakeri; Jacob O Fierer; Emrah Celik; Emily C Chittock; Ulrich Schwarz-Linek; Vincent T Moy; Mark Howarth
Journal:  Proc Natl Acad Sci U S A       Date:  2012-02-24       Impact factor: 11.205

2.  Wnt signaling through inhibition of β-catenin degradation in an intact Axin1 complex.

Authors:  Vivian S W Li; Ser Sue Ng; Paul J Boersema; Teck Y Low; Wouter R Karthaus; Jan P Gerlach; Shabaz Mohammed; Albert J R Heck; Madelon M Maurice; Tokameh Mahmoudi; Hans Clevers
Journal:  Cell       Date:  2012-06-08       Impact factor: 41.582

3.  Post-translational palmitoylation and glycosylation of Wnt-5a are necessary for its signalling.

Authors:  Manabu Kurayoshi; Hideki Yamamoto; Shunsuke Izumi; Akira Kikuchi
Journal:  Biochem J       Date:  2007-03-15       Impact factor: 3.857

Review 4.  Glycogen synthase kinase-3 (GSK3): regulation, actions, and diseases.

Authors:  Eleonore Beurel; Steven F Grieco; Richard S Jope
Journal:  Pharmacol Ther       Date:  2014-11-27       Impact factor: 12.310

5.  Kinetic responses of β-catenin specify the sites of Wnt control.

Authors:  Ana R Hernández; Allon M Klein; Marc W Kirschner
Journal:  Science       Date:  2012-11-08       Impact factor: 47.728

6.  Monounsaturated fatty acid modification of Wnt protein: its role in Wnt secretion.

Authors:  Ritsuko Takada; Yoshinori Satomi; Tomoko Kurata; Naoto Ueno; Shigemi Norioka; Hisato Kondoh; Toshifumi Takao; Shinji Takada
Journal:  Dev Cell       Date:  2006-12       Impact factor: 12.270

7.  Phase II trial of the anti-CD19 bispecific T cell-engager blinatumomab shows hematologic and molecular remissions in patients with relapsed or refractory B-precursor acute lymphoblastic leukemia.

Authors:  Max S Topp; Nicola Gökbuget; Gerhard Zugmaier; Petra Klappers; Matthias Stelljes; Svenja Neumann; Andreas Viardot; Reinhard Marks; Helmut Diedrich; Christoph Faul; Albrecht Reichle; Heinz-August Horst; Monika Brüggemann; Dorothea Wessiepe; Chris Holland; Shilpa Alekar; Noemi Mergen; Hermann Einsele; Dieter Hoelzer; Ralf C Bargou
Journal:  J Clin Oncol       Date:  2014-11-10       Impact factor: 44.544

8.  Lentiviral vectors to probe and manipulate the Wnt signaling pathway.

Authors:  Christophe Fuerer; Roel Nusse
Journal:  PLoS One       Date:  2010-02-23       Impact factor: 3.240

9.  Glycosylation and palmitoylation of Wnt-3a are coupled to produce an active form of Wnt-3a.

Authors:  Hideyuki Komekado; Hideki Yamamoto; Tsutomu Chiba; Akira Kikuchi
Journal:  Genes Cells       Date:  2007-04       Impact factor: 1.891

10.  Glycogen synthase kinase 3 inhibitors induce the canonical WNT/β-catenin pathway to suppress growth and self-renewal in embryonal rhabdomyosarcoma.

Authors:  Eleanor Y Chen; Michael T DeRan; Myron S Ignatius; Kathryn Brooke Grandinetti; Ryan Clagg; Karin M McCarthy; Riadh M Lobbardi; Jillian Brockmann; Charles Keller; Xu Wu; David M Langenau
Journal:  Proc Natl Acad Sci U S A       Date:  2014-03-24       Impact factor: 11.205
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  5 in total

1.  Molecular architecture and modifications of full-length myocilin.

Authors:  Mackenzie D Martin; Dustin J E Huard; Ricardo C Guerrero-Ferreira; Ishani M Desai; Brett M Barlow; Raquel L Lieberman
Journal:  Exp Eye Res       Date:  2021-08-13       Impact factor: 3.770

Review 2.  Power to the protein: enhancing and combining activities using the Spy toolbox.

Authors:  Anthony H Keeble; Mark Howarth
Journal:  Chem Sci       Date:  2020-07-03       Impact factor: 9.825

Review 3.  Chemical and Enzymatic Methods for Post-Translational Protein-Protein Conjugation.

Authors:  Ross J Taylor; Michael B Geeson; Toby Journeaux; Gonçalo J L Bernardes
Journal:  J Am Chem Soc       Date:  2022-07-31       Impact factor: 16.383

4.  Gene Co-Expression Analysis of Human RNASEH2A Reveals Functional Networks Associated with DNA Replication, DNA Damage Response, and Cell Cycle Regulation.

Authors:  Stefania Marsili; Ailone Tichon; Deepali Kundnani; Francesca Storici
Journal:  Biology (Basel)       Date:  2021-03-13

Review 5.  Signalling dynamics in embryonic development.

Authors:  Katharina F Sonnen; Claudia Y Janda
Journal:  Biochem J       Date:  2021-12-10       Impact factor: 3.857
  5 in total

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