Jin-Cherng Chen1,2, Pei-Shan Hsieh3, Shu-Min Chen3, Juen-Haur Hwang4,5,6. 1. Department of Neurosurgery, Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Chiayi, Taiwan, R.O.C. 2. School of Medicine, Tzu Chi University, Haulien, Taiwan, R.O.C. 3. Department of Medical Research, Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Chiayi, Taiwan, R.O.C. 4. School of Medicine, Tzu Chi University, Haulien, Taiwan, R.O.C. g120796@tzuchi.com.tw. 5. Department of Otolaryngology-Head and Neck Surgery, Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Chiayi, Taiwan, R.O.C. 6. Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan, R.O.C.
Abstract
BACKGROUND/AIM: The effects of cinnamaldehyde on glioma are still unclear. We aimed to investigate the effects of cinnamaldehyde on the viability and expression of chemokine receptors CXCR4 and CXCR7 in temozolomide (TMZ)-treated glioma cells. MATERIALS AND METHODS: Cell viability and CXCR4 and CXCR7 expression were measured by western blotting at 72 h after treatment with various concentrations of cinnamaldehyde and TMZ. RESULTS: Cell viability was significantly lower after treatment with 300 μM TMZ, 50 μM cinnamaldehyde, 75 μM cinnamaldehyde, or combined treatment with 300 μM TMZ plus 50 μM or 75 μM cinnamaldehyde than after no treatment (i.e., without TMZ or cinnamaldehyde); and significantly lower after combined treatment with 300 μM TMZ plus 75 μM cinnamaldehyde but not 50 μM cinnamaldehyde, than treatment with 300 μM TMZ alone. Western blotting showed that either single treatments or combined treatments had lower CXCR4 expression (compared to the no-treatment control). Compared to 300 μM TMZ alone, both combined treatment of 300 μM TMZ plus 50 μM cinnamaldehyde or 75 μM cinnamaldehyde had significantly lowered CXCR4 expression. However, CXCR7 expression was not significantly different in all groups. CONCLUSION: Cinnamaldehyde, acting with TMZ, reduces glioma cell viability possibly via decreasing CXCR4 expression. Copyright
BACKGROUND/AIM: The effects of cinnamaldehyde on glioma are still unclear. We aimed to investigate the effects of cinnamaldehyde on the viability and expression of chemokine receptors CXCR4 and CXCR7 in temozolomide (TMZ)-treated glioma cells. MATERIALS AND METHODS: Cell viability and CXCR4 and CXCR7 expression were measured by western blotting at 72 h after treatment with various concentrations of cinnamaldehyde and TMZ. RESULTS: Cell viability was significantly lower after treatment with 300 μM TMZ, 50 μM cinnamaldehyde, 75 μM cinnamaldehyde, or combined treatment with 300 μM TMZ plus 50 μM or 75 μM cinnamaldehyde than after no treatment (i.e., without TMZ or cinnamaldehyde); and significantly lower after combined treatment with 300 μM TMZ plus 75 μM cinnamaldehyde but not 50 μM cinnamaldehyde, than treatment with 300 μM TMZ alone. Western blotting showed that either single treatments or combined treatments had lower CXCR4 expression (compared to the no-treatment control). Compared to 300 μM TMZ alone, both combined treatment of 300 μM TMZ plus 50 μM cinnamaldehyde or 75 μM cinnamaldehyde had significantly lowered CXCR4 expression. However, CXCR7 expression was not significantly different in all groups. CONCLUSION:Cinnamaldehyde, acting with TMZ, reduces glioma cell viability possibly via decreasing CXCR4 expression. Copyright
Authors: Yi-fang Ping; Xiao-hong Yao; Jian-yong Jiang; Lin-tao Zhao; Shi-cang Yu; Tao Jiang; Marie C M Lin; Jian-hong Chen; Bin Wang; Rong Zhang; You-hong Cui; Cheng Qian; Ji ming Wang; Xiu-wu Bian Journal: J Pathol Date: 2011-05-27 Impact factor: 7.996
Authors: Céline Bruyère; Tatjana Mijatovic; Caroline Lonez; Sabine Spiegl-Kreinecker; Walter Berger; Richard E Kast; Jean-Marie Ruysschaert; Robert Kiss; Florence Lefranc Journal: Int J Oncol Date: 2011-03-08 Impact factor: 5.650