| Literature DB >> 32115466 |
Yuhui Men1, Xiaomin Zhou1, Zhijie Yan1, Linqiang Niu1, Yang Luo1, Jiamin Wang2, Jianhong Wang3.
Abstract
A near-infrared (NIR) and water-soluble probe was synthesized and studied for the detection of Cys/Hcy in aqueous solution, living cells and mice. The probe was composed of cyanine derivative as the NIR fluorescent reporting unit and pyrimidiny-thioether moiety as the Cys/Hcy responsive unit. Treatment with Cys/Hcy induced the formation of sulfur-substituted products, then intramolecular rearrangement reaction would occur to produce amino-substituted products and resulting in enhanced red fluorescence emissions. It could be applied to sense Cys/Hcy both in solution with the detection limit of 0.17 μM (or 0.32 μM) and in living cells. Cell imaging experiments proved that such a probe exhibited good cell penetration. In addition, the probe could detect Cys/Hcy in live mice with strong turn-on fluorescent response.Entities:
Keywords: Cys/Hcy; Near-infrared (NIR); cyanine; fluorescent probe; in vivo imaging
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Year: 2020 PMID： 32115466 DOI： 10.2116/analsci.20P016
Source DB: PubMed Journal: Anal Sci ISSN： 0910-6340 Impact factor: 1.967